Preparation of Bacterial Genomic DNA (original) (raw)

Abstract

The isolation of genomic DNA from a microorganism generally comprises three stages: cultivation of the cells, disruption to release cell contents, and chemical purification of the DNA. Two widely used methods for the preparation of bacterial DNA are those described by Marmur (1) and Kirby (2), but procedures are frequently modified to suit the particular organisms under study.

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References

  1. Marmur, J. (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3, 208–218.
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  2. Kirby, K. S. (1964) Isolation and fractionation of nucleic acids. Progr. Nucl. Acids Res. 3, 1.
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Author information

Authors and Affiliations

  1. Analytical Molecular Biology, Laboratory of the Government Chemist, Middlesex, UK
    Jane Bickley
  2. National Collection of Type Cultures, Central Public Health Laboratory, London, UK
    Robert J. Owen

Authors

  1. Jane Bickley
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  2. Robert J. Owen
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Editor information

Editors and Affiliations

  1. Dalgety plc, Cambridge, UK
    Jenny Howard
  2. Department of Medicine, University of Cambridge, UK
    David M. Whitcombe

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© 1995 Humana Press Inc.

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Bickley, J., Owen, R.J. (1995). Preparation of Bacterial Genomic DNA. In: Howard, J., Whitcombe, D.M. (eds) Diagnostic Bacteriology Protocols. Methods in Molecular Biology™, vol 46. Humana Press. https://doi.org/10.1385/0-89603-297-3:141

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