Overexpression of the transcription factor Yap1 modifies intracellular redox conditions and enhances recombinant protein secretion (original) (raw)

Affiliations:

1 Department of Biotechnology, BOKU University of Natural Resources and Life Sciences Vienna, Vienna, Austria.

2 Austrian Centre of Industrial Biotechnology (ACIB), Vienna, Austria.

3 School of Bioengineering, University of Applied Sciences FH Campus Wien, Vienna, Austria.

4 Department of Chemistry, BOKU University of Natural Resources and Life Sciences Vienna, Vienna, Austria.

Keywords:

ER, cytosol, cellular redox regulation, oxidative protein folding, glutathione, redox sensitive roGFP, Pichia pastoris.

Abbreviations:

ER - endoplasmic reticulum,

PDI - protein disulfide isomerase,

ROS - reactive oxygen species,

roGFP - redox sensitive variants of green fluorescent protein,

SOD - superoxide dismutase,

TRP - trypsinogen,

UPR - unfolded protein response.

Corresponding Author(s):

Brigitte Gasser, Department of Biotechnology, BOKU University of Natural Resources and Life Sciences Vienna, Muthgasse 18; 1190 Vienna, Austriabrigitte.gasser@boku.ac.at

Conflict of interest statement:

The authors declare no conflict of interest.

Please cite this article as:

Marizela Delic, Alexandra B. Graf, Gunda Koellensperger, Christina Haberhauer-Troyer, Stephan Hann, Diethard Mattanovich, Brigitte Gasser (2014). Overexpression of the transcription factor Yap1 modifies intracellular redox conditions and enhances recombinant protein secretion. Microbial Cell 1(11): 376-386.

© 2014 Delic et al. This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.