Transcriptome Analysis of Single Cells (original) (raw)
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07:27 min
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April 25th, 2011
DOI :
April 25th, 2011
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29,450 Views
Jacqueline Morris*1, Jennifer M. Singh*1, James H. Eberwine1,2
1Department of Pharmacology, University of Pennsylvania, 2The Penn Genome Frontiers Institute, University of Pennsylvania
0:05
Title
1:11
Preparing Culture and Pipettes
1:53
Harvesting Cells
5:04
Saving the Cell
6:07
Representative Results for Single Cell Harvest and aRNA Analysis
7:08
Conclusion
In this article we describe a simple method for the harvesting of single cells from rat primary neuronal cultures and subsequent transcriptome analysis using aRNA amplification. This approach is generalizable to any cell type.
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