Aqueous Two-Phase Systems (original) (raw)
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Editors:
- Rajni Hatti-Kaul
- Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden
Includes supplementary material: sn.pub/extras
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A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
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Table of contents (38 protocols)
Preparation and Characterization of Aqueous Two-Phase Systems
Partitioning Techniques
Partitioning of Soluble Molecules and Particulates
Editors and Affiliations
Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden
Rajni Hatti-Kaul
Bibliographic Information
- Book Title: Aqueous Two-Phase Systems
- Book Subtitle: Methods and Protocols
- Editors: Rajni Hatti-Kaul
- Series Title: Methods in Biotechnology
- DOI: https://doi.org/10.1385/1592590284
- Publisher: Humana Totowa, NJ
- eBook Packages: Springer Protocols
- Copyright Information: Humana Press 2000
- Hardcover ISBN: 978-0-89603-541-6Published: 21 February 2000
- Softcover ISBN: 978-1-61737-067-0Published: 19 November 2010
- eBook ISBN: 978-1-59259-028-5Published: 05 February 2008
- Series ISSN: 1940-6061
- Series E-ISSN: 1940-607X
- Edition Number: 1
- Number of Pages: XIV, 454
- Topics: Biotechnology