Matrix remodel (original) (raw)

figure

posted on 2017-08-04, 22:07 authored by Jihan KimJihan Kim, Jingchen Feng, Christopher A. R. Jones, Xiaoming Mao, Leonard M. Sander, Herbert Levine, Bo Sun

Cell-embedded collagen gels are prepared by diluting
and neutralizing high concentration type-I collagen solu-
tion (10 mg/ml, Corning) with NaOH, cell suspension,
growth medium, and 10X PBS into 1.5 mg/ml. The neu-
tralized solution is immediately placed in a tissue culture
incubator (NuAire) to polymerize at 37 ◦ C for 40 min-7
utes, then the maturation time starts to count.
To release cell traction force, we dilute Cytochalasin-
D (Sigma-Aldrich) with PBS to a 1:1000 ratio and add
directly to the 3D culture samples. We allow 2 hours to
complete the treatment before washing the sample with
growth medium.