Mouse Eye Enucleation for Remote High-throughput Phenotyping (original) (raw)

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JournalMedicineMouse eye enucleation for remote high-throughput phenotyping

20.0K Views

05:30 min

November 19th, 2011

DOI :

10.3791/3184-v

November 19th, 2011

20,026 Views


Vinit B. Mahajan1,2, Jessica M. Skeie1,2, Amir H. Assefnia2,3, MaryAnn Mahajan1,2, Stephen H. Tsang2,4

1Department of Ophthalmology and Visual Sciences, University of Iowa, 2Omics Laboratory, University of Iowa, 3School of Dentistry, UCLA, 4Bernard and Shirlee Brown Glaucoma Laboratory, Department of Ophthalmology, College of Physicians and Surgeons, Columbia University

0:05

Title

1:20

Blunt Enucleation

2:27

Posterior Limbal Puncture

3:00

Transfer to Fixative

3:14

Sharp Dissection

4:38

Transport and Shipping

The dissection technique illustrates enucleation of the mouse eye for tissue fixation to perform phenotyping in high-throughput screens.

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