Infection of Clinostomum complanatum (Treamdoda: Digenea) metacercaria in cultured southern top mouthed minnow Pseudorasbora parva -Journal of fish pathology (original) (raw)

Abstract

Many metacercariae in the musculature of southern top mouthed minnow (Pseudorasbora parva) cultured at a farm in Chonbuk Province. The excysted metacercariae looked yellowish white and were 4.02pm1.804.02{\pm}1.804.02pm1.80 mm (1.66-5.81) in length and 1.27pm0.271.27{\pm}0.271.27pm0.27 mm (1.01-1.64) in width for acetocarmin-stained specimens. The oral sucker measuring 0.18pm0.050.18{\pm}0.050.18pm0.05 mm (0.11-0.24) in length and 0.23pm0.070.23{\pm}0.070.23pm0.07 mm (0.15-0.33) in width, was located at the anterior end of the body. The ventral sucker situated at the fourth third of the body were measured 0.47pm0.080.47{\pm}0.080.47pm0.08 mm (0.35-0.53) in length and 0.55pm0.090.55{\pm}0.090.55pm0.09 mm (0.43-0.63) in width. The tongue-shaped metacercariae were identified as Clinostomum complanatum on the basis of morphological characteristics. The metacercaria was also confirmed to be C. complanatum using PCR reaction with C. complanatum-specific primer sets, Trem 18S F and Ccom670 R. No histopathological changes in the infected fish were observed except necrosis and exfoliation of epidermis in the skin and atrophy of muscle fibers.

노지 양식장에서 비단잉어와 함께 사육중인 참붕어의 미병부, 체측근, 복부 및 아가미 뚜껑 내부에 황백색의 cyst내의 metacercaria를 탈낭시켜 형태학적 및 분자생물학적으로 조사한 결과 Clinostomum complanatum으로 동정되었다. 병리조직학적으로는 상피세포의 박리와 괴사 및 cyst주변의 근섬유의 위축이외에 다른 조직의 변화는 발견되지 않았다.