Type II Antifreeze Protein from a Mid-latitude Freshwater Fish, Japanese Smelt (Hypomesus nipponensis) (original) (raw)

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Department of Biotechnology, Faculty of Engineering, Kansai University

3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan

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Department of Biotechnology, Faculty of Engineering, Kansai University

3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan

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Department of Biotechnology, Faculty of Engineering, Kansai University

3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan

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Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST)

2-17-2-1 Tsukisamu-Higashi, Toyohira, Sapporo 062-8517, Japan

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Department of Biotechnology, Faculty of Engineering, Kansai University

3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan

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Department of Biotechnology, Faculty of Engineering, Kansai University

3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan

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Accepted:

10 October 2002

Published:

01 January 2003

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Yasuhiro YAMASHITA, Rikako MIURA, Yukari TAKEMOTO, Sakae TSUDA, Hidehisa KAWAHARA, Hitoshi OBATA, Type II Antifreeze Protein from a Mid-latitude Freshwater Fish, Japanese Smelt (Hypomesus nipponensis), Bioscience, Biotechnology, and Biochemistry, Volume 67, Issue 3, 1 January 2003, Pages 461–466, https://doi.org/10.1271/bbb.67.461
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Abstract

A lot of reports of antifreeze protein (AFP) from fish have been published, but no report has mentioned of commercialized mid-latitude fresh water fish which producing AFP in its body fluid. We found that the AFP in the body fluid of Japanese smelt (Hypomesus nipponensis) from mid-latitude fresh water was purified and characterized. The N-terminal amino acid sequence of the Japanese smelt AFP was 75.0% identical to Type II AFP from herring. Results of EDTA treatment and ruthenium red staining suggested that the Japanese smelt AFP had at least one Ca2+-binding domain. Interestingly, the antifreeze activity of the Japanese smelt AFP did not completely disappear when Ca2+ ions were removed. The molecular mass of the Japanese smelt AFP was calculated to be 16,756.8 by the TOF-mass analysis. The Open reading flame of the gene coding for the Japanese smelt AFP was 444 bp long and was 85.0% identical with the entire herring AFP gene. The cDNA and amino acid sequence of the Japanese smelt AFP were the same length as those of herring AFP.

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© 2003 by Japan Society for Bioscience, Biotechnology, and Agrochemistry

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