Triterpenoids from Hippophae rhamnoides L. and Their Nitric Oxide Production-Inhibitory and DPPH Radical-Scavenging Activities (original) (raw)

Abstract

In our investigation on the chemical constituents of Hippophae rhamnoides L., the chloroform-soluble fraction of the 80% acetone extract of branch bark was observed to inhibit nitric oxide (NO) production in a lipopolysaccharide and recombinant mouse interferon-γ-activated murine macrophage-like cell line, RAW 264.7 cells. Two new triterpenoids, 2-_O-trans-p_-coumaroyl maslinic acid (1) and 2-_O_-caffeoyl maslinic acid (2), and three known triterpenoids, oleanolic acid (3), 3-_O-trans-p_-coumaroyl oleanolic acid (4), and 3-_O_-caffeoyl oleanolic acid (5), and 6-methoxy-2_H_-1-benzopyran (6) and β-sitosterol (7) were isolated from the branch bark extract. Their inhibitory activities on the production of NO in RAW 264.7 cells and radical-scavenging activities were examined.