Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness (original) (raw)

Authors

DOI:

https://doi.org/10.3855/jidc.7573

Keywords:

Coxsackievirus, Indonesia, febrile illness

Abstract

Introduction: Coxsackievirus B3 (CVB3) virus has been implicated as the causative agent of various outbreaks of clinical disease, including hand, foot, and mouth diseases, aseptic meningitis, acute myocarditis, and inflammatory cardiomyopathy.

Methodology: Two hundred and nine undiagnosed cryopreserved specimens obtained from factory workers in Bandung, Indonesia, who displayed symptoms of acute febrile illness were gathered. Total RNA was isolated from serum and tested by conventional polymerase chain reaction (PCR) using E nterovirus genus-level primers and confirmed by sequencing. Concurrently, the virus was isolated in LLC-MK2 cells.

Results: CVB3 virus was identified in an archived specimen from a patient who presented with symptoms of fever, headache, myalgia, and nausea. Sequencing results of the VP1 region from both the clinical sample and tissue culture supernatant showed 97% homology to a CVB3 virus isolate from Taiwan. Virus propagation in LLC-MK2 cell culture exhibited severe cytopathic effects two days post-inoculation.

Conclusions: We report the first case of CVB3 from an undifferentiated febrile illness specimen from Indonesia.

Author Biographies

Ageng Wiyatno, Eijkman Institute for Molecular Biology, Jakarta, Indonesia

Ungke Antonjaya, Eijkman-Oxford Clinical Research Unit, Jakarta, Indonesia

Silvita Fitri Riswari, Universitas Padjadjaran, Bandung, Indonesia

Bachti Alisjahbana, Universitas Padjadjaran, Bandung, Indonesia

How to Cite

Wiyatno A, Antonjaya U, Ma’roef CN, Riswari SF, Djauhari H, Artika IM, Monagin C, Schneider BS, Myint KS, Alisjahbana B, Safari D, Kosasih H (2016) Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness. J Infect Dev Ctries 10:880–883. doi: 10.3855/jidc.7573

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Brief Original Articles

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