Cell Cycle Features of Primate Embryonic Stem Cells (original) (raw)

Journal Article

,

Institut National de la Santé et de la Recherche Médicale (INSERM), U371, Cerveau et Vision, Department of Stem Cells and Cortical Development

, Bron,

France

Université Claude Bernard Lyon I, IFR19 Institut Fédératif des Neurosciences

, Bron,

France

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Institut National de la Santé et de la Recherche Médicale (INSERM), U371, Cerveau et Vision, Department of Stem Cells and Cortical Development

, Bron,

France

Université Claude Bernard Lyon I, IFR19 Institut Fédératif des Neurosciences

, Bron,

France

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,

Institut National de la Santé et de la Recherche Médicale (INSERM), U371, Cerveau et Vision, Department of Stem Cells and Cortical Development

, Bron,

France

Université Claude Bernard Lyon I, IFR19 Institut Fédératif des Neurosciences

, Bron,

France

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,

INSERM

, U412, Lyon,

France

Ecole Normale Supérieure de Lyon

, Lyon,

France

Université Claude Bernard Lyon I

, IFR128 BioSciences Lyon-Gerland, Lyon,

France

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INSERM

, U412, Lyon,

France

Ecole Normale Supérieure de Lyon

, Lyon,

France

Université Claude Bernard Lyon I

, IFR128 BioSciences Lyon-Gerland, Lyon,

France

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,

Oregon National Primate Research Center, Oregon Health & Science University

, Beaverton, Oregon,

USA

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Oregon National Primate Research Center, Oregon Health & Science University

, Beaverton, Oregon,

USA

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,

Institut National de la Santé et de la Recherche Médicale (INSERM), U371, Cerveau et Vision, Department of Stem Cells and Cortical Development

, Bron,

France

Université Claude Bernard Lyon I, IFR19 Institut Fédératif des Neurosciences

, Bron,

France

INSERM

, U371, PrimaStem, Bron,

France

Correspondence: Pierre Savatier, Ph.D., INSERM, U371, Cerveau et Vision, Department of Stem Cells and Cortical Development, 18 avenue Doyen Lépine, 69500 Bron, France. Telephone: +33 4 72 91 34 42; Fax: +33 4 72 91 34 61; e-mail: savatier@lyon.inserm.fr

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Institut National de la Santé et de la Recherche Médicale (INSERM), U371, Cerveau et Vision, Department of Stem Cells and Cortical Development

, Bron,

France

Université Claude Bernard Lyon I, IFR19 Institut Fédératif des Neurosciences

, Bron,

France

INSERM

, U371, PrimaStem, Bron,

France

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Accepted:

12 October 2005

Published:

20 October 2005

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Anne-Catherine Fluckiger, Guillaume Marcy, Mélanie Marchand, Didier Négre, François-Loïc Cosset, Shoukhrat Mitalipov, Don Wolf, Pierre Savatier, Colette Dehay, Cell Cycle Features of Primate Embryonic Stem Cells, Stem Cells, Volume 24, Issue 3, March 2006, Pages 547–556, https://doi.org/10.1634/stemcells.2005-0194
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Abstract

Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit through the G1 phase, which accounts for 15% of the total cell cycle duration. Monkey ESCs exhibit a non-phasic expression of cyclin E, which is detected during all phases of the cell cycle, and do not growth-arrest in G1 after γ-irradiation, reflecting the absence of a G1 checkpoint. Serum deprivation or pharmacological inhibition of mitogen-activated protein kinase kinase (MEK) did not result in any alteration in the cell cycle distribution, indicating that ESC growth does not rely on mitogenic signals transduced by the Ras/Raf/MEK pathway. Taken together, these data indicate that rhesus monkey ESCs, like their murine counterparts, exhibit unusual cell cycle features in which cell cycle control mechanisms operating during the G1 phase are reduced or absent.

Copyright © 2006 AlphaMed Press

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open\_access/funder\_policies/chorus/standard\_publication\_model)

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