Quantitative Measurement of Invadopodia-mediated Extracellular Matrix Proteolysis in Single and Multicellular Contexts (original) (raw)

JournalBiologyQuantitative measurement of invadopodia-mediated extracellular matrix proteolysis in single and multicellular contexts

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14:23 min

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August 27th, 2012

1:21

Production of Oregon Green 488-gelatin Coated Coverslips

4:48

Plating Cells on Oregon green 488-gelatin Coated Coverslips

5:33

Quantification of Fluorescent Gelatin Degradation by Measuring Normalized Matrix Degradation

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Results: Invadopodia-mediated ECM Proteolysis

We describe the prototypical method for producing microscope coverslips coated with fluorescent gelatin for visualizing invadopodia-mediated matrix degradation. Computational techniques using available software are presented for quantifying the resultant levels of matrix proteolysis by single cells within a mixed population and for multicellular groups encompassing entire microscopic fields.