Spandidos Publications: Molecular Medicine Reports (original) (raw)

Print ISSN: 1791-2997 Online ISSN: 1791-3004

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Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells

• Authors:
  • Yi Fan
  • Xiuli Zhang
  • Lina Yang
  • Jun Wang
  • Ye Hu
  • Aishu Bian
  • Jin Liu
  • Jianfei Ma
• View Affiliations / Copyright
  Affiliations: Department of Nephrology, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China
• Pages: 5195-5202
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  Published online on: August 11, 2017
  https://doi.org/10.3892/mmr.2017.7236
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Abstract

Zinc (Zn) deficiency is important for inducing nucleotide-binding domain and leucine‑rich repeat‑containing family, pyrin domain-containing-3 (NLRP3) inflammasome activation in macrophages. However, its function in the NLRP3 inflammasome activation of peritoneal mesothelial cells (PMCs) remains to be elucidated. In the present study, the human PMC (HPMC) line HMrSV5 was co‑treated with high glucose and either ZnSO4 or a Zn chelator. The activity of the NLRP3/caspase‑1 inflammasome was assessed via western blot analysis, immunofluorescence, reverse transcription‑quantitative polymerase chain reaction and ELISA. In addition, the activity of the nuclear factor erythroid 2‑related factor 2 (Nrf2) pathway was detected using western blotting, and the level of reactive oxygen species (ROS) was assessed by 2,7‑dichlorofluorescein fluorescence and flow cytometry. It was found that Zn supplementation inhibited HG‑induced NLRP3 inflammasome activation in the HPMCs by attenuating ROS production. Further experiments revealed that Zn supplementation inhibited the HG‑induced production of ROS through activation of the Nrf2 antioxidant pathway. These results indicated that Zn inhibited NLRP3 inflammasome activation in the HG‑treated HPMCs by activating the Nrf2 antioxidant pathway and reducing the production of ROS.

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Spandidos Publications style

Fan Y, Zhang X, Yang L, Wang J, Hu Y, Bian A, Liu J and Ma J: Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells. Mol Med Rep 16: 5195-5202, 2017.

APA

Fan, Y., Zhang, X., Yang, L., Wang, J., Hu, Y., Bian, A. ... Ma, J. (2017). Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells. Molecular Medicine Reports, 16, 5195-5202. https://doi.org/10.3892/mmr.2017.7236

MLA

Fan, Y., Zhang, X., Yang, L., Wang, J., Hu, Y., Bian, A., Liu, J., Ma, J."Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells". Molecular Medicine Reports 16.4 (2017): 5195-5202.

Chicago

Fan, Y., Zhang, X., Yang, L., Wang, J., Hu, Y., Bian, A., Liu, J., Ma, J."Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells". Molecular Medicine Reports 16, no. 4 (2017): 5195-5202. https://doi.org/10.3892/mmr.2017.7236