Translocated EspF protein from enteropathogenic Escherichia coli disrupts host intestinal barrier function (original) (raw)
EspF is not required for attaching and effacing activity in an intestinal epithelial cell line. We reported previously that EspF is not required for EPEC to induce rearrangements of actin in infected human laryngeal carcinoma HEp-2 cells (9). To determine whether EspF is required for full attaching and effacing activity in differentiated intestinal epithelial cells, we infected polarized T84 intestinal epithelial cells with either wild-type EPEC strain E2348/69 or espF mutant strain UMD874 and determined the percentage of bacteria that were involved in attaching and effacing lesions by electron microscopy. Of a total of 192 wild-type bacteria observed in the samples, 95 (49%) were engaged in attaching and effacing activity. Similarly, of a total of 122 espF mutant bacteria observed, 65 (53%) were engaged in attaching and effacing activity (P = 0.59). Furthermore, we detected no qualitative difference in the nature of the attaching and effacing lesions induced by the wild-type and espF mutant strains (Figure 1).
Transmission electron micrographs of polarized T84 cells infected with (a) wild-type EPEC strain E2348/69 or (b) espF mutant strain UMD874. Typical attaching and effacing lesions are present in both micrographs (arrows). Bar, 1 μm.
EspF is translocated into epithelial cells. We reported previously that EspF is exported by EPEC via the type III secretion pathway (9). We used an adenylate cyclase reporter system (34) to determine whether EspF is targeted to the host cell cytoplasm. Since the B. pertussis adenylate cyclase requires calmodulin for activity, fusion proteins that contain the catalytic portion of this enzyme have activity only if they are directed to the cytoplasm of host cells where calmodulin is abundant. We constructed a plasmid that has a fusion between full-length espF and a portion of the cya gene encoding the catalytic domain of adenylate cyclase and introduced this plasmid into various EPEC strains. We also fused the first 73 codons of espF to cya, since in other systems the 5′ end of the gene is sufficient to allow effector molecules to be translocated via type III systems (35). We then infected HeLa cells with EPEC strains containing these constructs, prepared cell lysates, and assayed cAMP by ELISA. We found that cells infected with wild-type EPEC strain E2348/69 containing the full-length espF-cya fusion plasmid had levels of cAMP that were more than 100-fold greater than uninfected cells (Table 1). Similar levels were detected in cells infected with wild-type EPEC containing the plasmid encoding the truncated EspF′-adenylate cyclase fusion. In contrast, cells infected with mutant EPEC strains containing the full-length fusion plasmid, but with mutations in genes encoding components of either the type III secretion apparatus itself (escN) or the translocation apparatus (espA), did not have elevated cAMP levels. Expression of wild-type EspF does not appear to be required for translocation of EspF as cells infected with an espF mutant expressing the EspF-adenylate cyclase fusion had high levels of cAMP. Similar results were obtained using T84 cells, indicating that EspF is also transported into a relevant intestinal epithelial cell line (Table 1) and in the presence or absence (data not shown) of cytochalasin, which blocks bacterial invasion by greater than 90% (29), indicating that the observed enzyme activity was not due to delivery from internalized bacteria. Western blots using Ab’s directed either against EspF or against adenylate cyclase confirmed that the fusions were expressed by each strain and secreted into the supernatant by all strains except the type III secretion mutant, as expected (data not shown). Overall, these results indicate that the type III secretion system of EPEC delivers EspF to the host cell cytoplasm.
Cyclic AMP levels in HeLa and T84 cells infected with EPEC strains expressing EspF-adenylate cyclase fusion proteins
EspF can be detected within infected host cells by confocal microscopy. To confirm that EspF is translocated into host cells with an alternative method, we used confocal laser scanning microscopy to examine HeLa cells that had been pretreated with cytochalasin D and infected with various EPEC strains. For these studies we stained DNA with DAPI to visualize host cell nuclei and bacteria, EspF with specific antiserum, and host cell membranes with WGA conjugated to Alexa 488. Examination of multiple 0.5-μm optical sections through individual cells clearly demonstrated EspF signal within host cells infected with wild-type EPEC strain E2348/69 (Figure 2). The signals were not homogeneous throughout the infected cells, but were instead present as focal accumulations within the cytoplasm, near but clearly separated from those of the infecting bacteria. In contrast to cells infected with wild-type EPEC, no signal was detected in cells infected with the espF mutant strain UMD874. Weaker EspF signals were detected in samples containing cells infected with escN and espA mutant strains, which have mutations in components of the type III secretion system and the translocation apparatus, respectively. However, the signals in these samples were associated with the bacteria and the cell surface rather than within the host cells. Reconstruction of yz and xz planes through the depth of the epithelial cells confirmed that the EspF signal was within the cells infected with the wild-type strain only, while the signals were near the surface of cells infected with the escN and espA mutant strains (not shown). These studies confirm that the type III secretion and translocation system of EPEC delivers EspF to the interior of host cells.
Confocal laser scanning microscopy of HeLa cells infected with wild-type and mutant EPEC strains. HeLa cells were infected in the presence of cytochalasin D with wild-type EPEC strain E2348/69 (a–d), espF mutant strain UMD874 (e–h), escN mutant strain CVD452 (i–l), and espA mutant strain UMD872 (m–p). Bacterial and cellular nucleic acid was labeled with DAPI (a, e, i, and m), surface carbohydrates were labeled with WGA conjugated to Alexa 488 (b, f, j, and n), and EspF was labeled with affinity-purified EspF antiserum and detected with a secondary Ab against IgG conjugated to lissamine rhodamine (c, g, k, and o). Separate images of the same fields excited at different wavelengths as well as composite images (d, h, l, and p) are shown. In the composite images the bacteria and host cell nuclei appear blue, the cell surface carbohydrates appear green, and EspF appears red.
EspF is required for EPEC-induced loss of transepithelial electrical resistance and increase in paracellular permeability in intestinal epithelial monolayers. Several investigators have reported that EPEC infection of polarized intestinal epithelial cells leads to a loss of TER (18–20). To determine whether EspF plays a role in altering intestinal barrier function, we infected polarized T84 monolayers with EPEC strains at an moi of 500, removed nonadherent organisms as described, and monitored TER over time. This method of infection yields a final ratio of 50 cell-associated bacteria per cell at 6 hours (data not shown). As reported previously, we found a dramatic time-dependent loss of TER in monolayers infected with the wild-type EPEC strain E2348/69 (Figure 3a). However, we detected no significant loss of TER in monolayers infected with espF mutant strain UMD874. The results with the espF mutant were similar to results obtained with the escN type III secretion mutant and the espA translocation mutant, which are incapable of delivering EspF to the host cell (data not shown). In contrast, mutant strain UMD876, which retains espF but has a deletion of the downstream transposon remnants, was able to induce a drop in TER. Furthermore, complementation of strain UMD874 with plasmid pBPM19 containing espF, but no downstream sequences (9), restored the ability of the mutant to induce a drop in TER. In contrast, introduction of pACYC184, the plasmid vector alone, had no effect. Complementation of the escN and espA mutants similarly restored the ability to induce a drop in TER (data not shown). To further evaluate the differences observed between the wild-type and espF mutant strain in ability to induce a drop in TER, we infected T84 cells for 6 hours with these strains at a range of moi. Whereas the wild-type strain induced a significant drop in TER at moi of 250 and greater, the espF mutant strain caused a significant drop in TER only at an moi of 1,000 (Figure 3b). Thus, we conclude that espF is critical for the EPEC-induced drop in TER.
The effect of EPEC strains on intestinal barrier function in T84 monolayers. (a) The TER of polarized T84 monolayers was measured using a simplified apparatus. Monolayers were left uninfected (open circles) or infected at an moi of 500 with wild-type EPEC strain E2348/69 (open squares); espF mutant strain UMD874 (open triangles); strain UMD876, which retains espF but contains a deletion of downstream sequences (filled circles); UMD874 containing plasmid pBPM19, which has the cloned espF gene (filled squares); or UMD874 with control plasmid pACYC184 (filled triangles). TER was measured over time and expressed as a percentage change from baseline values. Data shown represent the means (± SEM) of four or five experiments containing triplicate or quadruplicate samples. The differences between monolayers infected with E2348/69 and UMD874 were significant at 2 hours, 4 hours, and 6 hours (P = 0.006, 0.02, and < 0.001, respectively; Student’s t test). There was no significant difference between monolayers infected with E2348/69 and UMD876 or between those infected with E2348/69 and UMD874 (pBPM19). There was also no significant difference between uninfected monolayers and those infected with UMD874 or UMD874 (pACYC184). The mean ± SEM TER of uninfected monolayers at baseline was 1138 ± 67 ohms·cm2. (b) TER was measured in monolayers infected for 6 hours at the indicated concentrations with wild-type EPEC strain E2348/69 (open squares) or with espF mutant strain UMD874 (open triangles) or left uninfected (open circle). Data shown represent the means (± SEM) of three or four experiments containing triplicate samples. The differences between monolayers infected with E2348/69 and UMD874 were significant at moi of 250 (P = 0.04) and 500 (P < 0.001). (c) Mannitol flux was measured as described in Methods and expressed as a percentage of that recorded in uninfected monolayers. Monolayers were infected for 6 hours with wild-type EPEC strain E2348/69 (open squares) or espF mutant strain UMD874 (open triangles). Data shown represent the means (± SEM) from two experiments containing triplicate samples. The differences between monolayers infected with E2348/69 and UMD874 were significant at moi of 500 (P = 0.02) and 1,000 (P = 0.001).
TER is a highly sensitive method for measuring perturbations in monolayer permeability (36). To determine the physiological significance of the differences in TER observed between the wild-type and espF mutant strains, we measured the flux of mannitol, a compound that diffuses exclusively through the paracellular pathway, in T84 monolayers infected with these strains for 6 hours. We found a dose-dependent increase in mannitol flux that was much more pronounced in monolayers infected with the wild-type EPEC strain than in those infected with the espF mutant strain (Figure 3c). These differences were significant at moi of 500 and 1,000 (corresponding to concentrations of approximately 50 and 100 adherent organisms per cell, respectively, at the end of the incubation period). Thus, we conclude that espF is central to the EPEC-induced increase in paracellular permeability.
To determine whether the differences in TER and mannitol flux induced by the wild-type and espF mutant strains were due to differences in the ability of these strains to cause death of the cells in the monolayer, we measured LDH release at 6 hours. Approximately 2% of total LDH was released both by uninfected cells and by cells infected with the wild-type or the espF mutant at moi up to 500. At an moi of 1,000 the wild-type strain caused a release of 2.1 ± 0.2% of total cellular LDH while the espF mutant strain caused a release of 2.4 ± 0.1% of total cellular LDH (P = 0.2). Thus, the differences between these strains in their ability to disrupt intestinal barrier function are not due to differences in their ability to kill cells.
We noted in these experiments that the mutant strain complemented with the plasmid containing the espF gene consistently induced a greater loss of TER than did the wild-type strain. To determine whether there is a direct relationship between the levels of EspF expressed and the loss of TER, we transformed the espF mutant strain with plasmid pBPM32, which contains the espF gene under control of an inducible trc promoter. We then infected monolayers with this strain in the presence of a range of concentrations of the inducer, IPTG. We found a direct correlation between the concentration of IPTG, the expression of EspF, and the drop in TER (Figure 4). In fact, the correlation coefficient for the relationship between the amount of recombinant EspF expressed (as measured in arbitrary units from the digitized blot image) and the percentage fall in TER was 0.955. Expression of native EspF from wild-type EPEC resulted in a greater loss in TER for the amount of protein expressed than did expression of the recombinant protein in the espF mutant, a difference that we attribute to the altered sequence of the recombinant protein (see Methods), which may have affected its activity. Thus, we conclude that the level of EspF expression is directly related to the drop in TER.
The level of expression of recombinant EspF correlates with ability of EPEC strains to induce a drop in TER in T84 monolayers. Bacteria were grown in the presence or absence of IPTG to induce EspF expression. Cultures were (a) concentrated and examined by immunoblot for EspF expression, or (b) used to infect T84 cells for 4 hours, and the change in TER was determined. Lane 1 in a and the open bar in b represent culture medium alone. Lane 2 and the filled bar represent wild-type EPEC strain E2348/69 in the absence of IPTG. Lanes 3–6 and the gray bars represent espF mutant strain UMD874 containing plasmid pBPM32 grown in the absence (lane 3) and the presence of varying concentrations of IPTG (lanes 4–6), as indicated. Data in b represent the mean (± SEM) of two experiments with triplicate samples. The difference between monolayers infected with UMD874 (pBPM32) in the absence of IPTG and those infected with UMD874 (pBPM32) in the presence of 0.1 mM IPTG was significant (P = 0.04).
EspF is required for EPEC-induced disruption in the distribution of occludin in intestinal epithelial monolayers. To determine whether the observed electrophysiological effect of EspF on intestinal epithelial cell monolayers has a morphological counterpart, we examined the distribution of occludin, a transmembrane tight-junctions protein that contributes to barrier function, in cells infected with EPEC strains. As reported previously (33), the wild-type EPEC strain induces a change in occludin distribution from a uniform band outlining the cell junctions to a discontinuous beaded pattern outlining the cells with increased cytoplasmic staining (Figure 5). In contrast, cells infected with the espF mutant containing the inducible espF gene on pBPM32 in the absence of IPTG retained the uniform pattern of occludin staining. The addition of IPTG to induce expression of EspF from the plasmid caused a dose-dependent relocalization of occludin such that a beaded pattern was apparent at a concentration of 0.01 mM IPTG and a beaded and cytoplasmic pattern similar to that observed in cells infected with the wild-type strain at a concentration of 0.1 mM IPTG.
EspF is required for EPEC-induced changes in the distribution of occludin. T84 cells were left uninfected in the absence (a) or presence (b) of IPTG, infected with wild-type EPEC strain E2348/69 (c), or infected with the espF mutant strain UMD874 containing plasmid pBPM32 in the absence of IPTG (d) or in the presence of 0.01 mM (e) or 0.1 mM (f) IPTG. Occludin was visualized by immunofluorescence using a polyclonal Ab. Note the progressive redistribution of occludin corresponding to the IPTG concentration: a beaded pattern is seen with the lower concentration (e) and a shift from the region of the tight junction to the cytoplasm is seen with the higher concentration (f) similar to that observed with infection by wild-type EPEC (c).