High fat diet-induced estrus cycle disruption: effects of Ficus asperifolia (original) (raw)

Abstract

Background: Ficus asperifolia (L) Hook. Ex Miq (Moraceae) fruits are used in Cameroonian traditional medicine to cure some cases of infertility in women. This study determines the mechanism of alleviating effect of the plant extracts on rat infertility induced by a high fat diet (HFD).

Methods: Obesity was reached by feeding female rats with a HFD for 10 weeks. Vaginal smear was observed daily for 3 weeks after animals were obese. Then, 70 animals with abnormal estrus cyclicity were selected and partitioned into two sets of 35 animals. Each set was further divided into seven groups of five rats. These obese rats with disrupted estrus cyclicity were orally administered the aqueous and methanolic extracts (100 and 500 mg/kg), distilled water (10 mL/kg), 5% Tween 80 (10 mL/kg) or lutenyl (0.8 µg/kg) once a day for 1 week (set I) or 4 weeks (set II). Estrus cyclicity, body weight gain, hematocrit, lipid profile, ovarian, uterine and hepatic growth indices were determined at the end of each treatment.

Results: HFD increased the body weight of the animals by 27% and disrupted the estrus cyclicity by 98.44%. Aqueous extract (100 mg/kg) of F. asperifolia given for 1 week corrected 40% of the irregular estrus cycle and this percentage increased to 80% as the treatment progressed to 4 weeks. _F. asperifolia_-treated obese rats (mostly with the aqueous extract at 100 mg/kg) showed a significant decrease (p<0.001) in the total plasma cholesterol and low density lipoprotein (LDL) cholesterol level and a significant increase (p<0.001) in high density lipoprotein (HDL) cholesterol. F. asperifolia has bioactive agents that may maintain conducive conditions for reproduction in obese female rats.

Conclusions: Our data support the anecdotal claims of F. asperifolia in folk medicine to cure some cases of infertility in women.

Acknowledgments

The authors are grateful to the Academy of Sciences for the Developing World (TWAS) for the grant [Ref 07–300 RG/BIO/AF/AC; UNESCOFR: 3240184286].

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Research funding: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

References

1. SchilloKK. Effects of dietary energy on control of luteinizing hormone secretion in cattle and sheep. J Anim Sci1992;70:1271–82.10.2527/1992.7041271xSearch in Google Scholar PubMed

2. TortorielloDV, McMinnJ, ChuaSC. Dietary-induced obesity and hypothalamic infertility in female DBA/2j mice. Endocrinology2004;145:1238–47.10.1210/en.2003-1406Search in Google Scholar PubMed

3. AkamineEH, MarçalAC, CamporezJP, HoshidaMS, CaperutoLC, BevilacquaE, et al. Obesity induced by high-fat diet promotes insulin resistance in the ovary. J Endocrinol2010;206:65–74.10.1677/JOE-09-0461Search in Google Scholar PubMed

4. QuennellJH, HowellCS, RoaJ, AugustineRA, GrattanDR, AndersonGM. Leptin deficiency and diet-induced obesity reduce hypothalamic kisspeptin expression in mice. Endocrinology2011;152:1541–50.10.1210/en.2010-1100Search in Google Scholar PubMed PubMed Central

5. WuS, DivallS, WondisfordF, WolfeA. Reproductive tissues maintain insulin sensitivity in diet-induced obesity. Diabetes2012;61:114–23.10.2337/db11-0956Search in Google Scholar PubMed PubMed Central

6. CarrieLMB, OlsterD. Abnormal estrus cyclicity and behavioral hyporesponsiveness to ovarian hormones in genetically obese Zucker female rats. Endocrinology1997;138:143–8.10.1210/endo.138.1.4849Search in Google Scholar PubMed

7. FullstonT, PalmerNO, OwensJA, MitchellM, BakosHW, LaneM. Diet-induced paternal obesity in the absence of diabetes diminishes the reproductive health of two subsequent generations of mice. Hum Reprod2012;27:1391–400.10.1093/humrep/des030Search in Google Scholar PubMed

8. Gil-CamposM, CaneteR, GilA. Hormones regulating lipid metabolism and plasma lipids in childhood obesity. Anim Sci2004;28:575–80.10.1038/sj.ijo.0802806Search in Google Scholar PubMed

9. LuzzoKM, WangQ, PurcellSH, ChiM, JimenezPT,GrindlerN, et al. High fat diet induced developmental defects in the mouse: oocyte meiotic aneuploidy and fetal growth retardation/brain defects. PLoS One2012;7:49217.10.1371/journal.pone.0049217Search in Google Scholar PubMed PubMed Central

10. Bermejo-AlvarezP, RosenfeldCS, RobertsRM. Effect of maternal obesity on estrus cyclicity, embryo development and blastocyst gene expression in a mouse model. Hum Reprod2012;27:3513–22.10.1093/humrep/des327Search in Google Scholar PubMed PubMed Central

11. BinderNK, HannanNJ, GardnerDK. Paternal diet-induced obesity retards early mouse embryo development, mitochondrial activity and pregnancy health. Plos One2012;7:52304.10.1371/journal.pone.0052304Search in Google Scholar

12. SalahAM, GathumbiJ, VierlingW, WagnerH. Estrogenic and cholinergic properties of the methanol extract of Ruellia praetermissa Sceinf. Ex. Lindau (Acantaceae) in female rats. Phytomedicine2002;9:52–5.10.1078/0944-7113-00079Search in Google Scholar

13. HongL,GuoZ,HuangK,WeiS,LongC.Ethnobotanical study on medicinal plants used by Maonan people in China. J Ethnobiol Ethnomed2015;11(1):32–9.10.1186/s13002-015-0019-1Search in Google Scholar

14. AdjanohounE, AhiyiMR, AkeAL, DramaneK, ElewadeJA, FadajuSA, et al. Traditional medicine and pharmacopoeia, contribution to ethnobotanical and floristic studies in Western Nigeria. Paris: Edition Marketing, 1991:119.Search in Google Scholar

15. WatchoP, NgadjuiE, Nkeng-efouetPA, NguelefackTB, KamanyiA. Reproductive effects of Ficus asperifolia (Moraceae) in female rats. African Health Sci2009;9:49–53.Search in Google Scholar

16. EstherN, PierreW, TelesphoreBN, AlbertK. Effects of Ficus asperifolia on normal rat estrus cyclicity. Asian Pac J Trop Biomed2013;3:53–7.10.1016/S2221-1691(13)60023-4Search in Google Scholar

17. WatchoP, NgadjuiE, Nkeng-efouetPA, NguelefackTB, KamanyiA. Evaluation of in vitro uterotonic activities of fruit extracts of Ficus asperifolia in rats. ECAM2010;21:1–7.10.1093/ecam/nep221Search in Google Scholar PubMed PubMed Central

18. DeS, DeyYN, GhoshAK. Phytochemical investigation and chromatographic evaluation of the different extracts of tuber of Amorphophallus paeoniifolius (Araceae). Int J Pharm Biomed Res2010;1:150–7.Search in Google Scholar

19. SharpPE, La ReginaMC. The laboratory rat. CRC Press, Inc., Boca Raton, FL. 23, 2009.Search in Google Scholar

20. FriedewaldWT, LevyRI, FredricksonDS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem1972;18:499–502.10.1093/clinchem/18.6.499Search in Google Scholar

21. DoHJ, JinT, ChungJH, HwangJW, ShinMJ. Voglibose administration regulates body weight and energy intake in high fat-induced obese mice. Biochem Biophys Res Commun2014;291:2191–21918.Search in Google Scholar

22. MustaphaAR, BawaEK, OgwuD, AbdullahiUS, KaikaboAA, DiarraSS. Effects of ethanolic extract of Rhynchosia sublobata (Schumach) Meikle on estrus cycle in Wistar rats. Int J Med Arom2011;1:122–7.Search in Google Scholar

23. PanidisD, TziomalosK, MisicronisG, PapadakisE, BetsasG, KatsikisI, et al. Insulin resistance and endocrine characteristics of the different phenotypes of polycystic ovary syndrome: a prospective study. Hum Reprod2012;27:541–9.10.1093/humrep/der418Search in Google Scholar PubMed

24. BeckKD, JiaoX, CominskiTP, ServatiusRJ. Estrus cycle stage modifies the presentation of stress-induced startle suppression in female Sprague-Dawley rats. Physiol Beh2008;93:1019–23.10.1016/j.physbeh.2008.01.012Search in Google Scholar PubMed

25. WeshoodFR. The reproductive cycle; a practical histological guide to staging. Toxicol Pathol2008;36:375–84.10.1177/0192623308315665Search in Google Scholar PubMed

26. ByersSI, WilesMV, DunnSL, TaftRA. Mouse estrous cycle identification tool and images. Plos One2012;7:35538.10.1371/journal.pone.0035538Search in Google Scholar PubMed PubMed Central

27. GalvanM, UauyR, CorvalanC, Lopez-RodríguezG, KainJ. Determinants of cognitive development of low SES children in Chile: a post-transitional country with rising childhood obesity rates. Matern Child Health J2013;17:1243–51.10.1007/s10995-012-1121-9Search in Google Scholar PubMed

28. FaroogR, LutfullahS, AhmedM. Serum leptin levels in obese infertile men and women. Pak J Pharm Sci2014;27:67–71.Search in Google Scholar

29. WhiteJT, DesantoCL, GibbonsC, LardnerCK, PanakosA, RaisS, et al. Insulins, leptin and feeding in a population of Peromyscus leucopus (White-footed mouse) with variable fertility. Horm Behay2014;506:28–30.Search in Google Scholar

30. PantasriT, NormanRJ. The effects of being overweight and obese on female reproduction: a review. Gynecol Endocrinol2014;30:90–4.10.3109/09513590.2013.850660Search in Google Scholar PubMed

31. SunmanH, CanpolatU, SahinerL, AytemirK. Use of fenofibrate during the first trimester of unplanned pregnancy in a patient with hypertriglyceridemia. Ann Pharmacother2012;46:5–8.10.1345/aph.1Q626Search in Google Scholar PubMed

32. WathesDC. Mechanisms linking metabolic status and disease whit reproductive outcome in the dairy cow. Reprod Domest Anim2012;47:304–12.10.1111/j.1439-0531.2012.02090.xSearch in Google Scholar PubMed

33. WongNC. The beneficial effects of plant sterols on serum cholesterol. Can J Cardiol2001;17:715–19.Search in Google Scholar