Effects of Silk Sericin on Incision Wound Healing in a Dorsal Skin Flap Wound Healing Rat Model (original) (raw)

01 April 2016 : Animal Research

Murat Ersel, _Yigit Uyanikgil_ABCDEFG, _Funda Karbek Akarca_BCD, _Enver Ozcete_BCD, _Yusuf Ali Altunci_BCD, _Fatih Karabey_ABCD, _Turker Cavucoglu_ABCD, _Ayfer Meral_CD, _Gurkan Yigitturk_BCD, _Emel Oyku Cetin_ADEFG

DOI: 10.12659/MSM.897981

Med Sci Monit 2016; 22:1064-1078

Abstract

BACKGROUND: The wound healing process is complex and still poorly understood. Sericin is a silk protein synthesized by silk worms (Bombyx mori). The objective of this study was to evaluate in vivo wound healing effects of a sericin-containing gel formulation in an incision wound model in rats.

MATERIAL AND METHODS: Twenty-eight Wistar-Albino rats were divided into 4 groups (n=7). No intervention or treatment was applied to the Intact control group. For other groups, a dorsal skin flap (9×3 cm) was drawn and pulled up with sharp dissection. The Sham operated group received no treatment. The Placebo group received placebo gel without sericin applied to the incision area once a day from day 0 to day 9. The Sericin Group 3 received 1% sericin gel applied to the incision area once a day from day 0 to day 9. Hematoxylin and eosin stain was applied for histological analysis and Mallory-Azan staining was applied for histoimmunochemical analysis of antibodies and iNOS (inducible nitric oxide synthase), and desmin was applied to paraffin sections of skin wound specimens. Parameters of oxidative stress were measured in the wound area.

RESULTS: Epidermal thickness and vascularization were increased, and hair root degeneration, edema, cellular infiltration, collagen discoloration, and necrosis were decreased in Sericin group in comparison to the Placebo group and the Sham operated group. Malonyldialdehyde (MDA) levels were decreased, but superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities were increased in the sericin group.

CONCLUSIONS: We found that sericin had significant positive effects on wound healing and antioxidant activity. Sericin-based formulations can improve healing of incision wounds.

Keywords: Biopsy, Catalase - metabolism, Chromatography, High Pressure Liquid, Collagen - metabolism, Edema - pathology, Epidermis - pathology, Glutathione Peroxidase - metabolism, Malondialdehyde - metabolism, Necrosis, Nitric Oxide Synthase Type II - metabolism, Placebos, Sericins - pharmacology, Skin - pathology, Superoxide Dismutase - metabolism, Surgical Flaps - pathology, Wound Healing - drug effects