Y-SNP Typing of U.S. African American and Caucasian Samples Using Allele-Specific Hybridization and Primer Extension (original) (raw)
Research-Article
1
Biotechnology Division, National Institute of Standards and Technology
,
Gaithersburg, MD 20899-8311
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1
Biotechnology Division, National Institute of Standards and Technology
,
Gaithersburg, MD 20899-8311
Search for other works by this author on:
PM Vallone, Ph.D.
1
Biotechnology Division, National Institute of Standards and Technology
,
Gaithersburg, MD 20899-8311
JM Butler, Ph.D.
1
Biotechnology Division, National Institute of Standards and Technology
,
Gaithersburg, MD 20899-8311
J. Forensic Sci.. Jul 2004, 49(4): 1-10 (10 pages)
Published Online: July 1, 2004
Abstract
Multiplex analysis of genetic markers has become increasingly important in a number of fields, including DNA diagnostics and human identity testing. Two methods for examination of single nucleotide polymorphisms (SNPs) with a potential for a high degree of multiplex analysis of markers are primer extension with fluorescence detection, and allele-specific hybridization using flow cytometry. In this paper, we examined 50 different SNPs on the Y-chromosome using three primer extension multiplexes and five hybridization multiplex assays. For certain loci, the allele-specific hybridization method exhibited sizable background signal from the absent alternate allele. However, 100% concordance (>2000 alleles) was observed in ten markers that were typed using both methods. A total of 18 unique haplogroups out of a possible 45 were observed in a group of 229 U.S. African American and Caucasian males with the majority of samples being assigned into 2 of the 18 haplogroups.
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