Determination of Veterinary Pharmaceuticals in Production Wastewater by HPTLC-Videodensitometry (original) (raw)

Abstract

An SPE-HPTLC method for simultaneous identification and quantification of seven pharmaceuticals in production wastewater was optimized and validated. The studied compounds were enrofloxacine, oxytetracycline, trimethoprim, sulfamethazine, sulfadiazine, sulfaguanidine and penicillin G/procaine. The method involves solid-phase extraction on hydrophilic-lipophilic balance cartridges with methanol and HPTLC analysis of extracts on CN modified chromatographic plates followed by videodensitometry at 254 and 366 nm. Optimization of chromatographic separation was performed by systematic variation of the mobile phase composition using genetic algorithm approach and the optimum mobile phase composition for TLC separation was 0.05 M H2C2O4:methanol = 0.81:0.19 (v/v). Linearity of the method was demonstrated in the ranges from 1.5 to 15.0 µg L−1 for enrofloxacine, 100–500 µg L−1 for oxytetracycline, 150–600 µg L−1 for trimethoprim, 300–1100 µg L−1 for sulfaguanidine and 100–400 µg L−1 for sulfamethazine, sulfadiazine and penicillin G/procaine with coefficients of determination higher than 0.991. Mean recoveries ranged from 74.6 to 117.1% and 55.1 to 108.0% for wellspring water and production wastewater, respectively. Only sulfaguanidine showed lower results. The described method has been applied to the determination of pharmaceuticals in wastewater samples from pharmaceutical industry.

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References

  1. McDonald LR, Roberson EL (1991) Veterinary pharmacology, therapeutics, In: McDonald LE (ed) Iowa State University Press, Ames-Iowa, pp 897–902
  2. Jones OAH, Voulvoulis N, Lester JN (2002) Wat Res 36:5013–5022
    Article CAS Google Scholar
  3. Heberer T (2002) Tox Letters 131:5–17
    Article CAS Google Scholar
  4. Polestra K, Bakker WW, Klok PA, Kamps JA, Hardonk MJ, MijerDK (1997) Am J Pathol 151(4):1163–1169
    Google Scholar
  5. Carballa M, Omil F, Lema JM, Llompart M, Garcia-Jares C, Rodriguez I, Gomez M, Ternes T (2004) Water Res 38:2918–2926
    Article PubMed CAS Google Scholar
  6. Daughton D, Ternes TA (1999) Environ Health Perspect 107(6):907–938
    PubMed CAS Google Scholar
  7. Niessen WMA (1998) J Chromatogr A 812:53–75
    Article PubMed CAS Google Scholar
  8. Castiglioni S, Bagnati R, Calamari D, Fanelli R, Zuccato E (2005) J Chromatogr 1092:206–215
    Article CAS Google Scholar
  9. Reeves VB (1999) J Chromatogr B 723:127–137
    CAS Google Scholar
  10. Takeda N, Akiyama Y (1992) J Chromatogr A 607:31–35
    Article CAS Google Scholar
  11. Vinas P, Erroz CL, Campillo N, Cordoba HM (1996) J Chromatogr A, 726:125–131–140
    Google Scholar
  12. Reemtsma T (2001) Trends Anal Chem 20:533–542
    Article CAS Google Scholar
  13. Hernando MD, Petrović M, Fernandez-Alba AR, Barcelo D (2004) J Chromatogr A 1046:133–140
    Article PubMed CAS Google Scholar
  14. Cimpoui C (2003) Handbook of thin-layer chromatography, In: Sherma J, Fried B (eds) Marcel Dekker, New York, pp 81–98
  15. De Spiegeleer BMJ, De Moerloose PHM (1987) Anal. Chem. 59:62–64
    Article Google Scholar
  16. Babić S, Horvat AJM, Kaštelan-Macan M (2005) JPC-J. Planar Chromatogr.-Modern TLC 18:112–117
    Google Scholar
  17. Nagaraja P, Yathirajan HS, Raju CR, Vasantha RA, Nagendra P, Hemantha Kumar MS (2003) Il Farmaco 58:1295–1300
    Article PubMed CAS Google Scholar

Download references

Acknowledgments

This work has been supported by: EU project EMCO (INCO CT 2004-509188) -Reduction of environmental risks, posed by Emerging Contaminants, through advanced treatment of municipal and industrial wastes and Croatian Ministry of Education, Science and Sport (Project No. 0125055).

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Authors and Affiliations

  1. Laboratory of Analytical Chemistry, Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 20, 10000, Zagreb, Croatia
    S. Babić, D. Mutavdžić, D. Ašperger, A. J. M. Horvat & M. Kaštelan-Macan

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  1. S. Babić
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  2. D. Mutavdžić
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  3. D. Ašperger
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  4. A. J. M. Horvat
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  5. M. Kaštelan-Macan
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Correspondence toS. Babić.

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Babić, S., Mutavdžić, D., Ašperger, D. et al. Determination of Veterinary Pharmaceuticals in Production Wastewater by HPTLC-Videodensitometry.Chroma 65, 105–110 (2007). https://doi.org/10.1365/s10337-006-0109-2

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