Computerized Video Time-Lapse (CVTL) Analysis of Cell Death Kinetics in Human Bladder Carcinoma Cells (EJ30) X-Irradiated in Different Phases of the Cell Cycle (original) (raw)
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1 December 2002 Computerized Video Time-Lapse (CVTL) Analysis of Cell Death Kinetics in Human Bladder Carcinoma Cells (EJ30) X-Irradiated in Different Phases of the Cell Cycle
Kenneth Chu,Edith A. Leonhardt,Maxine Trinh,Geraldine Prieur-Carrillo,Johan Lindqvist,Norman Albright,C. Clifton Ling,William C. Dewey
Author Affiliations +
Kenneth Chu,1 Edith A. Leonhardt,1 Maxine Trinh,1 Geraldine Prieur-Carrillo,1 Johan Lindqvist,1 Norman Albright,1 C. Clifton Ling,2 William C. Dewey1,*
1aRadiation Oncology Research Laboratory, University of California San Francisco, MCB 200, 1855 Folso
2b bDepartment of Medical Physics, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York
*Author to whom correspondence should be addressed at Radiation Oncology Research Laboratory, University of California San Francisco, MCB 200, 1855 Folsom Street, San Francisco, CA 94103; dewey@rorl.ucsf.edu
Abstract
Chu, K., Leonhardt, E. A., Trinh, M., Prieur-Carrillo, G., Lindqvist, J., Albright, N., Ling, C. C. and Dewey, W. C. Computerized Video Time-Lapse (CVTL) Analysis of Cell Death Kinetics in Human Bladder Carcinoma Cells (EJ30) X-Irradiated in Different Phases of the Cell Cycle. Radiat. Res. 158, 667–677 (2002).
The purpose of this study was to quantify the modes and kinetics of cell death for EJ30 human bladder carcinoma cells irradiated in different phases of the cell cycle. Asynchronous human bladder carcinoma cells were observed in multiple fields by computerized video time-lapse (CVTL) microscopy for one to two cell divisions before irradiation (6 Gy) and for 6–11 days afterward. By analyzing time-lapse movies collected from these fields, pedigrees were constructed showing the behaviors of 231 cells irradiated in different phases of the cell cycle (i.e. at different times after mitosis). A total of 219 irradiated cells were determined to be non-colony-forming over the time spans of the experiments. In these nonclonogenic pedigrees, cells died primarily by necrosis either without entering mitosis or over 1 to 10 postirradiation generations. A total of 105 giant cells developed from the irradiated cells or their progeny, and 30% (31/105) divided successfully. Most nonclonogenic cells irradiated in mid-S phase (9–12 h after mitosis) died by the second generation, while those irradiated either before or after this short period in mid-S phase had cell deaths occurring over one to nine postirradiation generations. The nonclonogenic cells irradiated in mid-S phase also experienced the longest average delay before their first division. Clonogenic cells (11/12 cells) divided sooner after irradiation than the average nonclonogenic cells derived from the same phase of the cell cycle. The early death and long division delay observed for nonclonogenic cells irradiated in mid-S phase could possibly result from an increase in damage induced during the transition from the replication of euchromatin to the replication of heterochromatin.
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Kenneth Chu, Edith A. Leonhardt, Maxine Trinh, Geraldine Prieur-Carrillo, Johan Lindqvist, Norman Albright, C. Clifton Ling, and William C. Dewey "Computerized Video Time-Lapse (CVTL) Analysis of Cell Death Kinetics in Human Bladder Carcinoma Cells (EJ30) X-Irradiated in Different Phases of the Cell Cycle," Radiation Research 158(6), 667-677, (1 December 2002). https://doi.org/10.1667/0033-7587(2002)158[0667:CVTLCA]2.0.CO;2
Received: 18 December 2002; Accepted: 1 August 2002; Published: 1 December 2002
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