Human Hepatocyte Culture (original) (raw)

References

1. Gebhardt, R. (1996) Special issue on: international congress on hepatocytes: applications in cell biology, toxicology and medicine. Cell Biol. Toxicol. 12, 1–65.
   Article Google Scholar
2. Maurel, P. (1996) The use of adult human hepatocytes in primary culture and other in vitro systems to investigate drug metabolism in man. Adv. Drug Deliv. Rev. 22, 105–132.
   Article CAS Google Scholar
3. Daujat, M., Pichard, L., Pineau, T., Fabre, G., Bonfils, C., and Maurel, P. (1991) Induction protocols for cytochromes P450IIIA in vivo and in primary cultures of animal and human hepatocytes. Methods Enzymol. 206, 345–353.
   Article CAS PubMed Google Scholar
4. Morel, F., Beaune, P., Ratanasavanh, D., et al. (1990) Expression of cytochrome P-450 enzymes in cultured human hepatocytes. Eur. J. Biochem. 91, 437–444.
   Article Google Scholar
5. Gomez-Lechon, M. J., Castell, J., Guillen, I., et al. (1995) Effects of hepatocyte growth factor on the growth and metabolism of human hepatocytes in primary culture. Hepatology 21, 1248–1254.
   Article CAS PubMed Google Scholar
6. Ismail, T., Howl, J., Wheatley, M., McMaster, P., Neuberger, J. M., and Strain, A. J. (1992) Growth of normal human hepatocytes in primary cultures: effect of hormones and growth factors on DNA synthesis. Hepatology 14, 1076–1082.
   Article Google Scholar
7. Kostrubsky, V. E., Strom, S. C., Wood, S. G., Wrighton, S. A., Sinclair, P. R., and Sinclair, J. F. (1995) Ethanol and isopentanol increase CYP3A and CYP2E in primary cultures of human hepatocytes. Arch. Biochem. Biophys. 322, 516–520.
   Article CAS PubMed Google Scholar
8. Li, A. P., Rasmussen, A., Xu, L., and Kaminski, D. L. (1995) Rifampicin induction of lidocaine metabolism in cultured human hepatocytes. J. Pharmacol. Exp. Ther. 274, 673–677.
   CAS PubMed Google Scholar
9. Berry, M. N. and Friend, D. S. (1969) High yield preparation of isolated rat liver parenchymal cells. J. Cell Biol. 43, 506–520.
   Article CAS PubMed PubMed Central Google Scholar
10. Seglen, P. O. (1976) Preparation of isolated rat liver cells. Methods Cell. Biol. 18, 29–83.
    Article Google Scholar
11. Isom, H. C. and Georgoff, I. (1984) Quantitative assay for albumin-producing liver cells after Simian virus 40 transformation of rat hepatocytes maintained in chemically defined medium. Proc. Natl. Acad. Sci USA 81, 6378–6382.
    Article CAS PubMed PubMed Central Google Scholar
12. Lanford, R. E., Carey, K. D., Estlack, L. E., Smith, G. C., and Hay, R. V. (1989) Analysis of plasma protein and lipoprotein synthesis in long-term primary cultures of baboon hepatocytes maintained in serum-free medium. In Vitro Cell. Dev. Biol. 25, 174–182.
    Article CAS PubMed Google Scholar
13. Diaz, D., Fabre, I., Daujat, M., et al. (1990) Omeprazole is an aryl hydrocarbon-like inducer of human hepatic cytochrome P450. Gastroenterology 99, 737–747.
    Article CAS PubMed Google Scholar
14. Ferrini, J. B., Pichard, L., Domergue, J., and Maurel, P. (1997) Long-term primary cultures of adult human hepatocytes. Chemico-Biol. Int. 107, 31–45.
    Article CAS Google Scholar
15. Greuet, J., Pichard, L., Ourlin, J. C., et al. (1997) Effect of cell density and epidermal growth factor on the inducible expression of CY3A and CYP1A genes in human hepatocytes in primary culture. Hepatology 25, 1166–1175.
    Article CAS PubMed Google Scholar
16. Pichard, L., Fabre, I., Daujat, M., Domergue, J., Joyeux, H., and Maurel, P. (1992) Effect of corticosteroids on the expression of cytochromes P450 and on cyclosporin A oxidase activity in primary cultures of human hepatocytes. Mol. Pharmacol. 41, 1047–1055.
    CAS PubMed Google Scholar
17. Curi-Pedrosa, R., Daujat, M., Pichard, L., et al. (1994) Omeprazole and lansoprazole are mixed inducers of CYP1A and CYP3A in human hepatocytes in primary culture. J. Pharmacol. Exp. Ther. 269, 384–392.
    CAS PubMed Google Scholar
18. Pascussi, J. M., Drocourt, L., Gerbal-Chaloin, S., Fabre, J. M., Maurel, P., and Vilarem, M. J. (2001) Dual effect of dexamethasone on CYP3A4 gene expression in human hepatocytes: sequential role of glucocorticoid receptor and pregnane X receptor. Eur. J. Biochem. 268, 6346–6357.
    Article CAS PubMed Google Scholar
19. Pascussi, J. M., Drocourt, L., Fabre, J. M., Maurel, P., and Vilarem, M. J. (2000) Dexamethasone induces pregnane X receptor and retinoid X receptor-α expression in human hepatocytes: synergistic increase of CYP3A4 induction by pregnane X receptor activators. Mol. Pharmacol. 58, 361–372.
    CAS PubMed Google Scholar
20. Pascussi, J. M., Gerbal-Chaloin, S., Fabre, J. M., Maurel, P., and Vilarem, M. J. (2000) Dexamethasone enhances CAR expression in human hepatocytes: consequences on CYP gene regulation. Mol. Pharmacol. 58, 1441–1450.
    CAS PubMed Google Scholar
21. Gerbal-Chaloin, S., Daujat, M., Pascussi, J. M., Pichard-Garcia, L., Vilarem, M. J., and Maurel, P. (2002) Transcriptional regulation of CYP2C9 gene: role of glucocorticoid receptor and constitutive androstane receptor. J. Biol. Chem. 277, 209–217.
    Article CAS PubMed Google Scholar
22. Drocourt, L., Ourlin, J. C., Pascussi, J. M. Fabre, J. M., Maurel, P., and Vilarem, M. J. (2002) Expression of CYP3A4, CYP2B6 and CYP2C9 is regulated by the vitamin D receptor pathway in primary human hepatocytes. J. Biol. Chem. 277, 25,125–25,132.
    Article CAS PubMed Google Scholar
23. Pichard-Garcia, L., Gerbal-Chaloin, S., Ferrini, J. B., Fabre, J. M., and Maurel, P. (2002) Use of long-term cultures of human hepatocytes to study CYP gene expression. Methods Enzymol. 357, 311–321
    Article CAS PubMed Google Scholar
24. Ourlin, J. C., Lasserre, F., Pineau, T., et al. (2003) The small heterodimer partner interacts with the pregnane X receptor and represses its transcriptional activity. Mol. Endocrinol. 17, 1693–703
    Article CAS PubMed Google Scholar
25. Pichard, L., Fabre, I., Fabre, G., et al. (1990) Cyclosporin A drug interactions: screening for inducers and inhibitors of cytochrome P-450 (cyclosporin A oxidase) in primary cultures of human hepatocytes and in liver microsomes. Drug Metab. Dispos. 18, 595–606.
    CAS PubMed Google Scholar
26. Maurice, M., Pichard, L., Daujat, M., et al. (1992) Effects of imidazole derivatives on cytochromes P450 from human hepatocytes in primary culture. FASEB J. 6, 752–758.
    CAS PubMed Google Scholar
27. Pichard-Garcia, L., Weaver, R. J., Eckett, N., et al. (2004) The olivacine derivatives 16020 (9-hydroxy-5,6-dimethyl-N-[2-(dimethylamino)ethyl)-6H-pyrido(4,3-B)-carbazole-1-carboxamide) induces CYP1A and its own metabolism in human hepatocytes in primary culture. _Drug Metab. Dispos._ **32**, 80–88
    Article CAS PubMed Google Scholar
28. Muntane-Relat, J., Ourlin, J. C., Domergue, J., and Maurel, P. (1995) Differential effects of cytokines on the inducible expression of CYP1A1, CYP1A2, and CYP3A4 in human hepatocytes in primary culture. Hepatology 22, 1143–1153.
    Article CAS PubMed Google Scholar
29. Biron-Andréani, C., Bezat-Bouchahda, C., Raulet, E., et al. (2004) Secretion of functional plasma haemostasis proteins in long-term primary cultures of human hepatocytes. Br. J. Haematol. 125, 638–646.
    Article PubMed Google Scholar
30. Ferrini, J. B., Rodrigues, E., Dulic, V., et al. (2001) Expression and DNA-binding activity of C/EBPa and C/EBPb in human liver and differentiated primary hepatocytes. J. Hepatol. 35, 170–77.
    Article CAS PubMed Google Scholar
31. Blanc, P., Etienne, H., Daujat, M., et al. (1991) Antiproliferative effect of FK506 and cyclosporin on adult human hepatocytes in culture. Transplant. Proc. 23, 2821–2824.
    CAS PubMed Google Scholar
32. Blanc, P., Etienne, H., Daujat, M., et al. (1992) Mitotic responsiveness of cultured adult human hepatocytes to epidermal growth factor, transforming growth factor a, and human serum. Gastroenterology 102, 1340–1350.
    CAS PubMed Google Scholar
33. Blanc, P., Liautard, J., Greuet, J., et al. (1995) Effect of anti-ulcer drugs on DNA synthesis in adult normal human hepatocytes in culture. Hepatology 22, 814–819.
    CAS PubMed Google Scholar
34. Fournier, C., Sureau, C., Coste, J., et al. (1998) In vitro infection of adult normal human hepatocytes in primary culture by hepatitis C virus. J. Gen. Virol. 79, 2367–2374.
    Article CAS PubMed Google Scholar
35. Castet, V., Fournier, C., Soulier, A., et al. (2002) Interferon-α inhibits hepatitis C virus replication in primary human hepatocytes infected in vitro. J. Virol. 76, 8189–8199.
    Article CAS PubMed PubMed Central Google Scholar
36. Sureau, C., Fournier-Wirth, C., and Maurel, P. (2003) Role of N-glycosylation of hepatitis B virus envelope in morphogenesis and infectivity of hepatitis delta virus. J. Virol. 77, 5519–5523
    Article CAS PubMed PubMed Central Google Scholar

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