Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations (original) (raw)

References

  1. Wu, R. S, Panusz, H. T. Hatch, C L. and Bonner, W. M. (1986) Histones and their modifications CRC Crtt Rev. Biochem. 20, 201–263
    Article CAS Google Scholar
  2. Jeppesen, P., Mitchell, A, Turner, B, and Perry, P. (1992) Antibodies to defined histone epitopes reveal variations in chromatin conformation and under-acetylation of centric heterochromatin in human metaphase chromosomes. Chromosoma 101, 322–332
    Article PubMed CAS Google Scholar
  3. Jeppesen, P. and Bower, D. J. (1986) Towards understanding the structure of eukaryotic chromosomes, in Cytogenetics: Basic and Applied Aspects (Obe, G. and Basler, A., eds.) Spnnger-Verlag, Berlin, pp 1–29
    Google Scholar
  4. Cooke, C. A., Bernat, R. L., and Earnshaw, W. C. (1990) CENP-B: a major human centromere protein located beneath the kinetochore J. Cell Biol. 110, 1475–1488.
    Article PubMed CAS Google Scholar
  5. Compton, D A, Yen, T J, and Cleveland, D. W (1991) Identification of novel centromere/kinetochore-associated proteins using monoclonal antibodies generated against human mitotic chromosome scaffolds J. Cell Biol. 112, 1083–1097
    Article PubMed CAS Google Scholar
  6. Earnshaw, W. C, Halligan, B, Cooke, C. A, Heck, M M S., and Lin, L. F. (1985) Topoisomerase II is a structural component of mitotic chromosome scaffolds. J. Cell Biol. 100, 1707–1715
    Google Scholar
  7. Tan, E. M. (1982) Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine Adv Immunol 33, 167–240
    Article PubMed CAS Google Scholar
  8. Moroi, Y., Peebles, C, Fritzler, M J., Steigerwald, J, and Tan, E. M. (1980) Autoantibody to centromere (kinetochore) in scleroderma sera Proc. Natl Acad.Sci USA 77, 1627–1631.
    Article PubMed CAS Google Scholar
  9. Earnshaw, W. C, Sullivan, K. F. Machlin, P. S., Cook, C. A., Kaiser, D. A., Pollard, T D, Rothfield, N. F, and Cleveland, D. W (1987) Molecular clon ing of cDNA for CENP-B, the major human centromere autoantigen. J. Cell Biol 104, 817–829.
    Article PubMed CAS Google Scholar
  10. Earnshaw, W. C. and Rothfield, N. (1985) Identification of a family of human centromere proteins using autoimmune sera from patients with scleroderma. Chromosoma 91, 313–321
    Article PubMed CAS Google Scholar
  11. Palmer, D. K. O’Day, K., Wener, M. H, Andrews, B. S, and Margolis, R L (1987) A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones J Cell Biol. 104, 805–815.
    Article PubMed CAS Google Scholar
  12. Gooderham, K. and Jeppesen, P. (1983) Chinese hamster metaphase chromosomes isolated under physiological conditions: a partial characterization of associated non-histone proteins and protein cores. Exp. Cell Res. 144, 1–14.
    Article PubMed CAS Google Scholar
  13. Jeppesen, P and Nicol, L (1986) Non-kinetochore directed autoantibodies in scleroderma/CREST-identification of an activity recognizing a metaphase chromosome core nonhistone protein Mol Biol Med. 3, 369–384.
    PubMed CAS Google Scholar
  14. Johnson, G D and Nogueira Araujo, G. M. de C. (1981) A simple method of reducing the fading of immunofluorescence during microscopy. J. Immunol Meth 43, 349–350
    Article CAS Google Scholar
  15. Giloh, H. and Sedat, J. W. (1982) Fluorescence microscopy: reduced photo-bleaching of rhodamine and fluorescein protein conjugates by n-propylgallate. Science 217, 1252–1255.
    Article PubMed CAS Google Scholar
  16. Haaf, T., Dominguez-Steghch, M., and Schmid, M. (1990) Immuno-cytogenetics: IV. Human autoantibodies to heterochromatin-associated proteins Cytogenet Cell Genet 53, 40–51
    Article PubMed CAS Google Scholar
  17. Whitfield, G F, Fellows, G., and Turner, B. M. (1986) Characterization of monoclonal antibodies to histone 2B Localization of epitopes and analysis of binding to chromatin. Eur. J. Bwchem 157, 513–521
    CAS Google Scholar
  18. Donlon, T A and Magenis, R. E. (1983) Methyl green is a substitute for distamycin A in the formation of distamycin A/DA PI C-bands. Hum Genet 65, 144–146
    Article PubMed CAS Google Scholar
  19. Turner, B. M and Fellows, G (1989) Specific antibodies reveal ordered and cell-cycle-related use of histone-H4 acetylation sites in mammalian cells. Eur. J. Biochem. 179, 131–139.
    Article PubMed CAS Google Scholar
  20. Mitchell, A., Jeppesen, P., Hanratty, D., and Gosden, J. (1992) The organization of repetitive DNA sequences on human chromosomes with respect to the kinetochore analyzed using a combination of oligonucleotide primers and CREST anti-centromere serum. Chromosoma 101, 333–341.
    Article PubMed CAS Google Scholar

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