Method of obtaining optical sectioning by using structured light in a conventional microscope (original) (raw)

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Author Affiliations

M. A. A. Neil, R. Juškaitis, and T. Wilson

Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK

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Abstract

We describe a simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object. Images taken at three spatial positions of the grid are processed in real time to produce optically sectioned images that are substantially similar to those obtained with confocal microscopes.

© 1997 Optical Society of America

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