Innate immune cell–epithelial crosstalk during wound repair (original) (raw)
Neutrophils. Neutrophils are the first immune cells to infiltrate wounded tissues, arriving in large numbers in response to DAMPs released from injured and necrotic cells. Murine neutrophil recruitment to wounded tissues begins 4 to 6 hours after initial injury, with maximum numbers detected after 18 to 24 hours (34, 35). The neutrophil’s role in wound healing can be viewed as a double-edged sword (36). Too few neutrophils risks infection and delayed healing (37), whereas overpersistence of neutrophils in injured tissues also delays healing through collateral tissue damage. For example, neutrophils contribute to the crypt loss and ulceration that are pathological hallmarks of ulcerative colitis, and excessive neutrophil infiltration parallels disease severity and patient symptoms (38–40). Therefore, neutrophil activation and migration in response to dermal or mucosal injury is tightly regulated. Impaired leukocyte trafficking delays cutaneous wound healing in mice (41, 42), highlighting neutrophils’ critical importance in orchestrating efficient wound repair. Similarly, neutrophil depletion in damaged intestinal mucosa was associated with increased inflammation, impaired intestinal mucosal repair, and slower recovery from colitis in vivo (43, 44). Furthermore, individuals with neutropenia (or deficiencies in neutrophil trafficking or function) display not only higher risk for developing wound infections but also impaired wound healing (37, 45, 46).
While many previous studies focused on neutrophil trafficking (reviewed elsewhere in refs. 47, 48), the DAMP-triggered mechanisms that facilitate neutrophil migration into skin and intestinal wounds are not yet well described. Once recruited to wounded dermal or intestinal tissues, neutrophils prevent infection by eradicating microbes that enter through disrupted epithelial barriers. Neutrophils destroy invading microbes through phagocytosis, or sometimes NETosis (formation of extracellular traps; ref. 49), while releasing antimicrobial peptides (AMPs, including cathelicidins and β-defensins), ROS, and cytotoxic enzymes such as elastase and myeloperoxidase (Figure 2 and ref. 50). To produce microbicidal ROS, neutrophils consume large amounts of oxygen, generating a hypoxic microenvironment within wounded tissues that results in stabilization of the transcription factor HIF-1α in human and murine intestinal mucosa (51, 52). In wounded intestinal mucosa, HIF-1α stabilization results in enhanced epithelial expression of intestinal trefoil factor (ITF). ITF activates epithelial MAPK signaling and induces reorganization of cell-cell junction proteins including E-cadherin, promoting epithelial mobility and barrier restitution following injury in vitro and in vivo (52, 53). HIF-1α also promotes transcriptional upregulation of genes that enhance cutaneous wound repair, including metabolic proteins, adhesion proteins, soluble growth factors (TGF-β and VEGF), and ECM components (54, 55). Therefore, neutrophil-mediated HIF-1α stabilization in wound microenvironments acts through epithelial cells to promote barrier restitution and a faster return to tissue homeostasis.
Proinflammatory stage of wound healing. Neutrophils are the first responders to epithelial injury. They clear bacteria present at the wound site, limiting infection, and secrete proinflammatory TNF-α, which stimulates fibroblast proliferation and angiogenesis.
In addition to eliminating microbes and modulating the wound microenvironment through oxygen metabolism, neutrophils release pro-repair cytokines, chemokines, and growth factors that signal through wound-associated immune and epithelial cells to promote healing. Following mucosal damage, infiltrating neutrophils secrete TGF-β to activate MEK1/2 signaling and induce intestinal epithelial cell–mediated production of the EGF-like molecule amphiregulin (AREG) (56). AREG promotes intestinal epithelial cell differentiation and proliferation in a positive manner to facilitate efficient return to mucosal homeostasis in vivo (56, 57). TGF-β also accelerates re-epithelization, angiogenesis, and granulation tissue formation in healing murine and human skin wounds (58–60). However, unlike healing intestinal mucosa, neutrophils in skin wounds are not yet identified as an important source of TGF-β. While not implicated in TGF-β production, human neutrophils that migrate into skin wounds upregulate a transcriptional program that includes chemoattractants (e.g., CCL-2 and MIP1α, also known as CCL-3) and genes that promote angiogenesis (VEGF, IL-8, GRO-γ, and CCL-2), proliferation, and activation of keratinocytes and fibroblasts (IL-8, IL-1β, and CCL-2) (61–63). Moreover, several studies reported that neutrophils are an important source of de novo TNF-α synthesis in healing mouse skin lesions (64, 65). While TNF-α is traditionally considered a proinflammatory mediator, it also mediates crucial pro-repair mechanisms, including stimulation of fibroblast proliferation, re-epithelization, and angiogenesis (66).
Neutrophils recruited to wounds also respond to the proinflammatory cytokine–rich milieu by producing CC chemokines such as CCL-20 (67), which attracts CCR-6–expressing inflammatory monocytes into murine injured skin (68). Recent work identified tissue-infiltrating neutrophils as a major source of IL-23 in the intestines of individuals with IBD (69). Furthermore, upon stimulation with IL-23 and TNF-α, murine and human colonic neutrophils produce IL-22, a member of the IL-10 superfamily of cytokines. In murine intestinal wounds, neutrophil-produced IL-22 stimulated intestinal epithelial production of AMPs RegIIIβ and S100A8 and increased epithelial proliferation, differentiation, and migration (70–72). Intestinal injury induces another IL-1 family member, IL-36, in epithelial cells and macrophages, and signaling through IL-36R promotes neutrophil recruitment, IL-22 production, and murine intestinal epithelial repair (73). In murine skin, it is known that IL-22 mediates interactions between immune cells and fibroblasts to promote wound healing (74, 75). However, murine neutrophils have not yet been identified as a prominent source of IL-22 during skin repair.
An additional mechanism whereby neutrophil-epithelial crosstalk promotes mucosal wound healing is via production of chemical mediators, including diadenosine triphosphate (Ap3A). Human colonic epithelial cells metabolize neutrophil-produced Ap3A to adenosine, resulting in downstream activation of surface adenosine receptors and enhanced epithelial barrier function and mucosal wound-healing responses in the gut (76, 77). In the mucosa, neutrophil-derived adenosine signals primarily through cAMP (78), which increases expression of human epithelial tight junction proteins including ZO-1 and occludin and modulates actin and intermediate filament dynamics (79). Like its pro-repair effects in the intestine, topical application of adenosine promotes cutaneous wound healing by stimulating angiogenesis and suppressing inflammatory cell function in mice (80). However, no direct effects of neutrophil-derived adenosine on skin epithelia have been reported to date. Taken together, these studies highlight the range of neutrophil-produced mediators acting on epithelial cells and wound-associated immune cells to promote cutaneous and intestinal wound healing.
The above evidence highlights the dual roles of wound-associated neutrophils that were once thought to simply maintain sterility following injury to include crucial immunomodulatory and pro-resolving or wound-healing functions. Further development of sophisticated imaging methodology including intravital microscopy (81) combined with transgenic strategies that specifically target/label neutrophil subsets in vivo (82) will allow detailed mechanistic analyses of neutrophil behavior within wound-healing environments. Furthermore, recent advances in understanding neutrophil plasticity and identifying distinct neutrophil subsets (83) should be exploited to develop therapeutic strategies to selectively deplete inflammatory neutrophil populations from poorly healing cutaneous and intestinal wounds. Additional studies are needed to identify markers and develop antibodies and small-molecule inhibitors that specifically target inflammatory neutrophils in wounds to promote repair and reduce chronic tissue damage in the skin and gut.
Monocytes. Following the initial neutrophil wound influx, epithelial, endothelial, lamina propria, and infiltrating immune cells release chemokines including CCL-20 and CCL-2 (84). These mediators facilitate subsequent recruitment of circulating monocytes into sites of tissue damage (Figure 2 and ref. 85). Wound-infiltrated monocytes play crucial roles in orchestrating tissue repair, including regulating angiogenesis, clearing cellular debris, and recruiting additional immune cells. Monocytes recruited into wounded tissues further differentiate into macrophages and/or DCs. In murine wounds, chemokines including CCL-2 and CX3CL-1 and their respective receptors, CCR-2 and CX3CR-1, regulate monocyte recruitment. Previous studies show that CX3CR-1 and CCR-2 are essential for wound repair in vivo: _Cx3cr-1_–null mice have delayed healing in skin wounds, and inhibiting CX3CR-1 signaling decreases skin angiogenesis and wound repair (86). In the gut, Ly6Chi monocyte recruitment requires CCR-2, and CCR-2–deficient mice have reduced numbers of monocyte-derived macrophages in wounds (87). Other ligands/receptors involved in monocyte trafficking include CCR-1/CCL-3, CCR-5/CCL-5, CCR-6/CCL-20, CCR-7/CCL-19, and CCR-8/CCL-1 (reviewed in ref. 88).
Interestingly, in mice, some monocytes that leave the circulation to migrate into injured tissues do not differentiate into tissue macrophages or DCs but instead undergo apoptosis and are removed from wound sites. Before removal, these monocytes contribute to wound-healing responses by releasing cytokines and chemokines (88). Tissue-infiltrating monocytes can have inflammatory or antiinflammatory/pro-repair properties. Inflammatory monocytes, typically characterized as Gr1+Ly6Chi, CCR-2+, CX3CR-1lo in mice or CD14+, CD16– in humans, are the major population of mononuclear cells initially recruited to sites of injury (Figure 3). They are a potent source of proinflammatory cytokines such as IL-6 and TNF-α. Shortly after arrival of inflammatory monocytes into wound sites, monocytes with antiinflammatory properties marked by expression of Gr1−Ly6Clo, CCR-2−, CX3CR-1hi in mice and CD14lo, CD16+ in humans are observed. Antiinflammatory monocytes release pro-repair molecules such as VEGF and IL-10, promoting cell proliferation and angiogenesis (Figure 2 and ref. 88). While precise mechanisms are unclear, differential chemoattractant signaling from CCR-2 versus CX3CR-1 may regulate recruitment of proinflammatory versus pro-repair monocytes into murine healing wounds (89). However, it remains unclear whether infiltrated monocytes transition from a proinflammatory to an antiinflammatory phenotype before differentiating into macrophages, or whether independent monocyte populations migrate from the blood and differentiate into these cell types.
Resolution of inflammation and repair. Regenerating epithelial cells express pro-repair molecules including CCL-2, COX2, LGF1, and IL-11, possibly as a result of their activation by TRMs. Wound-associated macrophages (WAMs) and neutrophils also produce pro-repair signals, including annexin A1, VEGF-A, TGF-β, IL-10, and SPMs, that enhance resolution of inflammation at the wound site. In addition to supporting epithelial repair and migration, these pro-repair signals polarize macrophages to M2-like phenotypes that clear apoptotic neutrophils. In the presence of SPMs, neutrophil-derived microparticles may serve as a negative feedback mechanism to suppress additional neutrophil recruitment. TGF-β also stimulates fibroblast differentiation into myofibroblasts, which produce collagen that provides structural support to the healing epithelium.
Both healthy intestine and skin contain resident monocyte-derived macrophages (90). Given that skin and intestinal epithelia are constantly exposed to microorganisms and their products, it follows that there is a dynamically changing population of associated macrophages. Continuous exposure to commensal microorganisms may be viewed as a stimulus that maintains “low-grade” chronic inflammation and induces monocyte recruitment (91). In summary, monocytes migrate to sites of injury (92) and secrete soluble mediators that contribute to wound repair. While many studies focus on macrophage functions in wound healing, the importance of infiltrating monocytes in mediating key aspects of skin and intestinal wound repair remains understudied.
Macrophages. Macrophages contribute to wound repair and tissue remodeling by clearing apoptotic neutrophils (efferocytosis) and helping to reduce autoimmune and chronic inflammatory responses (92). These effector functions are achieved, in part, through secretion of cytokines, growth factors, and specialized pro-resolving mediators (SPMs) (93). Wound-associated macrophages undergo polarization, a process involving integration of complex signals from the microenvironment followed by commitment to a functional program directed at restoring tissue homeostasis. Polarization continuously changes throughout the phases of wound healing. Historically, macrophage characterization was based on M1 (inflammatory) or M2 (antiinflammatory/pro-repair) phenotypes. M1 macrophages are induced by inflammatory stimuli such as lipopolysaccharide and, when stimulated, release proinflammatory cytokines such as TNF-α, IL-1β, IL-6, IL-8, and IL-12. In contrast, M2 macrophages are induced by IL-4/IL-13 as well as IL-10. M2 macrophages release antiinflammatory/pro-repair molecules including TGF-β, IL-10, and SPMs such as maresins, resolvins, and protectins (see ref. 94 for a detailed review on SPMs, and refs. 95, 96). It is increasingly appreciated that M1/M2 macrophage classification is oversimplified; substantial overlap in the responses of these two types of macrophages is undoubtedly due to intermediate or transitional stages of differentiation. In support of such “plasticity” in tissue macrophage responses, M1-type macrophages were shown to switch to an M2 phenotype depending on the composition of the local extracellular milieu (97). Furthermore, M1/M2 macrophage classification is largely based on in vitro differentiation, and increasing evidence suggests that such in vitro analyses do not accurately reflect the complexity of in vivo macrophage plasticity and heterogeneity. Adding to the complexity of macrophage classification, current evidence also suggests that monocytes that have entered tissues differentiate into macrophages displaying varying M1- or M2-like characteristics (98). These observations and emerging evidence increasingly imply that diverse macrophage populations mediate healing responses by releasing cytokines/chemokines, SPMs, proteases, and other mediators to orchestrate host defense, proliferation, and migration of wound-associated cells as well as matrix remodeling. Further studies are needed to better understand the role(s) of specific tissue macrophage subsets during the stages of wound repair (99).
Most tissues (including skin and intestine) contain macrophages termed tissue-resident macrophages (TRMs). TRMs represent a heterogeneous population of nonmigratory cells that respond to injury or infection by sensing DAMPs. TRMs in the skin and gut are continuously replenished by blood monocytes (100–102). TRM phenotypic markers vary depending on tissue location. In skin, they have surface expression of F4/80+, CD11b+, CD11clo, CD206+, MHCIIlo, Dectin-1+, CD301+, and Dectin-2+, while in intestine, their cell surface markers include CX3CR-1hi, F4/80+, CD11b+, CD11c+, and CD64+. Intestinal (and a subset of dermal) MHCIIhi macrophages have been shown to originate from bone marrow monocytes. These macrophages lack capacity for self-renewal and have an estimated half-life of 4 to 6 weeks (103). An interesting subset of resident macrophages are epidermal Langerhans cells. While Langerhans cells were historically considered DCs, they are now believed to represent a specialized subset of TRMs. Unlike dermal TRMs, Langerhans cells are self-replicating and can migrate to lymph nodes to present antigens to T cells (104). Importantly, Langerhans cells were reported to repopulate the epidermis during re-epithelialization of acute skin wounds (105). Despite these observations, mechanisms regulating TRM-mediated wound repair are poorly understood, although increasing reports implicate contributions of dermal TRMs to homeostatic maintenance, renewal of skin appendages, epithelial repair, and barrier recovery (106, 107). Important functions of intestinal TRMs include scavenging bacteria, helping maintain Tregs, and promoting epithelial cell renewal via production of IL-10 and prostaglandin E2 (89, 100, 108, 109). Taken together, current evidence supports an emerging concept of multiple roles for TRMs, including maintenance of homeostasis in epithelial tissues as well as facilitating inflammatory responses and mediating repair following injury.
Macrophages in sites of injury are critical for skin and gut wound repair. Such wound-associated macrophages (WAMs) are adaptive, highly dynamic cells that can rapidly respond to cues within wound microenvironments (Figure 3). Current observations indicate that WAM phenotype is influenced by complex factors that are incompletely understood, including wound size, tissue location, and stage of the inflammatory process (acute versus chronic). Like other macrophages, WAMs can have varied M1/M2 phenotypes depending on the inflammatory/repair microenvironment, exhibiting characteristics of both proinflammatory and pro-repair macrophages (89). Given these observations, it is unsurprising that current literature is inconsistent on the types of cytokines produced by WAMs. Some studies report that WAMs are not an important source of the antiinflammatory cytokine IL-10 in skin wounds, whereas other groups demonstrate that both skin and gut WAMs are active producers of IL-10 with pro-repair properties (110, 111). Such discrepancies are likely related to the temporal nature of IL-10–dependent responses during wound repair. Supporting this notion, analyses of WAM-mediated IL-10 production suggest that IL-10 is produced at very specific times during the wound repair process, indicating that variables including wound size profoundly influence macrophage cytokine production responses.
Macrophages and other immune cells sense the metabolic environment and modulate function, an activity referred to as immunometabolism (112). Sites of injury have a hypoxic microenvironment generated primarily by neutrophils consuming high levels of oxygen while producing ROS in response to injury (113). Hypoxia also promotes increased HIF-1α expression in inflammatory macrophages, which increases glycolytic enzyme expression and IL-1β synthesis (114). Balanced IL-1β release is important, as excess inflammasome signaling associated with IL-1β generation is linked to development of chronic wounds (115). Phagocytosis of cellular debris in association with IL-4 and IL-13 signaling facilitates dampening of inflammatory signals and initiation of the proliferative phase of tissue repair (116). Glucose is an important source of energy for inflammatory macrophage–mediated clearance of cellular debris that influences the proliferative phase in wound repair. Importantly, glucose availability likely influences macrophage secretion of proinflammatory mediators such as IL-1β and TNF-α (117). Interestingly, pro-repair macrophages have a highly oxidative metabolism, and therefore restoring oxygen levels is important in achieving resolution of inflammation (118).
Regenerative responses are likely mediated, in part, by intimate physical contact between macrophages and epithelial cells that promotes intestinal epithelial transcription of multiple pro-repair genes, including Ccl-2, Cox-2, Igf-1, and Il-11 (Figure 3). Furthermore, since Cox-2 (encoding cyclooxygenase-2) is necessary for SPM synthesis, macrophage-mediated “activation” of epithelial cells might contribute to the generation of SPMs (119). Current evidence suggests that intestinal WAMs are required for amplification of colonic epithelial cell progenitors that contribute to wound repair. WAMs physically contact epithelial stem cells located within crypts, resulting in secretion of pro-proliferative and remodeling factors. Furthermore, recent evidence indicates that intestinal macrophages promote regenerative responses by integrating cues from mesenchymal stem cells, other immune cells, microbiota, and injured epithelia. Efficient colonic wound repair also depends on Trem2 signaling in WAMs, which skews cellular machinery toward a pro-repair phenotype (119, 120). CD206+CD301b+ skin macrophages also produce the crucial pro-repair molecule TGF-β1, a potent inducer of fibroblast proliferation and subsequent differentiation into myofibroblasts, leading to collagen deposition in the wound (121–125). WAMs also promote epithelial repair through release of IL-10 and PDGF-β (125).
Macrophages can also directly transition into fibrosis-promoting cells, secreting ECM components such as collagen (126). These macrophages, referred to as fibrocytes or M2a macrophages, are implicated in pathogenesis of skin scarring. Interactions between macrophages and fibroblasts are critical in determining whether wounds heal with or without scarring. Regulatory-like, or M2c, macrophages within remodeling skin wounds release proteases and phagocytose cellular debris and ECM to clean out wounds and facilitate repair (127). In skin, WAMs are hypothesized to synthesize several members of the EGF family, e.g., EGF, TGF-α, and heparin-bound EGF (EGF-HB), which enhance keratinocyte migration and proliferation, thereby promoting skin re-epithelialization (128–132). Inactive EGF family members are tethered to the cell membrane and require MMP-mediated cleavage to signal. Therefore, WAMs likely indirectly activate these growth factors by modulating MMP activity. IL-1, IL-6, TNF-α, and TGF-β also promote re-epithelialization (133). Interestingly, in human keratinocytes, WAM-derived TNF-α promotes expression of genes associated with cell movement, division, and survival (134). Presently, no studies highlight contributions of M2a and M2c macrophages to repair in the intestine. Recent observations indicate that WAMs in close proximity to wounded dermal and intestinal epithelial cells (and underlying fibroblasts) play important roles in orchestrating matrix remodeling and wound repair. As such, aberrations in macrophage function at different stages of wound repair markedly contribute to persistence of excessive ECM, resulting in skin fibrosis and permanent scarring.
Recently, further insights into the role of macrophages in wound repair have been gained from mouse models using depleted subsets of macrophages. Mice lacking the Spi-1 proto-oncogene protein lack mature macrophages as well as functional neutrophils. Surprisingly, these mice lack a skin wound-healing defect but rather exhibit marked reduction in scar formation (135). In support of macrophages’ critical importance in skin wound repair, ablation of macrophages impaired murine skin wound healing (136, 137). These studies support an important role of macrophages in removing apoptotic neutrophils from wounds, thereby preventing ongoing release of tissue-degrading enzymes. Furthermore, when macrophages fail to appropriately clear apoptotic neutrophils, there are persistently high levels of proinflammatory cytokines and decreased local antiinflammatory and pro-repair mediators in wounds (138–140). Depletion of macrophages was also shown to reduce myofibroblast differentiation, which is necessary to promote wound contraction and accelerate skin wound healing (141).
Genetically engineered and inducible depletion models in mice enable selective macrophage depletion at different stages of the healing process, providing insights into the role of macrophages at various stages of wound repair. Macrophage depletion at early and mid-stages of skin repair results in delayed wound closure and decreased scar formation, while macrophage loss during later stages of repair did not affect healing. Depletion of macrophages at mid-stages of skin wound repair resulted in decreased VEGF-A and TGF-β1 expression, as well as reduced angiogenesis and repair. Consistent with these observations, it was noted that during mid-stages of repair, macrophages secrete substantial amounts of VEGF-A and TGF-β1 (106). The above-mentioned mouse models have not yet been used to study the role of macrophages in orchestrating intestinal mucosal repair in vivo. However, analogous temporal changes in macrophage function are likely necessary for mucosal repair in the gut. These findings highlight macrophages as critical to epithelial wound repair, displaying a dynamic capacity to polarize in response to environmental cues that change as wound healing progresses.
While we have discussed contribution of macrophages in orchestrating wound repair, DCs are also implicated as important innate mediators of repair. This topic is discussed in previous publications and reviews (142–144).
Therapeutic opportunities. Several studies have either targeted neutrophils/macrophages or used these cells as tools as part of strategies to improve wound healing. Nevertheless, such therapeutic targeting of innate immune cells has been limited by incomplete understanding of underlying mechanisms by which these cell populations regulate repair. Early research focusing on promoting neutrophil apoptosis yielded promising results, but off-target cell death presented a challenge (145). Novel technologies and drugs aided in the development of new strategies to promote wound repair by inducing resolution of inflammation without reducing neutrophil recruitment. A recent study observed that neutrophils “retrotax,” or reverse-migrate, away from inflammatory sites when exposed to SPMs (146). Manipulating this process could potentially improve healing as well as infection control. Other studies showed potential “therapeutic benefit” through controlled delivery of leukocyte-derived SPMs. For example, nanoparticles containing neutrophil-derived microparticles with aspirin-triggered resolvin D1 or lipoxin A4 analogs reduced neutrophil recruitment in murine peritonitis and accelerated keratinocyte wound healing (147).
Strategies to improve wound healing through increased macrophage recruitment and polarization toward a pro-repair phenotype have also been investigated. Direct injection of IL-1β–activated macrophages into murine skin wounds increased VEGF-C production and improved wound repair (148). Furthermore, local GM-CSF application to dermal wounds resulted in increased WAMs and enhanced wound healing (149). Since the complex biomolecular microenvironment within wounds plays a critical role in regulating macrophage polarization, strategies to enhance production or delivery of pro-repair molecules have been explored. For example, glutamine-loaded hydrogels increased the rate of wound closure and re-epithelialization in wounded skin. In this study, collagen deposition within wounds was consistent with increased activity of alternatively activated macrophages (150). Conversely, strategies targeting inhibition of alternative macrophage activation and resulting Arg-1 activity may help prevent scarring and fibrosis by reducing excessive collagen deposition (151). From these observations, it is clear that methods promoting macrophage activity or polarization to a wound-healing phenotype have considerable promise, further supported by multiple other reports employing mesenchymal stem cells, growth factors, and biomaterials to modulate macrophage phenotype, function, and transcriptome (152–154).