Antibody-mediated blockade of the CXCR3 chemokine receptor results in diminished recruitment of T helper 1 cells into sites of inflammation (original) (raw)
Journal Article
,
Department of Pharmacology, Merck Research Laboratories
,
Rahway, New Jersey
Correspondence: Dr. Jenny H. Xie, R80Y-125, Merck Research Laboratories, P.O. Box 2000, Rahway, NJ 07065. E-mail: Jenny_xie@merck.com
Search for other works by this author on:
,
Department of Pharmacology, Merck Research Laboratories
,
Rahway, New Jersey
Search for other works by this author on:
,
Department of Immunology & Rheumatology, Merck Research Laboratories
,
Rahway, New Jersey
Search for other works by this author on:
,
Department of Immunology & Rheumatology, Merck Research Laboratories
,
Rahway, New Jersey
Search for other works by this author on:
,
Department of Immunology & Rheumatology, Merck Research Laboratories
,
Rahway, New Jersey
Search for other works by this author on:
,
Department of Immunology & Rheumatology, Merck Research Laboratories
,
Rahway, New Jersey
Search for other works by this author on:
,
Department of Pharmacology, Merck Research Laboratories
,
Rahway, New Jersey
Search for other works by this author on:
,
Department of Immunology & Rheumatology, Merck Research Laboratories
,
Rahway, New Jersey
Search for other works by this author on:
,
Department of Immunology & Rheumatology, Merck Research Laboratories
,
Rahway, New Jersey
Search for other works by this author on:
,
Department of Pharmacology, Merck Research Laboratories
,
Rahway, New Jersey
Search for other works by this author on:
Received:
20 November 2002
Revision received:
27 February 2003
Accepted:
28 February 2003
Cite
Jenny H Xie, Naomi Nomura, Min Lu, Shiow-Ling Chen, Greg E Koch, Youmin Weng, Raymond Rosa, Jerry Di Salvo, John Mudgett, Laurence B Peterson, Linda S Wicker, Julie A DeMartino, Antibody-mediated blockade of the CXCR3 chemokine receptor results in diminished recruitment of T helper 1 cells into sites of inflammation, Journal of Leukocyte Biology, Volume 73, Issue 6, June 2003, Pages 771–780, https://doi.org/10.1189/jlb.1102573
Close
Navbar Search Filter Mobile Enter search term Search
Abstract
Naïve T cells, when activated by specific antigen and cytokines, up-regulate adhesion molecules as well as chemokine receptors on their surface, which allows them to migrate to inflamed tissues. Human studies have shown that CXCR3 is one of the chemokine receptors that is induced during T cell activation. Moreover, CXCR3-positive T cells are enriched at inflammatory sites in patients with autoimmune diseases such as rheumatoid arthritis and multiple sclerosis. In this study, we use a mouse model of inflammation to demonstrate that CXCR3 is required for activated T cell transmigration to inflamed tissue. Using an anti- mCXCR3 antibody, we have shown that in vitro-differentiated T helper (Th) 1 and Th2 cells up-regulated CXCR3 upon stimulation with specific antigen/major histocompatibility complex. However, only Th1 cells, when adoptively transferred to syngeneic recipients, are efficiently recruited to the peritoneum in an adjuvant-induced peritonitis model. Furthermore, the neutralizing anti-mCXCR3 antibody profoundly inhibits the recruitment of Th1 cells to the inflamed peritoneum. Real-time, quantitative reverse transcriptase-polymerase chain reaction analysis demonstrates that the CXCR3 ligands, interferon (IFN)-inducible protein 10 (CXCL10) and IFN-inducible T cell α chemoattractant (CXCL11), are among the many chemokines induced in the adjuvant-treated peritoneum. The anti-mCXCR3 antibody is also effective in inhibiting a delayed-type hypersensitivity response, which is largely mediated by enhanced trafficking of activated T cells to peripheral inflammatory sites. Collectively, our results suggest that CXCR3 has a critical role in T cell transmigration to sites of inflammation and thus, may serve as a molecular target for anti-inflammatory therapies.
© 2003 Society for Leukocyte Biology
You do not currently have access to this article.
Personal account
- Sign in with email/username & password
- Get email alerts
- Save searches
- Purchase content
- Activate your purchase/trial code
- Add your ORCID iD
Get help with access
Institutional access
Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:
IP based access
Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.
Sign in through your institution
Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.
- Click Sign in through your institution.
- Select your institution from the list provided, which will take you to your institution's website to sign in.
- When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
- Following successful sign in, you will be returned to Oxford Academic.
If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.
Sign in with a library card
Enter your library card number to sign in. If you cannot sign in, please contact your librarian.
Society Members
Society member access to a journal is achieved in one of the following ways:
Sign in through society site
Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:
- Click Sign in through society site.
- When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
- Following successful sign in, you will be returned to Oxford Academic.
If you do not have a society account or have forgotten your username or password, please contact your society.
Sign in using a personal account
Some societies use Oxford Academic personal accounts to provide access to their members. See below.
Personal account
A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.
Some societies use Oxford Academic personal accounts to provide access to their members.
Viewing your signed in accounts
Click the account icon in the top right to:
- View your signed in personal account and access account management features.
- View the institutional accounts that are providing access.
Signed in but can't access content
Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.
Institutional account management
For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.
Purchase
Short-term Access
To purchase short-term access, please sign in to your personal account above.
Don't already have a personal account? Register
Antibody-mediated blockade of the CXCR3 chemokine receptor results in diminished recruitment of T helper 1 cells into sites of inflammation - 24 Hours access
EUR €48.00
GBP £41.00
USD $51.00
Rental
This article is also available for rental through DeepDyve.
Citations
Views
Altmetric
Metrics
Total Views 184
127 Pageviews
57 PDF Downloads
Since 2/1/2023
| Month: | Total Views: |
|---|---|
| February 2023 | 6 |
| March 2023 | 29 |
| April 2023 | 11 |
| May 2023 | 3 |
| June 2023 | 4 |
| July 2023 | 6 |
| August 2023 | 11 |
| September 2023 | 5 |
| October 2023 | 13 |
| November 2023 | 6 |
| December 2023 | 3 |
| January 2024 | 9 |
| February 2024 | 15 |
| March 2024 | 5 |
| April 2024 | 6 |
| May 2024 | 7 |
| June 2024 | 7 |
| July 2024 | 7 |
| August 2024 | 7 |
| September 2024 | 10 |
| October 2024 | 14 |
Citations
133 Web of Science
×
Email alerts
Citing articles via
More from Oxford Academic