Changes in Basal and Insulin and Amino Acid Response of Whole Body and Skeletal Muscle Proteins in Obese Men (original) (raw)

Journal Article

,

1Université Clermont 1 (C.Gu., Y.B.), Unité de Formation et de Recherche Médecine, Unité Mixte de Recherche (UMR) 1019, Centre de Recherche en Nutrition Humaine-Auvergne, Clermont-Ferrand, F-63009 France

Search for other works by this author on:

,

2Centre Hospitalier Universitaire Clermont-Ferrand (I.D., Y.B.), Hospital Gabriel Montpied, Clinical Nutrition Unit, Clermont-Ferrand, F-63001 France

Search for other works by this author on:

,

3Laboratory of Exercise Biology (M.R., M.B., P.D.), Auvergne and Blaise-Pascal University, Clermont-Ferrand, F-63000 France

Search for other works by this author on:

,

4Institut National de la Recherche Agronomique, UMR 1019 (C.Gi., S.W., Y.B.), Clermont-Ferrand, F-63009 France

*Address all correspondence and requests for reprints to: Christelle Guillet, Ph.D., Univ Clermont 1, Unité de Formation et de Recherche Médecine, Unité Mixte de Recherche 1019, Laboratoire de Nutrition Humaine, BP 321, 58 rue Montalembert, 63009 Clermont-Ferrand cedex 1, France.

Search for other works by this author on:

,

4Institut National de la Recherche Agronomique, UMR 1019 (C.Gi., S.W., Y.B.), Clermont-Ferrand, F-63009 France

Search for other works by this author on:

,

3Laboratory of Exercise Biology (M.R., M.B., P.D.), Auvergne and Blaise-Pascal University, Clermont-Ferrand, F-63000 France

Search for other works by this author on:

,

3Laboratory of Exercise Biology (M.R., M.B., P.D.), Auvergne and Blaise-Pascal University, Clermont-Ferrand, F-63000 France

Search for other works by this author on:

1Université Clermont 1 (C.Gu., Y.B.), Unité de Formation et de Recherche Médecine, Unité Mixte de Recherche (UMR) 1019, Centre de Recherche en Nutrition Humaine-Auvergne, Clermont-Ferrand, F-63009 France

2Centre Hospitalier Universitaire Clermont-Ferrand (I.D., Y.B.), Hospital Gabriel Montpied, Clinical Nutrition Unit, Clermont-Ferrand, F-63001 France

4Institut National de la Recherche Agronomique, UMR 1019 (C.Gi., S.W., Y.B.), Clermont-Ferrand, F-63009 France

Search for other works by this author on:

Received:

10 October 2008

Published:

01 August 2009

• • Article contents
  • Figures & tables
  • Video
  • Audio
  • Supplementary Data
• Cite

Cite

Christelle Guillet, Ingrid Delcourt, Melanie Rance, Christophe Giraudet, Stephane Walrand, Mario Bedu, Pascale Duche, Yves Boirie, Changes in Basal and Insulin and Amino Acid Response of Whole Body and Skeletal Muscle Proteins in Obese Men, The Journal of Clinical Endocrinology & Metabolism, Volume 94, Issue 8, 1 August 2009, Pages 3044–3050, https://doi.org/10.1210/jc.2008-2216
Close

Navbar Search Filter Mobile Enter search term Search

Context: Obesity-related insulin resistance of glucose and lipid metabolism may also affect protein kinetics, notably at the muscle level.

Objective: We hypothesized that muscle protein response to insulin and amino acid is blunted during obesity.

Research Design and Methods: Total (Tot) and mitochondrial (Mit) muscle proteins fractional synthesis rates (FSR) together with whole-body protein kinetics (WB) have been determined in postabsorptive state (PA) and during a hyperinsulinemic, hyperaminoacidemic, euglycemic clamp by using a continuous infusion of 13C-leucine in six obese and eight nonobese subjects.

Results: Responses of WB glucose disposal rate and protein breakdown to insulin and amino acid infusion were significantly lower in obese than in nonobese subjects (P < 0.05). In PA, Tot and Mit FSR were significantly lower (P < 0.05) in obese (Tot, 0.044 ± 0.005% · h−1; Mit, 0.064 ± 0.008% · h−1) in comparison with nonobese subjects (Tot, 0.082 ± 0.010% · h−1; Mit, 0.140 ± 0.006% · h−1). Tot FSR was similarly stimulated by insulin and amino acid in both groups (0.094 ± 0.013 vs. 0.117 ± 0.006% · h−1, obese vs. nonobese; P < 0.05). Mit FSR was increased in nonobese subjects (0.179 ± 0.007% · h−1; P < 0.05) but not in obese subjects (0.078 ± 0.012% · h−1; P = not significant).

Conclusions: The obesity-related impairment of protein metabolism is characterized by 1) a reduced turnover rate of skeletal muscle proteins in PA; 2) a lack of stimulation of mitochondrial protein synthesis by insulin and amino acid; and 3) a lower inhibition of WB proteolysis by insulin and amino acid. Alterations of selective muscle protein kinetics may predispose obese subjects to muscle metabolic dysfunction leading to type 2 diabetes.

Copyright © 2009 by The Endocrine Society

You do not currently have access to this article.

Personal account

• Sign in with email/username & password
• Get email alerts
• Save searches
• Purchase content
• Activate your purchase/trial code
• Add your ORCID iD

Get help with access

Institutional access

Access to content on Oxford Academic is often provided through institutional subscriptions and purchases. If you are a member of an institution with an active account, you may be able to access content in one of the following ways:

IP based access

Typically, access is provided across an institutional network to a range of IP addresses. This authentication occurs automatically, and it is not possible to sign out of an IP authenticated account.

Sign in through your institution

Choose this option to get remote access when outside your institution. Shibboleth/Open Athens technology is used to provide single sign-on between your institution’s website and Oxford Academic.

1. Click Sign in through your institution.
2. Select your institution from the list provided, which will take you to your institution's website to sign in.
3. When on the institution site, please use the credentials provided by your institution. Do not use an Oxford Academic personal account.
4. Following successful sign in, you will be returned to Oxford Academic.

If your institution is not listed or you cannot sign in to your institution’s website, please contact your librarian or administrator.

Sign in with a library card

Enter your library card number to sign in. If you cannot sign in, please contact your librarian.

Society Members

Society member access to a journal is achieved in one of the following ways:

Sign in through society site

Many societies offer single sign-on between the society website and Oxford Academic. If you see ‘Sign in through society site’ in the sign in pane within a journal:

1. Click Sign in through society site.
2. When on the society site, please use the credentials provided by that society. Do not use an Oxford Academic personal account.
3. Following successful sign in, you will be returned to Oxford Academic.

If you do not have a society account or have forgotten your username or password, please contact your society.

Sign in using a personal account

Some societies use Oxford Academic personal accounts to provide access to their members. See below.

Personal account

A personal account can be used to get email alerts, save searches, purchase content, and activate subscriptions.

Some societies use Oxford Academic personal accounts to provide access to their members.

Viewing your signed in accounts

Click the account icon in the top right to:

• View your signed in personal account and access account management features.
• View the institutional accounts that are providing access.

Signed in but can't access content

Oxford Academic is home to a wide variety of products. The institutional subscription may not cover the content that you are trying to access. If you believe you should have access to that content, please contact your librarian.

Institutional account management

For librarians and administrators, your personal account also provides access to institutional account management. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.

Purchase

Short-term Access

To purchase short-term access, please sign in to your personal account above.

Don't already have a personal account? Register

Changes in Basal and Insulin and Amino Acid Response of Whole Body and Skeletal Muscle Proteins in Obese Men - 24 Hours access

EUR €38.00

GBP £33.00

USD $41.00

Rental

This article is also available for rental through DeepDyve.

Citations

Views

Altmetric

Metrics

Total Views 1,928

1,323 Pageviews

605 PDF Downloads

Since 2/1/2017

Citations

135 Web of Science

×

Email alerts

More on this topic

Related articles in PubMed

Citing articles via

• Latest

• Most Read

• Most Cited

More from Oxford Academic