Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs (original) (raw)
References
Frohman, M. A., Dush, M. K., and Martin, G. R. (1988) Rapid production of full-length cDNAs from rare transcripts by amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA85, 8998–9002. ArticlePubMedCAS Google Scholar
Loh, E. L., Elliott, J. F., Cwirla, S., Lanier, L. L., and Davis, M. M. (1989) Polymerase chain reaction with single sided specificity: analysis of T cell receptor delta chain. Science243, 217–220. ArticlePubMedCAS Google Scholar
Ohara, O., Dorit, R. I., and Gilbert, W. (1989) One-sided PCR: The amplification of cDNA. Proc. Natl. Acad. Sci. USA86, 5673–5677. ArticlePubMedCAS Google Scholar
Frohman, M. A. (1989) Creating full-length cDNAs from small fragments of genes, amplification of rare transcripts using a single gene-specific oligonucleotide primer, in PCR Protocols and Applications: A Laboratory Manual (Innis, M., Gelfand, D., Sninsky, J., and White, T., eds.), Academic, San Diego, CA, pp. 28–38. Google Scholar
Frohman, M A. and Martin, G. R. (1989) Rapid amplification of cDNA ends using nested primers. Techniques1, 165–173. Google Scholar
Dumas, J. B., Edwards, M., Delort, J., and Mallet, J. (1991) Oligodeoxy-ribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5′ ends of mRNAs and for constructing cDNA libraries by In vitro amplification. Nucleic Acids Res.19, 5227–5233. Article Google Scholar
Fritz, J. D., Greaser, M. L., and Wolff, J. A. (1991) A novel 3′ extension technique using random primers in RNA-PCR. Nucleic Acids Res.119, 3747. Article Google Scholar
Borson, N. D., Salo, W. L., and Drewes, L. R. (1992) A lock-docking oligo(dT) primer for 5′ and 3′ RACE PCR. PCR Methods Appl.2, 144–148. PubMedCAS Google Scholar
Jain, R., Gomer, R.H., and Murtagh, J. J., Jr. (1992) Increasing specificity from the PCR-RACE technique. BioTechniques12, 58–59. PubMedCAS Google Scholar
Rashtchian, A., Buchman, G. W., Schuster, D. M., and Berninger, M. S. (1992) Uracil DNA glycosylase-mediated cloning of PCR-amplified DNA: application to genomic and cDNA cloning. Anal. Biochem.206, 91–97. ArticlePubMedCAS Google Scholar
Schuster, D. M., Buchman, G. W., and Rastchian, A. (1992) A simple and efficient method for amplification of cDNA ends using 5′ RACE. Focus14, 46–52. Google Scholar
Bertling, W. M., Beier, F., and Reichenberger, E. (1993) Determination of 5′ ends of specific mRNAs by DNA ligase-dependent amplification. PCR Methods Appl.3, 95–99. PubMedCAS Google Scholar
Frohman, M. A. (1993) Rapid amplification of cDNA for generation of full-length cDNA ends: thermal RACE. Methods Enzymol.218, 340–356. ArticlePubMedCAS Google Scholar
Monstein, H. J., Thorup, J. U., Folkesson, R., Johnsen, A. H., and Rehfeld, J. F. (1993) cDNA deduced procionin-structure and expression in protochordates resemble that of procholecystokinin in mammals. FEBS Lett.331, 60–64. ArticlePubMedCAS Google Scholar
Templeton, N. S., Urcelay, E., and Safer, B. (1993) Reducing artifact and increasing the yield of specific DNA target fragments during PCR-RACE or anchor PCR. BioTechniques15, 48–50. PubMedCAS Google Scholar
Frohman, M. A. (1994) Cloning PCR products: strategies and tactics, in Methods in Molecular Biology: PCR: The Polymerase Cham Reaction (Mullis, K. B., Ferre, F., and Gibbs, R. A., eds.), Birkhauser, Boston, MA, pp. 14–37. Google Scholar
Datson, N. A., Duyk, G.M., Van Ommen, J. B., and Den Dunnen, J. T. (1994) Specific isolation of 3′-terminal exons of human genes by exon trapping. Nucleic. Acids. Res.22, 4148–4153. ArticlePubMedCAS Google Scholar
Ruberti, F., Cattaneo, A., and Bradbury, A. (1994) The use of the RACE method to clone hybridoma cDNA when V region primers fail. J. Immunol. Methods173, 33–39. ArticlePubMedCAS Google Scholar
Tessier, D. C., Brousseau, R., and Vernet, T. (1986) Ligation of single-stranded oligodeoxyribonucleotides by T4 RNA ligase. Anal. Biochem.158, 171–178. ArticlePubMedCAS Google Scholar
Mandl, C. W., Heinz, F. X., Puchhammer-Stockl, E., and Kunz, C. (1991) Sequencing the termini of capped viral RNA by 5′-3′ ligation and PCR. BioTechniques10, 484–486. PubMedCAS Google Scholar
Volloch, V., Schweizer, B., Zhang, X., and Rits, S. (1991) Identification of negative-strand complements to cytochrome oxidase subunit III RNA in Trypanosoma brucei. Biochemistry88, 10,671–10,675. CAS Google Scholar
Brock, K. V., Deng, R., and Riblet, S. M. (1992) Nucleotide sequencing of 5′ and 3′ termini of bovine viral diarrhea virus by RNA ligation and PCR. Virol. Methods38, 39–46. ArticleCAS Google Scholar
Bertrand, E., Fromont-Racine, M., Pictet, R., and Grange, T. (1993) Visualization of the interaction of a regulatory protein with RNA in vivo. Proc. Natl. Acad. Sci. USA90, 3496–3500. ArticlePubMedCAS Google Scholar
Fromont-Racine, M., Bertrand, E., Pictet, R., and Grange, T. (1993) A highly sensitive method for mapping the 5′ termini of mRNAs. Nucleic Acids Res.21, 1683,1684. ArticlePubMedCAS Google Scholar
Liu, X. and Gorovsky, M. A. (1993) Mapping the 5′ and 3′ ends of tetrahymenathermophila mRNAs using RNA Ligase mediated amplification of cDNA ends (RLM-RACE). Nucleic Acids Res.21, 4954–4660. ArticlePubMedCAS Google Scholar
Sallie, R. (1993) Characterization of the extreme 5′ ends of RNA molecules by RNA ligation-PCR. PCR Methods Applic.3, 54–56. CAS Google Scholar
Skinner, T. L., Kerns, R. T., and Bender, P. K. (1994) Three different calmodulin-encoding cDNAs isolated by a modified 5′-RACE using degenerate oligo-deoxyribonucleotides. Gene151, 247–251. ArticlePubMedCAS Google Scholar
Frohman, M. A., Dickinson, M. E., Hogan, B. L. M., and Martin, G. R. (1993) Localization of two new and related homeobox-containing genes to chromosomes 1 and 5, near the phenotypically similar mutant loci dominant hemimelia (Dh) and hemimelic extra-toes (Hx). Mouse Genome91, 323–325. Google Scholar
Crowe, J. S., Cooper, H. J., Smith, M. A., Sims, M. J., Parker, D., and Gewert, D. (1991) Improved cloning efficiency of polymerase cham reaction (PCR) products after proteinase K digestion. Nucleic Acids Res.19, 184. ArticlePubMedCAS Google Scholar
Coleclough, C. (1987) Use of primer-restriction end adapters in cDNA cloning Methods Enzymol.154, 64–83. ArticlePubMedCAS Google Scholar
Don, R. H, Cox, P. T, Wainwright, B. J., Baker, K., and Mattick, J. S. (1991) Touchdown PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res.19, 4008. ArticlePubMedCAS Google Scholar
Mead, D. A., Pey, N. K., Herrnstadt, C., Marcil, R. A., and Smith, L. A. (1991) A universal method for direct cloning of PCR amplified nucleic acid. Biotechnology9, 657–663. ArticlePubMedCAS Google Scholar
Marchuk, D., Drumm, M., Saulino, A., and Collins, F. S. (1991) Construction of T-vector, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res.19, 1154. ArticlePubMedCAS Google Scholar
Kovalic, D., Kwak, J. H., and Weisblum, B. (1991) General method for direct cloning of DNA fragments generated by the polymerase chain reaction. Nucleic Acids Res.19, 4650. Article Google Scholar
Holton, T. A. and Graham, M. W. (1991) A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors. Nucleic Acids Res.19, 1156. ArticlePubMedCAS Google Scholar
Stoker, A. W. (1990) Cloning of PCR products after defined cohesive termini are created with T4 DNA polymerase. Nucleic Acids Res.18, 4290. ArticlePubMedCAS Google Scholar
Iwahana, H., Mizusawa, N., Ii, S., Yoshimoto, K., and Itakura, M. (1994) An end-trimming method to amplify adjacent cDNA fragments by PCR. BioTechniques16, 94–98. PubMedCAS Google Scholar
Thweatt, R., Goldstein, S., and Reis, R. J. S. (1990) A universal primer mixture for sequence determination at the 3′ ends of cDNAs. Anal. Biochem.190, 314 ArticlePubMedCAS Google Scholar
Eckert, K. A and Kunkel, T. A. (1990) High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Nucleic Acids Res.18, 3739–3745. ArticlePubMedCAS Google Scholar
Sambrook, J., Fritsch, E. F., and Mamatis, T. (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. Google Scholar
Sarker, G., Kapelner, S., and Sommer, S. S. (1990) Formamide can dramatically improve the specificity of PCR. Nucleic Acids Res.18, 7465. Article Google Scholar