Granada Virus: a Natural Phlebovirus Reassortant of the Sandfly Fever Naples Serocomplex with Low Seroprevalence in Humans (original) (raw)

Print ISSN:

0002-9637

Online ISSN:

1476-1645

• Introduction
• Materials and Methods
  • Sandfly collection, nucleic acids extraction, and isolation of viruses in cell culture.
  • Molecular identification of phleboviruses.
  • Genomic sequencing.
  • Phylogenetic analysis of complete sequences.
  • Serological assays.
• Results
  • Identification of GRV in sandflies.
  • Phylogenetic analysis of the complete sequence of Granada virus.
  • Detection of antibodies against GRV.
• Discussion

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    NicholSTBeatyBJElliottRMGoldbachRPlyusinASchmaljohnCTeshR, 2005. Family Bunyaviridae. FauquetCMMayoMAManiloffJDesselbergerUBallLA, eds. Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. San Diego, CA: Elsevier Academic Press, 695–723.
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    DionisioDValassinaMCiufoliniMGVivarelliAEspertiFCusiMGMarchiAMazzoliFLuppiC, 2001. Encephalitis without meningitis due to sandfly fever virus serotype toscana. Clin Infect Dis 32: 1241–1243.
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  • Export Citation
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    BraitoACiufoliniMGPippiLCorbisieroRFiorentiniCGistriAToscanoL, 1998. Phlebotomus-transmitted toscana virus infections of the central nervous system: a seven-year experience in Tuscany. Scand J Infect Dis 30: 505–508.
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  • Export Citation
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  • PubMed
    Mendoza-MonteroJGamez-RuedaMINavarro-MariJMde la Rosa-FraileMOyonarte-GomezS, 1998. Infections due to sandfly fever virus serotype toscana in Spain. Clin Infect Dis 27: 434–436.
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  • Export Citation
• 1. Nicoletti L, Verani P, Caciolli S, Ciufolini MG, Renzi A, Bartolozzi D, Paci P, Leoncini F, Padovani P, Traini E, Baldereschi M, Balducci M, 1991. Central nervous system involvement during infection by phlebovirus toscana of residents in natural foci in central Italy (1977–1988). Am J Trop Med Hyg 45: 429–434.
  • PubMed
    NicolettiLVeraniPCaciolliSCiufoliniMGRenziABartolozziDPaciPLeonciniFPadovaniPTrainiEBaldereschiMBalducciM, 1991. Central nervous system involvement during infection by phlebovirus toscana of residents in natural foci in central Italy (1977–1988). Am J Trop Med Hyg 45: 429–434.
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    CharrelRNMoureauGTemmamSIzriAMartyPParolaPda RosaATTeshRBLamballerieX, 2009. Massilia virus, a novel phlebovirus (Bunyaviridae) isolated from sandflies in the Mediterranean. Vector Borne Zoonotic Dis 9: 519–530.
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• Abstract/Excerpt
• Author Notes

A new member of the phlebovirus genus, tentatively named Granada virus, was detected in sandflies collected in Spain. By showing the presence of specific neutralizing antibodies in human serum collected in Granada, we show that Granada virus infects humans. The analysis of the complete genome of Granada virus revealed that this agent is likely to be a natural reassortant of the recently described Massilia virus (donor of the long and short segments) with a yet unidentified phlebovirus (donor of the medium segment).

Author Notes

*Address correspondence to Maria Paz Sánchez-Seco, Laboratory of Arbovirus and Imported Viral Diseases, National Center of Microbiology, Institute of Health “Carlos III,” Ctra Pozuelo-Majadahonda km 2, 28220 Madrid, Spain. E-mail: paz.sanchez@isciii.es

†These authors contributed equally.

Authors' addresses: Ximena Collao, Antonio Tenorio, and María Paz Sánchez-Seco, Laboratory of Arbovirus and Imported Viral Diseases, National Center of Microbiology, Institute of Health “Carlos III,” Madrid, Spain, E-mails: ximecf1@hotmail.com, atenorio@isciii.es, and paz.sanchez@isciii.es. Gustavo Palacios and W. Ian Lipkin, Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, New York, E-mails: gp2050@columbia.edu and wil2001@columbia.edu. Fernando de Ory, Serology Section, National Center of Microbiology, Institute of Health “Carlos III,” Madrid, Spain, E-mail: fory@isciii.es. Sara Sanbonmatsu, Mercedes Pérez-Ruiz, and José María Navarro, Service of Microbiology, Universitary Hospital “Virgen de las Nieve,” Granada, Spain, E-mails: sbmsara@yahoo.es, mercedes.perez.ruiz.sspa@juntadeandalucia.es, and josem.navarro.sspa@juntadeandalucia.es. Ricardo Molina, Service of Parasitology, National Center of Microbiology, Institute of Health “Carlos III,” Madrid, Spain, E-mail: rmolina@isciii.es. Stephen K. Hutchison, 454 Life Sciences, Branford, CT, E-mail: stephen.hutchison@roche.com. Ximena Collao actual address: Virology, Medicine School, Universidad de Valparaíso, Valparaiso, Chile.

Copyright:

©The American Society of Tropical Medicine and Hygiene 2010

Received:

23 Nov 2009

|

Accepted:

18 May 2010

|

Published Online:

05 Oct 2010

Print ISSN:

0002-9637

Online ISSN:

1476-1645

• Introduction
• Materials and Methods
  • Sandfly collection, nucleic acids extraction, and isolation of viruses in cell culture.
  • Molecular identification of phleboviruses.
  • Genomic sequencing.
  • Phylogenetic analysis of complete sequences.
  • Serological assays.
• Results
  • Identification of GRV in sandflies.
  • Phylogenetic analysis of the complete sequence of Granada virus.
  • Detection of antibodies against GRV.
• Discussion

ProCite

RefWorks

Reference Manager

• 1. Nichol ST, Beaty BJ, Elliott RM, Goldbach R, Plyusin A, Schmaljohn C, Tesh R, 2005. Family Bunyaviridae. Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA, eds. Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. San Diego, CA: Elsevier Academic Press, 695–723.
  • PubMed
    NicholSTBeatyBJElliottRMGoldbachRPlyusinASchmaljohnCTeshR, 2005. Family Bunyaviridae. FauquetCMMayoMAManiloffJDesselbergerUBallLA, eds. Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. San Diego, CA: Elsevier Academic Press, 695–723.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Gould EA, Higgs S, 2009. Impact of climate change and other factors on emerging arbovirus diseases. Trans R Soc Trop Med Hyg 103: 109–121.
  • PubMed
    GouldEAHiggsS, 2009. Impact of climate change and other factors on emerging arbovirus diseases. Trans R Soc Trop Med Hyg 103: 109–121.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Valassina M, Meacci F, Valensin PE, Cusi MG, 2000. Detection of neurotropic viruses circulating in Tuscany: the incisive role of Toscana virus. J Med Virol 60: 86–90.
  • PubMed
    ValassinaMMeacciFValensinPECusiMG, 2000. Detection of neurotropic viruses circulating in Tuscany: the incisive role of Toscana virus. J Med Virol 60: 86–90.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Nicoletti L, Ciufolini MG, Verani P, 1996. Sandfly fever viruses in Italy. Arch Virol Suppl 11: 41–47.
  • PubMed
    NicolettiLCiufoliniMGVeraniP, 1996. Sandfly fever viruses in Italy. Arch Virol Suppl 11: 41–47.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Dionisio D, Valassina M, Ciufolini MG, Vivarelli A, Esperti F, Cusi MG, Marchi A, Mazzoli F, Luppi C, 2001. Encephalitis without meningitis due to sandfly fever virus serotype toscana. Clin Infect Dis 32: 1241–1243.
  • PubMed
    DionisioDValassinaMCiufoliniMGVivarelliAEspertiFCusiMGMarchiAMazzoliFLuppiC, 2001. Encephalitis without meningitis due to sandfly fever virus serotype toscana. Clin Infect Dis 32: 1241–1243.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Braito A, Ciufolini MG, Pippi L, Corbisiero R, Fiorentini C, Gistri A, Toscano L, 1998. Phlebotomus-transmitted toscana virus infections of the central nervous system: a seven-year experience in Tuscany. Scand J Infect Dis 30: 505–508.
  • PubMed
    BraitoACiufoliniMGPippiLCorbisieroRFiorentiniCGistriAToscanoL, 1998. Phlebotomus-transmitted toscana virus infections of the central nervous system: a seven-year experience in Tuscany. Scand J Infect Dis 30: 505–508.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Braito A, Corbisiero R, Corradini S, Fiorentini C, Ciufolini MG, 1998. Toscana virus infections of the central nervous system in children: a report of 14 cases. J Pediatr 132: 144–148.
  • PubMed
    BraitoACorbisieroRCorradiniSFiorentiniCCiufoliniMG, 1998. Toscana virus infections of the central nervous system in children: a report of 14 cases. J Pediatr 132: 144–148.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Hemmersbach-Miller M, Parola P, Charrel RN, Paul Durand J, Brouqui P, 2004. Sandfly fever due to toscana virus: an emerging infection in southern France. Eur J Intern Med 15: 316–317.
  • PubMed
    Hemmersbach-MillerMParolaPCharrelRNPaul DurandJBrouquiP, 2004. Sandfly fever due to toscana virus: an emerging infection in southern France. Eur J Intern Med 15: 316–317.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Ehrnst A, Peters CJ, Niklasson B, Svedmyr A, Holmgren B, 1985. Neurovirulent toscana virus (a sandfly fever virus) in Swedish man after visit to Portugal. Lancet 1: 1212–1213.
  • PubMed
    EhrnstAPetersCJNiklassonBSvedmyrAHolmgrenB, 1985. Neurovirulent toscana virus (a sandfly fever virus) in Swedish man after visit to Portugal. Lancet 1: 1212–1213.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Mendoza-Montero J, Gamez-Rueda MI, Navarro-Mari JM, de la Rosa-Fraile M, Oyonarte-Gomez S, 1998. Infections due to sandfly fever virus serotype toscana in Spain. Clin Infect Dis 27: 434–436.
  • PubMed
    Mendoza-MonteroJGamez-RuedaMINavarro-MariJMde la Rosa-FraileMOyonarte-GomezS, 1998. Infections due to sandfly fever virus serotype toscana in Spain. Clin Infect Dis 27: 434–436.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Nicoletti L, Verani P, Caciolli S, Ciufolini MG, Renzi A, Bartolozzi D, Paci P, Leoncini F, Padovani P, Traini E, Baldereschi M, Balducci M, 1991. Central nervous system involvement during infection by phlebovirus toscana of residents in natural foci in central Italy (1977–1988). Am J Trop Med Hyg 45: 429–434.
  • PubMed
    NicolettiLVeraniPCaciolliSCiufoliniMGRenziABartolozziDPaciPLeonciniFPadovaniPTrainiEBaldereschiMBalducciM, 1991. Central nervous system involvement during infection by phlebovirus toscana of residents in natural foci in central Italy (1977–1988). Am J Trop Med Hyg 45: 429–434.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Charrel RN, Moureau G, Temmam S, Izri A, Marty P, Parola P, da Rosa AT, Tesh RB, Lamballerie X, 2009. Massilia virus, a novel phlebovirus (Bunyaviridae) isolated from sandflies in the Mediterranean. Vector Borne Zoonotic Dis 9: 519–530.
  • PubMed
    CharrelRNMoureauGTemmamSIzriAMartyPParolaPda RosaATTeshRBLamballerieX, 2009. Massilia virus, a novel phlebovirus (Bunyaviridae) isolated from sandflies in the Mediterranean. Vector Borne Zoonotic Dis 9: 519–530.
    )| false
  • Search Google Scholar
  • Export Citation
• 1. Sanchez-Seco MP, Vazquez A, Collao X, Hernandez L, Aranda C, Ruiz S, Escosa R, Marques E, Bustillo MA, Molero F, Tenorio A, 2010. Surveillance of arboviruses in Spanish wetlands: detection of new flavi- and phleboviruses. Vector Borne Zoonotic Dis 10: 203–206.
  • PubMed
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