Osteoclast-Like Cell Formation and its Regulation by Osteotropic Hormones in Mouse Bone Marrow Cultures* (original) (raw)

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Department of Biochemistry and Oral Pathology (N.T., H. Y., S. Y., T.S.), School of Dentistry, Showa University

Shinagawa-ku, Tokyo 142, Japan

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Medical Service of Veterans Administration Hospital (G.D.R.) and Department of Medicine (G.D.R., G.R.M.), University of Texas Health Science Center

San Antonio, Texas 78284

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Department of Anatomy and Embryology (S.J.J., A.B.), University College London

London, WClE 6BT, United Kingdom

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Department of Biochemistry and Oral Pathology (N.T., H. Y., S. Y., T.S.), School of Dentistry, Showa University

Shinagawa-ku, Tokyo 142, Japan

;

Medical Service of Veterans Administration Hospital (G.D.R.) and Department of Medicine (G.D.R., G.R.M.), University of Texas Health Science Center

San Antonio, Texas 78284

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Department of Anatomy and Embryology (S.J.J., A.B.), University College London

London, WClE 6BT, United Kingdom

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Department of Biochemistry and Oral Pathology (N.T., H. Y., S. Y., T.S.), School of Dentistry, Showa University

Shinagawa-ku, Tokyo 142, Japan

;

Medical Service of Veterans Administration Hospital (G.D.R.) and Department of Medicine (G.D.R., G.R.M.), University of Texas Health Science Center

San Antonio, Texas 78284

;

Department of Anatomy and Embryology (S.J.J., A.B.), University College London

London, WClE 6BT, United Kingdom

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Department of Biochemistry and Oral Pathology (N.T., H. Y., S. Y., T.S.), School of Dentistry, Showa University

Shinagawa-ku, Tokyo 142, Japan

;

Medical Service of Veterans Administration Hospital (G.D.R.) and Department of Medicine (G.D.R., G.R.M.), University of Texas Health Science Center

San Antonio, Texas 78284

;

Department of Anatomy and Embryology (S.J.J., A.B.), University College London

London, WClE 6BT, United Kingdom

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Department of Biochemistry and Oral Pathology (N.T., H. Y., S. Y., T.S.), School of Dentistry, Showa University

Shinagawa-ku, Tokyo 142, Japan

;

Medical Service of Veterans Administration Hospital (G.D.R.) and Department of Medicine (G.D.R., G.R.M.), University of Texas Health Science Center

San Antonio, Texas 78284

;

Department of Anatomy and Embryology (S.J.J., A.B.), University College London

London, WClE 6BT, United Kingdom

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Department of Biochemistry and Oral Pathology (N.T., H. Y., S. Y., T.S.), School of Dentistry, Showa University

Shinagawa-ku, Tokyo 142, Japan

;

Medical Service of Veterans Administration Hospital (G.D.R.) and Department of Medicine (G.D.R., G.R.M.), University of Texas Health Science Center

San Antonio, Texas 78284

;

Department of Anatomy and Embryology (S.J.J., A.B.), University College London

London, WClE 6BT, United Kingdom

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Department of Biochemistry and Oral Pathology (N.T., H. Y., S. Y., T.S.), School of Dentistry, Showa University

Shinagawa-ku, Tokyo 142, Japan

;

Medical Service of Veterans Administration Hospital (G.D.R.) and Department of Medicine (G.D.R., G.R.M.), University of Texas Health Science Center

San Antonio, Texas 78284

;

Department of Anatomy and Embryology (S.J.J., A.B.), University College London

London, WClE 6BT, United Kingdom

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Department of Biochemistry and Oral Pathology (N.T., H. Y., S. Y., T.S.), School of Dentistry, Showa University

Shinagawa-ku, Tokyo 142, Japan

;

Medical Service of Veterans Administration Hospital (G.D.R.) and Department of Medicine (G.D.R., G.R.M.), University of Texas Health Science Center

San Antonio, Texas 78284

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Department of Anatomy and Embryology (S.J.J., A.B.), University College London

London, WClE 6BT, United Kingdom

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NAOYUKI TAKAHASHI, HIROMI YAMANA, SHUSAKU YOSHIKI, G. DAVID ROODMAN, GREGORY R. MUNDY, SHEILA J. JONES, ALAN BOYDE, TATSUO SUDA, Osteoclast-Like Cell Formation and its Regulation by Osteotropic Hormones in Mouse Bone Marrow Cultures, Endocrinology, Volume 122, Issue 4, 1 April 1988, Pages 1373–1382, https://doi.org/10.1210/endo-122-4-1373
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We developed a mouse bone marrow culture system to examine the process of osteoclast-like multinucleated cell formation from its progenitors. When mouse marrow cells were cultured for 8 days with la,25-dihydroxyvitamin D3 [1α,25- (OH)2D3, 10-10 to 10-7 M] or human PTH (1-34) (25–100 ng/ml), tartrate-resistant acid phosphatase (TRACP)-positive multinucleated cells formed. No TRACP-positive multinucleated cells appeared in the absence of these hormones. 1α,25-(OH)2D3 and PTH also increased the number of the clusters of TRACPpositive mononuclear cells. Time course studies showed that these TRACP-positive mononuclear cell clusters appeared before the formation of TRACP-positive multinucleated cells, suggesting that the TRACP-positive mononuclear cells are precursors of the multinucleated cells. Salmon calcitonin markedly inhibited the formation of TRACP-positive multinucleated cells but not TRACP-positive mononuclear cell clusters induced by 1α,25-(OH)2D3 or PTH. TRACP-positive mononuclear cells and multinucleated cells were rarely stained for nonspecific esterase, but some mononuclear cells were positively stained for both nonspecific esterase and TRACP. More that 90% of the TRACPpositive mononuclear cell clusters and multinucleated cells were found near colonies of alkaline phosphatase-positive mononuclear cells (possibly osteoblasts). When marrow mononuclear cells were cultured on sperm whale dentine slices in the presence of 1α,25-(OH)2D3 or PTH, numerous resorption lacunae were formed. These results suggest that 1) TRACP-positive multinucleated cells formed in response to osteotropic hormones in mouse marrow cultures satisfy most of the criteria of osteoclasts, and 2) osteoblasts may play an important role in osteoclast formation. (Endocrinology122: 1373–1382, 1988)

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