Islet Number Rather Than Islet Size Is a Major Determinant of β- and α-Cell Mass in Humans (original) (raw)

Journal Article

Kinsei Kou ,

1Departments of Internal Medicine (K.K., Y.S., S.S., H.I.), Keio University School of Medicine, Tokyo 160-8582, Japan

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Yoshifumi Saisho ,

1Departments of Internal Medicine (K.K., Y.S., S.S., H.I.), Keio University School of Medicine, Tokyo 160-8582, Japan

*Address all correspondence and requests for reprints to: Yoshifumi Saisho, MD, PhD, Department of Internal Medicine, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.

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Seiji Sato ,

1Departments of Internal Medicine (K.K., Y.S., S.S., H.I.), Keio University School of Medicine, Tokyo 160-8582, Japan

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Taketo Yamada ,

2Pathology (T.Y.), Keio University School of Medicine, Tokyo 160-8582, Japan

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Hiroshi Itoh

1Departments of Internal Medicine (K.K., Y.S., S.S., H.I.), Keio University School of Medicine, Tokyo 160-8582, Japan

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Received:

09 October 2013

Accepted:

28 January 2014

Cite

Kinsei Kou, Yoshifumi Saisho, Seiji Sato, Taketo Yamada, Hiroshi Itoh, Islet Number Rather Than Islet Size Is a Major Determinant of β- and α-Cell Mass in Humans, The Journal of Clinical Endocrinology & Metabolism, Volume 99, Issue 5, 1 May 2014, Pages 1733–1740, https://doi.org/10.1210/jc.2013-3731
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Objective:

The objective of the study was to clarify the relative contribution of islet number and islet size to β- and α-cell mass in humans.

Research Design and Methods:

We obtained the pancreas at autopsy from 72 Japanese adults with no history of diabetes or pancreatitis (aged 47 ± 12 years, body mass index 24.1 ± 5.0 kg/m2). Pancreatic sections were stained for insulin or glucagon, and fractional β-cell area (%BCA) and α-cell area (%ACA) were measured. Islet number and islet size as well as β-cell turnover were also quantified. Glycosylated hemoglobin measured within 1 year prior to death was obtained in 38 individuals.

Results:

There was considerable interindividual variation in islet density and mean islet size, with a significant negative correlation between the two (r = −0.25, P = .03). There were significant positive correlations between islet density and %BCA or %ACA (r = 0.63, P < .001, and r = 0.41, P = .001), whereas mean islet size correlated with neither of them. Islet density as well as %BCA, but not mean islet size, was negatively correlated with glycosylated hemoglobin (r = −0.37, P = .02, and r = −0.36, P = .03).

Conclusion:

The present study suggests that islet number rather than islet size is a major determinant of β- and α-cell mass in humans. Interindividual difference in islet number may contribute to susceptibility to development of glucose intolerance.

Copyright © 2014 by the Endocrine Society

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