Evaluation of Modified Formalin-Ether Concentration Method Using Para Tube in Clinical Settings (original) (raw)

Eun Jeong Won, M.D.1, Jin Kim, M.D.2, Dong Wook Ryang, M.D.1

Department of Laboratory Medicine1, Chonnam National University Hospital, Gwangju; Department of Parasitology2, College of Medicine, Seonam University, Namwon, Korea

Correspondence to: Dong Wook Ryang
Department of Laboratory Medicine, Chonnam National University Medical School, 42 Jebong-ro, Dong-gu, Gwangju 501-757, Korea
Tel: +82-62-220-5340
Fax: +82-62-224-2518
E-mail: dwryang@chonnam.ac.kr

Received: November 24, 2014; Revised: December 24, 2014; Accepted: April 1, 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

ABSTRACT

Conventional formalin-ether concentration method is a gold standard for the diagnosis of parasite infection. However, it may be time-consuming and laborious. We aimed to reveal the clinical usefulness of a modified formalin-ether concentration method using the Para Tube (KS Corporation, Korea) compared with the conventional method. A total of 117 fresh, unpreserved fecal samples composed to 90 negative controls and 27 positive controls with ova of Diphyllobothrium latum/D. nihonkaiense, ova of Clonorchis sinensis and cysts of Giardia lamblia were used in this study. Both methods showed comparable correct identification rate (87.2% for the Para Tube vs. 86.3% for the conventional method).When five samples were examined at once, the Para Tube method reduced the procedure time compared with the conventional method (19 min 58 sec vs. 23 min 18 sec, P=0.0286). We concluded that the modified formalin-ether concentration method using the Para Tube is a rapid, simple, and reliable fecal concentration method for clinical use.

Keywords: Para Tube, Formalin-ether concentration, Parasite, Diagnosis