Prevalence and molecular subtyping of Blastocystis sp. in rabbits in Henan, Central China (original) (raw)

Folia Parasitologica 69:027 (2022) | DOI: 10.14411/fp.2022.027

Changwei Su ORCID...1, 4, Xuefang Mei1, 4*, Xia Feng1, Fuqiang Zhang1, Pei Wang1, Bo He1, Fuyang Xu1, Zishan Yang2, Xiaowei Tian1, Zhenchao Zhang1, Xiangrui Li1, 3, Shuai Wang ORCID...1, *

1Xinxiang Key Laboratory of Pathogenic Biology, Department of Pathogenic Biology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan, PR China;

2Department of Immunology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan, PR China;

3MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, PR China

4These authors contributed equally

Species of Blastocystis Alexieff, 1911 are anaerobic intestinal protists found in humans and many kinds of animals that mainly cause diarrhea, abdominal pain and other clinical symptoms. At present, data on the prevalence and subtype diversity of species of Blastocystis in domestic rabbits are very limited. The purpose of this study was to characterise the infection rate and gene subtype distribution of Blastocystis sp. in domestic rabbits in Henan Province, Central China, and provide foundation for prevention and control of the disease caused by Blastocystis sp. in domestic rabbits. DNA was extracted from 286 fresh rabbit faecal samples collected from four areas of Henan Province, Central China. All DNA samples were screened using PCR and positive samples were sequenced to identify individual subtypes based on the small ribosomal subunit (SSU rRNA) gene. The overall infection rate of Blastocystis sp. in domestic rabbits in Henan Province was 15% (43/286). Three subtypes were identified, including ST1 (26/43, 60%), ST3 (5/43, 12%) and ST7 (12/43, 28%), all of which belonged to potentially zoonotic subtypes, ST1 was the dominant gene subtype. These results showed that infection with Blastocystis sp. was common in domestic rabbits in Henan Province, Central China, and was represented by zoonotic subtypes. Therefore, special attention should be paid to reduce the risk of transmission of Blastocystis sp. from domestic rabbits to humans.

Keywords: Blastocystis sp., SSU rRNA, epidemiological characteristics, gene subtype, rabbits

Received: January 18, 2022; Revised: June 23, 2022; Accepted: August 2, 2022; Published online: November 21, 2022 Show citation

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Su, C., Mei, X., Feng, X., Zhang, F., Wang, P., He, B., ... Wang, S. (2022). Prevalence and molecular subtyping of Blastocystis sp. in rabbits in Henan, Central China. Folia Parasitologica,�69,�Article 2022.027. https://doi.org/10.14411/fp.2022.027

ERRATUM: Su C., Mei X., Feng X., Zhang F., Wang P., He B., Xu F., Yang Z., Tian X., Zhang Z., Li X., Wang S. 2022: Prevalence and molecular subtyping of Blastocystis sp. in rabbits in Henan, Central China. Folia Parasitol. 70: 013, published in June 2023; doi: 10.14411/fp.2023.013

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