An irritable bowel syndrome subtype defined by species-specific alterations in faecal microbiota (original) (raw)
An irritable bowel syndrome subtype defined by species-specific alterations in faecal microbiota
- Ian B Jeffery1,
- Paul W O'Toole1,2,
- Lena Öhman3,
- Marcus J Claesson1,2,
- Jennifer Deane1,2,
- Eamonn M M Quigley2,
- Magnus Simrén3
- 1Department of Microbiology, University College Cork, Cork, Ireland
- 2Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
- 3Department of Internal Medicine, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Correspondence to Dr Paul W O'Toole, Department of Microbiology, University College Cork, 447 Food Science Building, Cork, Ireland; pwotoole{at}ucc.ie
Abstract
Background and aims Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder that may be triggered by enteric pathogens and has also been linked to alterations in the microbiota and the host immune response. The authors performed a detailed analysis of the faecal microbiota in IBS and control subjects and correlated the findings with key clinical and physiological parameters.
Design The authors used pyrosequencing to determine faecal microbiota composition in 37 IBS patients (mean age 37 years; 26 female subjects; 15 diarrhoea-predominant IBS, 10 constipation-predominant IBS and 12 alternating-type IBS) and 20 age- and gender-matched controls. Gastrointestinal and psychological symptom severity and quality of life were evaluated with validated questionnaires and colonic transit time and rectal sensitivity were measured.
Results Associations detected between microbiota composition and clinical or physiological phenotypes included microbial signatures associated with colonic transit and levels of clinically significant depression in the disease. Clustering by microbiota composition revealed subgroups of IBS patients, one of which (n=15) showed normal-like microbiota composition compared with healthy controls. The other IBS samples (n=22) were defined by large microbiota-wide changes characterised by an increase of _Firmicutes_-associated taxa and a depletion of _Bacteroidetes_-related taxa.
Conclusions Detailed microbiota analysis of a well-characterised cohort of IBS patients identified several clear associations with clinical data and a distinct subset of IBS patients with alterations in their microbiota that did not correspond to IBS subtypes, as defined by the Rome II criteria.
- Irritable bowel syndrome
- 16S ribosomal RNA
- sequence analysis
- human microbiome
- inflammatory bowel syndrome
- functional bowel disorder
- lamina proprial lymphocytes
- IBD
- inflammatory bowel disorders
- IBD models
- mucosa-associated lymphoid tissue
- mucosal immunology
- constipation
- gastroparesis
- intestinal transplantation
- gastric emptying
- quality of life
- motility disorders
- visceral sensitivity
- gastrointestinal motility
- functional dyspepsia
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Gut 2012; 61 i-ii Published Online First: 17 May 2012. doi: 10.1136/gutjnl-2012-302847