CPTC-HLA-DPB1-2 (original) (raw)

Product Form :

Supernatant 1.0 ml

Catalog Fields

Clone ID/Product Name: CPTC-HLA-DPB1-2

Available to For-Profits: Yes

Alternate Antibody Name:

Gene Symbol: HLA-DPB1

Ab Isotype: Rabbit IgG

Gene Name:

RRID:

Entrez Gene ID: 3115

Clonality: Monoclonal

Immunogen: Synthetic peptides

Clone:

Immunogen Sequence: AVTELGRPDEEYWNSQK AVTELGRPDEDYWNSQK

Myeloma Strain: 240E-W2

Epitope Mapped: No

Antigen Name: Major Histocompatibility Complex, Class II, DP Beta 1 Peptide 2

Epitope Location or Sequence:

Alternate Antigen Name:

Deposit Date: 3/3/2021

Antigen Molecular Weight: 29.1 kDa

Depositor: Paulovich, A.P.

Antigen Sequence:

Depositor Institution: Fred Hutchinson Cancer Research Center

Antigen Species: Human

Depositor Notes: This receptor is fundamental to the presentation of antigen in adaptive immunity.

Host Species: rabbit

Hybridoma Cells Available (Non-Profit): No

Confirmed Species Reactivity: Human

Additional Information:

Predicted Species Reactivity:

Human Protein Atlas:

Recommended Applications:

All cell products contain the antimicrobial ProClin. Click here for additional information.

These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.

For your Materials & Methods section:

CPTC-HLA-DPB1-2 was deposited to the DSHB by Paulovich, A.P. (DSHB Hybridoma Product CPTC-HLA-DPB1-2)

Storage and Handling Recommendations

Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

Usage Recommendations

The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).