The Human Androgen Receptor: Complementary Deoxyribonucleic Acid Cloning, Sequence Analysis and Gene Expression in Prostate (original) (raw)
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1Laboratories for Reproductive Biology, and the Department of Pediatrics, Physiology, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Biology, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Biochemistry, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599
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1Laboratories for Reproductive Biology, and the Department of Pediatrics, Physiology, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Biology, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Biochemistry, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599
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1Laboratories for Reproductive Biology, and the Department of Pediatrics, Physiology, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Biology, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Biochemistry, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599
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1Laboratories for Reproductive Biology, and the Department of Pediatrics, Physiology, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Biology, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599; Department of Biochemistry, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27599
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Supported by Grants HD-16910, HD-04466, HD-21744, and P30-HD-18968 (recombinant DNA and histochemistry cores) from the National Institute of Child Health and Human Development Center for Population Research, and by NIH Grants NS-17479, T32-DK-07219, and by the Andrew W. Mellon Foundation.
Published:
01 December 1988
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Dennis B. Lubahn, David R. Joseph, Madhabananda Sar, Jiann-an Tan, Henry N. Higgs, Robert E. Larson, Frank S. French, Elizabeth M. Wilson, The Human Androgen Receptor: Complementary Deoxyribonucleic Acid Cloning, Sequence Analysis and Gene Expression in Prostate, Molecular Endocrinology, Volume 2, Issue 12, 1 December 1988, Pages 1265–1275, https://doi.org/10.1210/mend-2-12-1265
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Abstract
Androgenic hormones mediate their effects on male sex differentiation and development through a high affinity receptor protein. We report here cloning of the complete coding sequence of the human androgen receptor (hAR). By sequence homology hAR is a member of the nuclear receptor family, with closest sequence identity to the progesterone, mineralocorticoid, and glucocorticoid receptors. Regions of highest homology include the DNA-binding domain and a small region within the hydrophobic ligand-binding domain. Comparison of the deduced 919 amino acid sequence of hAR (98,999 mol wt) to the 902 amino acid sequence of rat AR (98,227 mol wt) reveals identical sequences in the DNA- and hormone-binding domains, with an overall homology of 85%. In human prostate, the major androgen receptor mRNA species is 10 kilobases while a less abundant mRNA is approximately 7 kilobases. Rabbit polyclonal antibodies were raised against a synthetic peptide from the N-terminal region of hAR. Immunocytochemical analysis of human prostate tissue demonstrated that AR is localized predominantly in nuclei of glandular epithelial cells.
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Author notes
Supported by Grants HD-16910, HD-04466, HD-21744, and P30-HD-18968 (recombinant DNA and histochemistry cores) from the National Institute of Child Health and Human Development Center for Population Research, and by NIH Grants NS-17479, T32-DK-07219, and by the Andrew W. Mellon Foundation.
Copyright © 1988 by The Endocrine Society
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