Preparation of HeLa Nuclear Extracts (original) (raw)

References

  1. Dignam, J. D., Lebovitz, R. M., and Roeder, R. G. (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammahan nuclei. Nucleic Acids Res. 11, 1475–1489.
    Article PubMed CAS Google Scholar
  2. Kelleher, R. J., III, Flanagan, P. M., and Kornberg, R. D (1990) A novel mediator between activator proteins and the RNA polymerase II transcription apparatus. Cell 61, 1209–1215.
    Article PubMed CAS Google Scholar
  3. Biggin, M. D. and Tjian, R. (1988) Transcription factors that activate the Ultrabithorax promoter m developmentally staged extracts. Cell 53, 699–711.
    Article PubMed CAS Google Scholar
  4. Peterson, M. G., Inostroza, J., Maxon, M. E., Flores, O., Admon, A., Reinberg, D., and Tjian, R. (1991) Primary structure and functional properties of recombinant human transcription factor TFIIE. Nature 354, 369–373.
    Article PubMed CAS Google Scholar
  5. Peterson, M. G., Tanese, N., Pugh, B. F., and Tjian, R. (1990) Functional domains and upstream activation properties of cloned human TATA-binding protein. Science 248, 1625–1630.
    Article PubMed CAS Google Scholar
  6. Kao, C. C., Lieberman, P. M., Schmidt, M. C., Zhou, Q., Pei, R., and Berk, A. J. (1990) Cloning of a transcriptionally active human TATA-binding factor. Science 248, 1646–1650.
    Article PubMed CAS Google Scholar
  7. Hoffmann, A., Sinn, E., Yamamoto, T., Wang, J., Roy, A., Horikoshi, M., and Roeder, R. G. (1990) Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID). Nature 346, 387–390.
    Article CAS Google Scholar
  8. Ha, I., Lane, W. S., and Reinberg, D. (1991) Cloning and structure of a human gene encoding the general transcription initiation factor IIB. Nature 352, 689–694.
    Article PubMed CAS Google Scholar
  9. Sopta, M., Burton, Z. F., and Greenblatt, J. (1989) Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II. Nature 341, 410–414.
    Article PubMed CAS Google Scholar
  10. Finkelstein, A., Kostrub, C. F., Li, J., Chavez, D. P., Wang, B. Q., Fang, S. M., Greenblatt, J., and Burton, Z. F. (1992) A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II. Nature 355, 464–467.
    Article PubMed CAS Google Scholar
  11. Nakajima, N., Horikoshi, M., and Roeder, R. G. (1988) Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID. Mol. Cell. Biol. 8, 4028–4040.
    PubMed CAS Google Scholar
  12. Jackson, S. P. and Tjian, R. (1988) _O_-Glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation, Cell 55, 125–133.
    Article PubMed CAS Google Scholar
  13. Jackson, S. P. and Tjian, R. (1989) Purification and analysis of RNA polymerase II transcription factors by using wheat-germ agglutinin affinity chromatography. Proc. Natl. Acad. Sci. USA 86, 1781–1785.
    Article PubMed CAS Google Scholar
  14. Carey, M., Leatherwood, J., and Ptashne, M. (1990) A potent GAL4 derivative activates transcription at a distance in vitro. Science 247, 710–712.
    Article PubMed CAS Google Scholar
  15. Pugh, B. F. and Tjian, B. F. (1991) Transcription from a TATA-less promoter requires a multisubunit TFIID complex. Genes Devel. 5, 1935–1945.
    Article PubMed CAS Google Scholar

Download references