Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells (original) (raw)

References

1. Moll, H. (ed.) (1995) The immune functions of epidermal Langerhans cells. R.G.Landes Company / Springer-Verlag. Austin.
   Google Scholar
2. Schuler, G. (ed.) (1991) Epidermal Langerhans cells. CRC Press Inc., Boca Raton, FL.
   Google Scholar
3. Steinman, R.M. (1991) The dendritic cell system and its role in immunogenicity. Annu. Rev. Immunol., 9, 271–296.
   Article PubMed CAS Google Scholar
4. Maurer, D. and Stingl, G. (1998) Dendritic cells in the context of skin immunology. In Dendritic cells: biology and clinical applications, Lotze, M. T. and Thomson, A. W. (eds.) Academic Press, London.
   Google Scholar
5. Banchereau, J. and Steinman, R.M. (1998) Dendritic cells and the control of immunity. Nature, 392, 245–252.
   Article PubMed CAS Google Scholar
6. Langerhans, P. (1868) Über die Nerven der menschlichen Haut. Virchows Arch.[A], 44, 325–337.
   Google Scholar
7. Romani, N., Witmer-Pack, M., Crowley, M., et al. (1991) Langerhans cells as immature dendritic cells. In Epidermal Langerhans cells, Schuler, G. (ed.) CRC Press Inc., Boca Raton, FL, pp. 191–216.
   Google Scholar
8. Schuler, G. and Steinman, R.M. (1985) Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro. J. Exp. Med., 161,526–546.
   Article PubMed CAS Google Scholar
9. Romani, N., Koide, S., Crowley, M., et al. (1989) Presentation of exogenous protein antigens by dendritic cells to T cell clones: intact protein is presented best by immature epidermal Langerhans cells. J.Exp. Med., 169, 1169–1178.
   Article PubMed CAS Google Scholar
10. Lappin, M.B., Kimber, I., and Norval, M. (1996) The role of dendritic cells in cutaneous immunity. Arch. Dermatol. Res. 288, 109–121.
    Article PubMed CAS Google Scholar
11. Knop, J. and Enk, A.H. (1995) Cellular and molecular mechanisms in the induction phase of contact sensitivity. Int. Arch. Allergy Immunol., 107, 231–232.
    Article PubMed CAS Google Scholar
12. Caux, C., Massacrier, C., Vanbervliet, B., et al. (1997) CD34+hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to granulocyte-macrophage colony-stimulating factor plus tumor necrosis factor α.2. Functional analysis. Blood, 90,1458–1470.
    PubMed CAS Google Scholar
13. Inaba, K., Inaba, M., et al. (1992) Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J. Exp. Med. 176,1693–1702.
    Article PubMed CAS Google Scholar
14. Schuler, G. and Koch, F. (1991) Enrichment of epidermal Langerhans cells. In Epidermal Langerhans cells, Schuler, G. (ed.) CRC Press Inc., Boca Raton, FL., pp. 139–157.
    Google Scholar
15. Koch, F., Kämpgen, E., Schuler, G. and Romani, N. (1992) Effective enrichment of murine epidermal Langerhans cells by a modified-“mismatched”-panning technique. J. Invest. Dermatol. 99, 803–807.
    Article PubMed CAS Google Scholar
16. Romani, N., Bhardwaj, N., Pope, M., et al. (1997) Dendritic cells. In Weir’s Handbook of Experimental Immunology, Herzenberg, L.A., Weir, D.M., Herzenberg, L. and Blackwell, C. (eds.) Blackwell Science, Oxford, pp. 156.1–156.14.
    Google Scholar
17. Schuler, G., Lutz, M. B., Bender, A., Thurner, B., Röder, C. and Romani, N. (1998) A guide to the isolation and propagation of dendritic cells. In Dendritic cells: biology and clinical applications, Lotze, M. T. and Thomson, A. W. (eds.) Academic Press, London.
    Google Scholar
18. Inaba, K., Swiggard, W. J., Steinman, R. M., Romani, N. and Schuler, G. (1998) Isolation of dendritic cells. In current protocols in immunology, Coligan, J. E., Kruisbeek, A. M., Margulies, D. H., Shevach, E. M. and Strober, W. (eds.) Wiley, New York, pp. 3.7.1–3.7.15.
    Google Scholar
19. Tschachler, E., Schuler, G., Hutterer, J., Leibl, H., Wolff, K., and Stingl, G. (1983) Expression of Thy-1 antigen by murine epidermal cells. J. Invest. Dermatol., 81, 282–285.
    Article PubMed CAS Google Scholar
20. Bergstresser, P. R., Tigelaar, R. E., Dees, J. H., and Streilein, J. W. (1983) Thy-1 antigen bearing dendritic cells populate murine epidermis. J. Invest. Dermatol., 81, 286–288.
    Article PubMed CAS Google Scholar
21. Wood, G. S., Kosek, J., Butcher, E. C. and Morhenn, V. B. (1985) Enrichment of murine and human Langerhans cells with solid phase immunoabsorption using pan-leukocyte monoclonal antibodies. J. Invest. Dermatol., 84, 37–40.
    Article PubMed CAS Google Scholar
22. Witmer-Pack, M. D., Olivier, W., Valinsky, J., Schuler, G., and Steinman, R. M. (1987) Granulocyte/macrophage colony-stimulating factor is essential for the viability and function of cultured murine epidermal Langerhans cells. J. Exp. Med., 166,1484–1498.
    Article PubMed CAS Google Scholar
23. Heufler, C., Koch, F., and Schuler, G. (1988) Granulocyte-macrophagecolony-stimulating factor and interleukin-1 mediate the maturation of murine epidermal Langerhans cells into potent immunostimulatory dendritic cells. J. Exp. Med., 167,700–705.
    Article PubMed CAS Google Scholar
24. Koch, F., Heufler, C., Kämpgen, E., Schneeweiss, D., Böck, G., and Schuler, G. (1990) Tumor necrosis factor alpha maintains the viability of murine epidermal Langerhans cells in culture but in contrast to granulocyte/macrophage colony-stimulating factor without inducing their functional maturation. J. Exp. Med. 171,159–171.
    Article PubMed CAS Google Scholar
25. Koch, F., Kämpgen, E., and Trockenbacher, B. (1995) TNFa interrupts antigenpresenting function of Langerhans cells by two mechanisms: loss of immunogenic pep tides and impairment of antigen-independent T cell clustering. Adv. Exp. Med. Biol., 378,207–209.
    PubMed CAS Google Scholar
26. Kraal, G., Breel, M., Janse, M. and Bruin, G. (1986) Langerhans’ cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J. Exp. Med. 163, 981–997.
    Article PubMed CAS Google Scholar
27. Inaba, K., Witmer-Pack, M., Inaba, M., et al. (1994) The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J. Exp. Med., 180, 1849–1860.
    Article PubMed CAS Google Scholar
28. Kashihara, M., Ueda, M., Horiguchi, Y., Furukawa, F., Hanaoka, M., and Imamura, S. (1986) A monoclonal antibody specifically reactive to human Langerhans cells. J. Invest. Dermatol., 87, 602–612.
    Article PubMed CAS Google Scholar
29. Valladeau, J., Ravel, O., Dezutter-Dambuyant, C., et al. (2000) Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules. Immunity 12, 71–81.
    Article PubMed CAS Google Scholar
30. Koch, F., Trockenbacher, B., Kämpgen, E., et al. (1995) Antigen processing in populations of mature murine dendritic cells is caused by subsets of incompletely matured cells. J. Immunol. 155, 93–100.
    PubMed CAS Google Scholar
31. Pierre, P., Turley, S. J., Gatti, E., et al. (1997) Developmental regulation of MHC class II transport in mouse dendritic cells. Nature, 388, 787–792.
    Article PubMed CAS Google Scholar
32. Kämpgen, E., Koch, N., Koch, F., et al. (1991) Class II major histocompatibility complex molecules of murine dendritic cells: synthesis, sialylation of invariant chain, and antigen processing capacity are down-regulated upon culture. Proc. Natl. Acad. Sci. USA, 88, 3014–3018.
    Article PubMed Google Scholar
33. Puré, E., Inaba, K., Crowley, M. T., et al. (1990) Antigen processing by epidermal Langerhans cells correlates with the level of biosynthesis of major histocompatibility complex class II molecules and expression of invariant chain. J. Exp. Med. 172, 1459–1469.
    Article PubMed Google Scholar
34. Becker, D., Reske-Kunz, A.B., Knop, J. and Reske, K. (1991) Biochemical properties of MHC class II molecules endogenously synthesized and expressed by mouse Langerhans cells. Eur.J. Immunol., 21,1213.
    Article PubMed CAS Google Scholar
35. Romani, N., Fritsch, P., and Schuler, G. (1991) Identification and phenotype of epidermal Langerhans cells. In Epidermal Langerhans cells, Schuler, G. (ed.). CRC Press, Boca Raton, FL., pp. 49–86.
    Google Scholar
36. Ortner, U., Inaba, K., Koch, F., et al. (1996) An improved isolation method for murine migratory cutaneous dendritic cells. J. Immunol. Methods, 193, 71–79.
    Article PubMed CAS Google Scholar
37. Lenz, A., Heine, M., Schuler, G., and Romani, N. (1993) Human and murine dermis contain dendritic cells. Isolation by means of a novel method and phenotypical and functional characterization. J. Clin. Invest., 92, 2587–2596.
    Article PubMed CAS Google Scholar
38. Weinlich, G., Heine, M., Stössel, H., et al. (1998) Entry into afferent lymphatics and maturation in situ of migrating cutaneous dendritic cells. J. Invest. Dermatol. 110, 441–448.
    Article PubMed CAS Google Scholar
39. Witmer-Pack, M. D., Valinsky, J., Olivier, W., and Steinman, R. M. (1988) Quantitation of surface antigens on cultured murine epidermal Langerhans cells: rapid and selective increase in the level of surface MHC products. J. Invest. Dermatol., 90, 387–394.
    Article PubMed CAS Google Scholar
40. Larsen C. P., Steinman, R. M., Witmer-Pack, M., Hankins, D. F., Morris, P. J. and Austyn, J. M. (1990) Migration and maturation of Langerhans cells in skin transplants and explants. J. Exp. Med. 172, 1483–1493.
    Article PubMed CAS Google Scholar

Download references