Methods to Assess Tissue Permeability (original) (raw)

Abstract

An essential requirement for adequate organ performance is the formation of permeability barriers that separate and maintain compartments of distinctive structure. The endothelial cell lining of the vasculature defines a semipermeable barrier between the blood and the interstitial spaces of all organs. Disruption of the endothelial cell barrier can result in increased permeability and vascular leak. These effects are associated with multiple systemic disease states. The mechanisms that control barrier function are complex and their full understanding requires a multidisciplinary approach. In vivo permeability data often complement molecular findings and add power to the studies. The interaction of multiple cell types and tissues present only on mammalian models allow for testing of hypothesis and to establish the physiological significance of the results. In this chapter, we describe methods that can be used systematically to measure the permeability characteristics of several organs.

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References

  1. Beck, K. F., Eberhardt, W., Frank, S., et al. (1999) Inducible NO synthase: role in cellular signaling. J. Exp. Biol. 202, 645–653.
    CAS PubMed Google Scholar
  2. Bertuglia, S. and Giusti, A. (2005) Role of nitric oxide in capillary perfusion and oxygen delivery regulation during systemic hypoxia. Am. J. Physiol. Heart Circ. Physiol. 288, H525–H531.
    Article CAS PubMed Google Scholar
  3. Hofmann, T., Stutts, M. J., Ziersch, A., et al. (1998) Effects of topically delivered benzamil and amilorideon nasal potential difference in cystic fibrosis. Am. J. Resp. Crit. Care Med. 157, 1844–1849.
    CAS PubMed Google Scholar
  4. Karhausen, J., Ibla, J. C., and Colgan, S. P. (2003). Implications of hypoxia on mucosal barrier function. Cell. Mol. Biol. 49, 77–87.
    CAS PubMed Google Scholar
  5. Bleeker-Rovers, C. P., Boerman, O. C., Rennen, H. J., et al. (2004) Radiolabeled compounds in diagnosis of infectious and inflammatory disease. Curr. Pharm. Design. 10, 2935–2950.
    Article CAS Google Scholar
  6. Miles, A. A. and Miles, E. M. (1952) Vascular reactions to histamine, histamineliberator, and leukotaxine in the skin of guinea pigs. J. Physiol. (London) 118, 228–257.
    CAS Google Scholar
  7. Rudolph, A. M. and Heymann, M. A. (1967) The circulation of the fetus in utero. Methods for studying distribution of blood flow, cardiac output and organ blood flow. Circ. Res. 21, 163–184.
    CAS PubMed Google Scholar
  8. Synnestvedt, K., Furuta, G. T., Comeford, K. M., et al. (2002) Ecto-5’-nucleotidase (CD 73) regulation by hypoxia-inducible factor-1 (HIF-1) mediates permeability changes in intestinal epithelia. J. Clin. Invest. 110, 993–1002.
    CAS PubMed Google Scholar
  9. Samel, S., Keese, M., Kleczka, M., et al. (2002) Microscopy of bacterial translocation during small bowel obstruction and ischemia in vivo—a new animal model. BMC Surg. 2, 6.
    Article PubMed Google Scholar
  10. Sorrells, D. L., Friend, C., Koltuksuz, U., et al. (1996) Inhibition of nitric oxide with aminoguanidine reduces bacterial translocation after endotoxin challenge in vivo. Arch. Surg. 131, 1155–1163.
    CAS PubMed Google Scholar
  11. Thompson, L. F., Eltzschig, H. K., Ibla, J. C., et al. (2004) Critical role for the ecto-5’-nucleotidase (CD73) in vascular leak during hypoxia. J. Exp. Med. 200, 1395–1405.
    Article CAS PubMed Google Scholar
  12. Toung, T. J., Chang, Y., Lin, J., and Bhardwaj, A. (2005) Increases in lung and brain water following experimental stroke: effect of mannitol and hypertonic saline. Crit. Care Med. 33, 203–208; discussion 259–260.
    Article CAS PubMed Google Scholar

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Authors and Affiliations

  1. Department of Anesthesiology, Perioperative, and Pain Medicine, Center for Experimental Therapeutics and Reperfusion Injury, Brigham
    Juan C. Ibla & Joseph Khoury
  2. Women’s Hospital, Children’s Hospital Boston, Harvard Medical School, Boston, MA
    Juan C. Ibla & Joseph Khoury

Authors

  1. Juan C. Ibla
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  2. Joseph Khoury
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Editor information

Editors and Affiliations

  1. Center for Experimental Therapeutics and Reperfusion Injury, Brigham
    Sean P. Colgan
  2. Women’s Hospital, Havard Medical School, Boston, MA, USA
    Sean P. Colgan

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© 2006 Humana Press Inc.

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Ibla, J.C., Khoury, J. (2006). Methods to Assess Tissue Permeability. In: Colgan, S.P. (eds) Cell-Cell Interactions. Methods in Molecular Biology™, vol 341. Humana Press. https://doi.org/10.1385/1-59745-113-4:111

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