Cell death-induced regeneration in wing imaginal discs requires JNK signalling (original) (raw)
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RESEARCH ARTICLE| 01 April 2010
Supplemental Figure S3Fig. S3. Proliferation parameters after cell death induction. (A) M-phase cells after 10 hours of rpr induction analysed after the indicated times to compare anterior (A) and posterior (P) compartments, showing a high concentration of mitoses in the A compartment of induced discs. **, P<0.0005; *, _P_=0.002. The number of mitoses in the A compartment increases immediately after induction (0 hours) and continues at a high level 10 hours after induction. A slight decrease in the overall mitotic activity of control and experimental discs was detected at 2.5 hours, probably as a consequence of the temperature shift after induction (from 29°C to 17°C). (B,C) Clusters of mitotic cells in control (B) and _rpr_-induced (C) discs. (D,E) Control (D) and 10 hour _rpr-_induced (E) discs after BrdU dilution assay analysed 36 hours after BrdU pulse (blue, nuclei; grey, BrdU). The A/P boundary is indicated with a dashed line; anterior is to the left, posterior to the right. The incorporation of BrdU was analysed after chase intervals in the progeny of labelled cells. In this manner, incorporated BrdU in actively proliferating cells will be diminished by dilution in their progeny, resulting in a lower-intensity signal. In discs induced for 10 hours and after 36 hours of chase, we observed that the anterior region near the wound had little or no labelling, suggesting high proliferative activity. The P compartment showed higher-intensity labelling, suggesting lower proliferation in comparison to the A compartment. Scale bars: 15 µm.
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