JLE - European Journal of Dermatology (original) (raw)

Illustrations

Figure 1

Dense infiltration of eosinophils in the superficial and deep perivascular dermis, and subcutaneous layer (A). A paraffin-embedded tissue sample was deparaffinized and stained using anti-CD163 Ab (B), anti-CD206 Ab (C) or anti-Foxp3 Ab (D), and developed with liquid permanent red. (Original magnification x400 (A, D) × 200 (B, C)).

Figure 1

Figure 2

M2 macrophages induced from PBMC from healthy donors were cultured for 5 days and stimulated by IL-4 or IL-13 for 48 hours. The cultured cells were harvested from the culture and their cytokine-producing pattern was examined by flow cytometry after intracellular staining with FITC-conjugated anti-Arg1.

Figure 2

Figure 3

M2 macrophages from PBMCs were stimulated by IL-4 or IL-13. Six hours after stimulation, total RNA was recovered from macrophages and then reverse transcribed into cDNA. Quantitative real-time PCR was conducted to determine the number of copies of cDNA for each chemokines, cytokine and Arg1, and relative mRNA expression levels were calculated for each gene and each time point after normalization against GAPDH, using the ΔΔCt method. The data from each donor were obtained by the triplicated assays and then the mean ± SD was calculated. One of the representative data from three different donors is shown.

Figure 3

Figure 4

The culture supernatants of M2 macrophages stimulated with IL-4 or IL-13 were collected at 48 h after culture. Their production of CCL17 (A) or CCL24 (B) was measured by ELISA. Representative data from at least three independent experiments are shown. A paraffin-embedded tissue sample was deparaffinized and stained using anti-CCL17 Ab (C) or anti-CCL24 Ab (D) and developed with liquid permanent red. (Original magnification × 200 (C, D)).

Figure 4

Auteurs

Department of Dermatology,
Tohoku University Graduate School of Medicine,
Seiryo-machi 1-1, Aoba-ku,
Sendai, 980-8574, Japan

* Reprints

Background: M2 macrophages play a critical role in the recruitment of T helper 2 (Th2) regulatory T cells (Treg). Objectives To study the role of M2 macrophages and Treg cells in eosinophilic celulitis. Material and Methods: We employed immunohistochemical staining for CD163 and CD206 (macrophages) as well as FoxP3 (Treg), in lesional skin of four cases of eosinophilic cellulitis. Results: CD163+ CD206+ M2 macrophages, which were previously reported to produce CCL17 to induce Th2 cells and Treg cells, were predominantly infiltrating the subcutaneous tissues and interstitial area of the dermis. M2 macrophages derived from PBMC showed significantly increased expression of CCL11, CCL17, CCL24 and CCL26 mRNA and production of CCL17 and CCL24, when stimulated by IL-4 or IL- 13. In addition, CCL17-producing cells and CCL24-producing cells were prominent in the lesional skin of EC. Conclusion: Our study sheds light on one of the possible immunological mechanisms of eosinophilic cellulitis.