Serodiagnosis of Human Toxocariasis Using Adult Somatic and Excretory-Secretory Antigens of Toxocara canis (original) (raw)

Research Article

Department of Zoonotic Diseases, National Research Center, Post Box 12622, El-Tahrir Street, Dokki, Giza, Egypt

Mona S. Mahmoud
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Center, Post Box 12622, El-Tahrir Street, Dokki, Giza, Egypt

Human toxocariasis is one of the most common zoonotic helminthiases. Serological diagnosis remains the main tool for the diagnosis of toxocariasis. Serum samples collected from 57 confirmed cases of human toxocariasis and 26 healthy subjects (negative controls) were tested for anti-Toxocara antibodies by the enzyme-linked immunosorbent assay (ELISA) and enzyme linked immunotransfer blot (EITB) using adult Toxocara canis antigens; somatic antigen (TcSA) and its purified fractions (P-F1, P-F2 and P-F3) and excretory-secretory antigen (TcESA) to evaluate their sensitivity and specificity for the diagnosis of human toxocariasis. ELISA results showed that TcESA is the antigen of choice for diagnosis of human toxocariasis as it showed the highest specificity and sensitivity (100 and 92.30%, respectively). The immunoblot profile of TcESA, TcSA, P-F1, P-F2 and P-F3 reacted with rabbit hyperimmune serum prepared against TcSA showed common immunoreactive bands at 88.00, 85.00, 80.00, 47.462, 40.687, 35.706, 30.527, 21.983 and 19.285 kDa. The immunoblot patterns of them reacted with positive human sera displayed common bands at 47.462, 40.687, 21.983 and 19.285 kDa. The immunoblot profile of TcESA reacted with positive human (58.739, 47.462, 40.687, 30.527, 29.190, 21.983 and 19.285 kDa) and rabbit hyperimmune (94.00, 88.00, 85.00, 80.00, 47.462, 40.687, 35.706, 30.527, 21.983 and 19.285 kDa) sera showed common bands at 47.462, 40.687, 30.527, 21.983 and 19.285 kDa. So, we may suggest that 58.739 and 29.190 kDa polypeptides of the adult T. canis secretory-excretory antigen are specific for Toxocara infection in human and merit further evaluation as candidates for use in the diagnosis of human toxocariasis.

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How to cite this article

Nawal A. Hassanain and Mona S. Mahmoud, 2008. Serodiagnosis of Human Toxocariasis Using Adult Somatic and Excretory-Secretory Antigens of Toxocara canis. Research Journal of Parasitology, 3: 85-91.

URL: https://scialert.net/abstract/?doi=jp.2008.85.91

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