Manipulating Mouse Embryonic Stem Cells (original) (raw)

Abstract

In recent years, gene targeting technology has been rapidly evolving and has been used by a number of laboratories to systematically alter the mouse genome and to generate mice with specific targeted gene mutations. The improvement in the quality of reagents (embryonic stem (ES) cells and media) available to the scientific community has made this technology accessible to many laboratories.

Similar content being viewed by others

References

  1. Robertson, E. J. (ed.) (1987) Teratocarcinomas and Embryonic Stem Cells: A Practical Approach, IRL Press, Washington, DC.
    Google Scholar
  2. Hogan, B., Costantini, F., and Lacy, E. (eds.) (1986) Manipulating the Mouse Embryo: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York.
    Google Scholar
  3. Joyner, A. L. (Ed.) 1993. Gene Targeting: A Practical Approach, Oxford University Press, New York.
    Google Scholar
  4. Wassarman, P. M. and DePamphilis, M. L. (Eds.) 1993. Guide to techniques in mouse development, vol. 225 in: Methods in Enzymology, Academic Press, San Diego.
    Google Scholar
  5. Swiatek, P. J. and Gridley, T. (1993) Perinatal lethality and defects in hindbrain development in mice homozygous for a targeted mutation of the zinc finger gene Krox 20. Genes Dev. 11, 2071–2084.
    Article Google Scholar
  6. Sambrook, J., Fritsch, E. F., and Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, New York.
    Google Scholar
  7. Laird, P. W., Zijderveld, Linders, K., et al. (1991) Simplified mammalian DNA isolation procedure. Nuc. Acids Res. 19, 4293.
    Article CAS Google Scholar

Download references

Author information

Authors and Affiliations

  1. Neural Development Group, Mouse Cancer Genetics, Program, NCI-Frederick Cancer Research and Development Center, Frederick, MD
    Lino Tessarollo

Authors

  1. Lino Tessarollo
    You can also search for this author inPubMed Google Scholar

Editor information

Editors and Affiliations

  1. National Vision Research Institute of Australia, Carlton, Victoria, Australia
    Martin J. Tymms
  2. Centre for Functional Genomics and Human Disease, Institute of Reproduction and Development, Monash University, Clayton, Victoria, Australia
    Ismail Kola

Rights and permissions

© 2001 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Tessarollo, L. (2001). Manipulating Mouse Embryonic Stem Cells. In: Tymms, M.J., Kola, I. (eds) Gene Knockout Protocols. Methods in Molecular Biology, vol 158. Humana Press. https://doi.org/10.1385/1-59259-220-1:47

Download citation

Publish with us