High-Throughput TAIL-PCR as a Tool to Identify DNA Flanking Insertions (original) (raw)

References

1. Sessions, A., Burke, E., Presting, G., et al. (2002) A high-throughput Arabidopsis reverse genetics system. Plant Cell 14, 2985–2994.
   Article PubMed CAS Google Scholar
2. Parinov, S. and Sundaresan, V. (2000) Functional genomics in Arabidopsis: large-scale insertional mutagenesis complements the genome sequencing project. Curr. Opin. Biotechnol. 11, 157–161.
   Article PubMed CAS Google Scholar
3. Altschul, S. F., Madden, T. L., Schaffer, A. A., et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25, 3389–3402.
   Article PubMed CAS Google Scholar
4. Liu, Y. G. and Whittier, R. F. (1995) Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25, 674–681.
   Article PubMed CAS Google Scholar
5. Liu, Y. G., Mitsukawa, N., Oosumi, T., and Whittier, R. F. (1995) Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J. 8, 457–463.
   Article PubMed CAS Google Scholar
6. McElver, J., Tzafrir, I., Aux, G., et al. (2001) Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana. Genetics 159, 1751–1763.
   PubMed CAS Google Scholar
7. Budziszewski, G. J., Lewis, S. P., Glover, L. W., et al. (2001) Arabidopsis genes essential for seedling viability: isolation of insertional mutants and molecular cloning. Genetics 159, 1765–1778.
   PubMed CAS Google Scholar
8. Parinov, S., Sevugan, M., Ye, D., Yang, W.-C., Kumaran, M., and Sundaresan, V. (1999) Analysis of flanking sequences from dissociation insertion lines. A database for reverse genetics in Arabidopsis. Plant Cell 11, 2263–2270.
   Article PubMed CAS Google Scholar
9. Tsugeki, R., Kochieva, E. Z., and Fedoroff, N. V. (1996) A transposon insertion in the Arabidopsis SSR16 gene causes an embryo-defective lethal mutation. Plant J. 10, 479–489.
   Article PubMed CAS Google Scholar
10. Tissier, A. F., Marillonnet, S., Klimyuk, V., et al. (1999) Multiple independent defective Suppressor-mutator transposon insertions in Arabidopsis: a tool for functional genomics. Plant Cell 11, 1841–1852.
    Article PubMed CAS Google Scholar
11. Labarca, C. and Paigen, K. (1980) A simple, rapid, and sensitive DNA assay procedure. Anal. Biochem. 102, 344–352.
    Article PubMed CAS Google Scholar
12. Mazars, G.-R., Moyret, C., Jeanteur, P., and Theillet, C.-G. (1991) Direct sequencing by thermal asymmetric PCR. Nucleic Acids Res. 19, 4783.
    Article PubMed CAS Google Scholar
13. Gheysen, G., Herman, L., Breyne, P., Gielen, J., Van Montagu, M., and Depicker, A. (1990) Cloning and sequence analysis of truncated T-DNA inserts from Nicotiana tabacum. Gene 94, 155–163.
    Article PubMed CAS Google Scholar
14. Nacry, P., Camilleri, C., Courtial, B., Caboche, M., and Bouchez, D. (1998) Major chromosomal rearrangements induced by T-DNA transformation in Arabidopsis. Genetics 149, 641–650.
    PubMed CAS Google Scholar
15. De Neve, M., De Buck, S., Jacobs, A., Van Montagu, M., and Depicker, A. (1997) T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from cointegration of separate T-DNAs. Plant J. 11, 15–29.
    Article PubMed Google Scholar
16. Krizkova, L. and Hrouda, M. (1998) Direct repeats of T-DNA integrated in tobacco chromosome: characterization of junction regions. Plant J. 16, 673–680.
    Article PubMed CAS Google Scholar
17. De Buck, S., Jacobs, A., Van Montagu, M., and Depicker, A. (1999) The DNA sequences of T-DNA junctions suggest that complex T-DNA loci are formed by a recombination process resembling T-DNA integration. Plant J. 20, 295–304.
    Article PubMed Google Scholar
18. Ewing, B., Hillier, L., Wendl, M., and Green, P. (1998) Basecalling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res. 8, 175–185.
    PubMed CAS Google Scholar
19. Grossniklaus, U., Vielle-Calzada, J. P., Hoeppner, M. A., and Gagliano, W. B. (1998) Maternal control of embryogenesis by MEDEA, a polycomb group gene in Arabidopsis. Science 280, 446–450.
    Article PubMed CAS Google Scholar

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