Systemic Distribution of Salusin Expression in the Rat (original) (raw)

Hypertension Research volume 30, pages 1255–1262 (2007)Cite this article

Abstract

Salusin-α and salusin-β are multifunctional bioactive peptides with hypotensive and bradycardic effects. They were originally identified from full-length human cDNAs by bioinformatics analyses. Salusin peptides are expressed in human tissues at the mRNA level, but no information is available about their systemic distributions in any species. We examined the distributions of preprosalusin mRNA and the salusin peptides in a variety of normal rat organs. Whereas preprosalusin mRNA was expressed ubiquitously, immunoreactive salusin-β was detected most strongly in the hypothalamus and posterior pituitary, and less abundantly in the anterior pituitary and gastrointestinal, immune, and hematopoietic systems. Salusin-β−positive cells appeared to be of either hematopoietic or endocrine origin, and many hematopoietic cells were also stained with anti-CD68, which specifically recognizes macrophages. Salusin-α−like immunoreactivity was not detected in any of the rat tissues. These results indicate that rat salusin is immunologically similar to human salusin-β and widely expressed, especially in the immune, gastrointestinal, and central nervous systems and mainly in endocrine- and hematopoietic-derived cells.

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  1. Shichiri M, Ishimaru S, Ota T, Nishikawa T, Isogai T, Hirata Y : Salusins: newly identified bioactive peptides with hemodynamic and mitogenic activities. Nat Med 2003; 9: 1166–1172.
    Article CAS Google Scholar
  2. Izumiyama H, Tanaka H, Egi K, Sunamori M, Hirata Y, Shichiri M : Synthetic salusins as cardiac depressors in rat. Hypertension 2005; 45: 419–425.
    Article CAS Google Scholar
  3. Yu F, Zhao J, Yang J, et al: Salusins promote cardiomyocyte growth but does not affect cardiac function in rats. Regul Pept 2004; 122: 191–197.
    Article CAS Google Scholar
  4. Xiao-Hong Y, Li L, Yan-Xia P, et al: Salusins protect neonatal rat cardiomyocytes from serum deprivation-induced cell death through upregulation of GRP78. J Cardiovasc Pharmacol 2006; 48: 41–46.
    Article Google Scholar
  5. Wang Z, Takahashi T, Saito Y, et al: Salusin beta is a surrogate ligand of the mas-like G protein–coupled receptor MrgA1. Eur J Pharmacol 2006; 539: 145–150.
    Article CAS Google Scholar
  6. Takenoya F, Hori T, Kageyama H, et al: Coexistence of salusin and vasopressin in the rat hypothalamo-hypophyseal system. Neurosci Lett 2005; 385: 110–113.
    Article CAS Google Scholar
  7. Yoshimoto T, Gochou N, Fukai N, Sugiyama T, Shichiri M, Hirata Y : Adrenomedullin inhibits angiotensin II–induced oxidative stress and gene expression in rat endothelial cells. Hypertens Res 2005; 28: 165–172.
    Article CAS Google Scholar
  8. Damoiseaux JG, Dopp EA, Calame W, Chao D, MacPherson GG, Dijkstra CD : Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1. Immunology 1994; 83: 140–147.
    CAS PubMed PubMed Central Google Scholar
  9. Dijkstra CD, Dopp EA, Joling P, Kraal G : The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology 1985; 54: 589–599.
    CAS PubMed PubMed Central Google Scholar
  10. Sato K, Koyama T, Tateno T, Hirata Y, Shichiri M : Presence of immunoreactive salusin-alpha in human serum and urine. Peptides 2006; 27: 2561–2566.
    Article CAS Google Scholar

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Authors and Affiliations

  1. Department of Clinical and Molecular Endocrinology, Tokyo Medical and Dental University, Tokyo, Japan
    Noriko Suzuki, Masayoshi Shichiri, Maya Sakurada, Yuki Hirono, Takanobu Yoshimoto & Yukio Hirata
  2. Department of Pathology, Tokyo Medical and Dental University, Tokyo, Japan
    Takumi Akashi
  3. Graduate School of Health Sciences, Tokyo Medical and Dental University, Tokyo, Japan
    Kengo Sato & Takatoshi Koyama

Authors

  1. Noriko Suzuki
  2. Masayoshi Shichiri
  3. Takumi Akashi
  4. Kengo Sato
  5. Maya Sakurada
  6. Yuki Hirono
  7. Takanobu Yoshimoto
  8. Takatoshi Koyama
  9. Yukio Hirata

Corresponding author

Correspondence toMasayoshi Shichiri.

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Suzuki, N., Shichiri, M., Akashi, T. et al. Systemic Distribution of Salusin Expression in the Rat.Hypertens Res 30, 1255–1262 (2007). https://doi.org/10.1291/hypres.30.1255

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