Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis (original) (raw)

JournalBiologyEndothelial cell tube formation assay for the in vitro study of angiogenesis

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08:12 min

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September 1st, 2014

Katie L. DeCicco-Skinner1, Gervaise H. Henry1, Christophe Cataisson2, Tracy Tabib1, J. Curtis Gwilliam1, Nicholas J. Watson1, Erica M. Bullwinkle1, Lauren Falkenburg1, Rebecca C. O'Neill1, Adam Morin1, Jonathan S. Wiest2

1:29

Growing and Starving Endothelial Cells

2:37

Preparing Endothelial Cells

5:59

Results: Endothelial Tube Formation

The tube formation assay is a fast, quantifiable method for measuring in vitro angiogenesis. Endothelial cells are combined with conditioned media and plated on basement membrane extract. Tube formation occurs within hours and newly formed tubules easily quantified.