The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions (original) (raw)
- Konstadinos Moissoglu3,12,
- Emeline Coleno1,2,4,
- Tianhong Wang3,
- Arthur Imbert5,6,7,
- Marie-Cécile Robert1,2,4,
- Marion Peter1,2,
- Racha Chouaib1,2,8,
- Thomas Walter5,6,7,
- Florian Mueller9,10,
- Kazem Zibara8,11,
- Edouard Bertrand1,2,4 and
- Stavroula Mili3
- 1Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS, 34293 Montpellier, France
- 2Equipe labélisée Ligue Nationale Contre le Cancer, University of Montpellier, CNRS, 34000 Montpellier, France
- 3Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland 20814, USA
- 4Institut de Génétique Humaine, University of Montpellier, CNRS, 34396 Montpellier, France
- 5MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, 77300 Fontainebleau, France
- 6Institut Curie, 75248 Paris Cedex, France
- 7INSERM, U900, 75248 Paris Cedex, France
- 8Biology Department, Faculty of Sciences-I, Lebanese University, Beirut, Lebanon
- 9Unité Imagerie et Modélisation, Institut Pasteur and CNRS UMR 3691, 75015 Paris, France
- 10C3BI, USR 3756 IP CNRS – Paris, France
- 11ER045, PRASE, DSST, Lebanese University, Beirut, Lebanon
- Corresponding authors: edouard.bertrand{at}igh.cnrs.fr, voula.mili{at}nih.gov
- ↵12 These authors contributed equally to this work.
Abstract
RNA localization and local translation are important for numerous cellular functions. In mammals, a class of mRNAs localize to cytoplasmic protrusions in an APC-dependent manner, with roles during cell migration. Here, we investigated this localization mechanism. We found that the KIF1C motor interacts with APC-dependent mRNAs and is required for their localization. Live cell imaging revealed rapid, active transport of single mRNAs over long distances that requires both microtubules and KIF1C. Two-color imaging directly revealed single mRNAs transported by single KIF1C motors, with the 3′UTR being sufficient to trigger KIF1C-dependent RNA transport and localization. Moreover, KIF1C remained associated with peripheral, multimeric RNA clusters and was required for their formation. These results reveal a widespread RNA transport pathway in mammalian cells, in which the KIF1C motor has a dual role in transporting RNAs and clustering them within cytoplasmic protrusions. Interestingly, KIF1C also transports its own mRNA, suggesting a possible feedback loop acting at the level of mRNA transport.
Footnotes
Article is online at http://www.rnajournal.org/cgi/doi/10.1261/rna.078576.120.
Freely available online through the RNA Open Access option.
Received December 7, 2020.
Accepted September 1, 2021.
© 2021 Pichon et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society
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Profile for Pichon, X.http://orcid.org/0000-0002-0755-2305
Profile for Moissoglu, K.http://orcid.org/0000-0001-7211-4320
Profile for Peter, M.http://orcid.org/0000-0002-5720-4798
Profile for Mueller, F.http://orcid.org/0000-0002-9622-4396
Profile for Bertrand, E.http://orcid.org/0000-0002-9642-7994
Profile for Mili, S.http://orcid.org/0000-0002-9161-8660