Clinical Implication of Serine Metabolism-Associated Enzymes in Colon Cancer (original) (raw)
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Research Articles| October 07 2015
aDepartments of Oncology/Hematology,
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aDepartments of Oncology/Hematology,
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cSurgery, Cancer Research Institute, and
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cSurgery, Cancer Research Institute, and
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cSurgery, Cancer Research Institute, and
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cSurgery, Cancer Research Institute, and
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eDepartment of Internal Medicine, Soonchunhyang University Gumi Hospital, Soonchunhyang University College of Medicine, Gumi, South Korea
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dInstitute of Life Science and Biotechnology, Kyungpook National University School of Medicine, Daegu, and
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aDepartments of Oncology/Hematology,
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Oncology (2015) 89 (6): 351–359.
Citation
Shinkyo Yoon, Jong Gwang Kim, An Na Seo, Soo Yeun Park, Hye Jin Kim, Jun Seok Park, Gyu Seog Choi, Ji Yun Jeong, Do Youn Jun, Ghil Suk Yoon, Byung Woog Kang; Clinical Implication of Serine Metabolism-Associated Enzymes in Colon Cancer. _Oncology 1 November 2015; 89 (6): 351–359. https://doi.org/10.1159/000439571
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Abstract
Purpose: Recently, enzymes of the serine synthetic pathway (SSP) have been suggested as key player in the metabolic adaptation of oncogenesis. We assessed the expression of enzymes of the SSP in colonic tumor tissue (TT) and paired normal tissue (pNT) and the prognostic implications. Methods: From 2006 to 2010, we included 486 patients with colon cancer who underwent curative surgery at Kyungpook National University Hospital. Phosphoglycerate dehydrogenase (PHGDH), pyruvate dehydrogenase kinase (PDK) 1, PDK2, pyruvate kinase M2 (PKM2), and phosphoserine aminotransferase (PSAT) expression were investigated by immunohistochemical staining (IHC) in TT and pNT. The IHC values were calculated by multiplying intensity by proportion. The final score was classified as follows: 0-2 as negative and 3-12 as positive. Results: During the median follow-up duration of 55.5 months (37.4-90.6), 78 patients experienced recurrence. The expression of PHGDH, PDK1, and PSAT was significantly higher in TT than pNT (p < 0.001 for each). The univariate analysis for relapse-free survival revealed that TT PDK2 positivity was the only positive prognostic factor (p = 0.023). However, the expression of TT PDK2 did not represent a prognostic value in multivariate analysis. Conclusions: In conclusion, PHGDH, PDK1, and PSAT were significantly increased in colonic TT compared with pNT. The prognostic implication of these enzymes needs to be further investigated.
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