Gene Expression Signatures of Seven Individual Human Embryonic Stem Cell Lines (original) (raw)

Journal Article

,

Turku Centre for Biotechnology, University of Turku and Åbo Akademi University

, Turku,

Finland

Department of Clinical Science, Karolinska Institutet, Karolinska University Hospital Huddinge

, Stockholm,

Sweden

Correspondence: Heli Skottman, Ph.D., Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, POB 123, 0520 Turku, Finland. Telephone: 358‐02‐3338622; Fax: 358‐02‐3338000; e-mail: Heli.Skottman@btk.fi

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Biomedicum Helsinki, Program of Developmental and Reproductive Biology, University of Helsinki

, Helsinki,

Finland

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Biomedicum Helsinki, Program of Developmental and Reproductive Biology, University of Helsinki

, Helsinki,

Finland

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Biomedicum Helsinki, Program of Developmental and Reproductive Biology, University of Helsinki

, Helsinki,

Finland

Family Federation of Finland

, Helsinki,

Finland

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Department of Clinical Science, Karolinska Institutet, Karolinska University Hospital Huddinge

, Stockholm,

Sweden

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Biomedicum Helsinki, Program of Developmental and Reproductive Biology, University of Helsinki

, Helsinki,

Finland

Family Federation of Finland

, Helsinki,

Finland

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Biomedicum Helsinki, Program of Developmental and Reproductive Biology, University of Helsinki

, Helsinki,

Finland

Hospital for Children and Adolescents, University of Helsinki

, Helsinki,

Finland

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Department of Clinical Science, Karolinska Institutet, Karolinska University Hospital Huddinge

, Stockholm,

Sweden

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Turku Centre for Biotechnology, University of Turku and Åbo Akademi University

, Turku,

Finland

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Received:

02 December 2004

Published:

01 October 2005

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Heli Skottman, Milla Mikkola, Karolina Lundin, Cia Olsson, Anne‐Marie Strömberg, Timo Tuuri, Timo Otonkoski, Outi Hovatta, Riitta Lahesmaa, Gene Expression Signatures of Seven Individual Human Embryonic Stem Cell Lines, Stem Cells, Volume 23, Issue 9, October 2005, Pages 1343–1356, https://doi.org/10.1634/stemcells.2004-0341
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Abstract

Identification of molecular components that define a pluripotent human embryonic stem cell (hESC) provides the basis for understanding the molecular mechanisms regulating the maintenance of pluripotency and induction of differentiation. We compared the gene expression profiles of seven genetically independent hESC lines with those of nonlineage‐differentiated cells derived from each line. A total of 8,464 transcripts were expressed in all hESC lines. More than 45% of them have no yet‐known biological function, which indicates that a high number of unknown factors contribute to hESC pluripotency. Among these 8,464 transcripts, 280 genes were specific for hESCs and 219 genes were more than twofold differentially expressed in all hESC lines compared with nonlineage‐differentiated cells. They represent genes implicated in the maintenance of pluripotency and those involved in early differentiation. The chromosomal distribution of these hESC‐enriched genes showed over‐representation in chromosome 19 and under‐representation in chromosome 18. Although the overall gene expression profiles of the seven hESC lines were markedly similar, each line also had a subset of differentially expressed genes reflecting their genetic variation and possibly preferential differentiation potential. Limited overlap between gene expression profiles illustrates the importance of cross‐validation of results between different ESC lines.

Copyright © 2005 AlphaMed Press

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open\_access/funder\_policies/chorus/standard\_publication\_model)

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