bhuwaneshwar S mishra | University of Delhi (original) (raw)
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Papers by bhuwaneshwar S mishra
Sensory Biology of Plants, 2019
The survival of organisms is dependent on the perception of various external and internal cues an... more The survival of organisms is dependent on the perception of various external and internal cues and modulating growth according to the available conditions. This is achieved through highly coordinated and interconnected signalling pathways which are highly complex in eukaryotic systems. In order to circumvent the sessile nature, plants are evolved to have enhanced plasticity and robust environmental sensing mechanisms. Sugars produced by the plants are perceived by a dedicated set of receptors which leads to the modulation of the specific signalling pathway to ultimately fine-tune plant growth and defence responses according to the sugar and energy availability. Different phytohormone signalling pathways which originated at different facets of plant evolution play a pivotal role in controlling the growth, development and defence strategies. Research in the past two decades uncovered the extent of interaction of sugar and phytohormone signalling pathways in controlling and fine-tuning various plant growth and stress responses. The following chapter concisely summarizes the molecular and physiological interaction of different sugar signalling pathways with hormone signalling pathways which is ultimately important in the regulation of plant development and stress responses.
Physiologia Plantarum, 2021
The divergence of natural stress tolerance mechanisms between species is an intriguing phenomenon... more The divergence of natural stress tolerance mechanisms between species is an intriguing phenomenon. To study it in rice, a comparative transcriptome analysis was carried out in 'heading' stage tissue (flag leaf, panicles and roots) of Nagina 22 (N22; drought-tolerant) and IR64 (drought-sensitive) plants subjected to field drought. Interestingly, N22 showed almost double the number of differentially expressed genes (DEGs) than IR64. Many DEGs colocalized within drought-related QTLs responsible for grain yield and drought tolerance and also associated with drought tolerance and critical drought-related plant traits such as leaf rolling, trehalose content, sucrose and cellulose content. Besides, co-expression analysis of the DEGs revealed several 'hub' genes known to actively regulate drought stress response. Strikingly, 1366 DEGs, including 21 'hub' genes, showed a distinct opposite regulation in the two rice varieties under similar drought conditions. Annotation of these variety-specific DEGs (VS-DEGs) revealed that they are distributed in various biological pathways. Furthermore, 103 VS-DEGs were found to physically interact with over 1300 genes, including 32 that physically interact with other VS-DEGs as well. The promoter region of these genes have sequence variations among the two rice varieties, which might be in part responsible for their unique expression pattern. This article is protected by copyright. All rights reserved.
Frontiers in Plant Science, Oct 25, 2017
BIG GRAIN1 (BG1) is an auxin-regulated gene which functions in auxin pathway and positively regul... more BIG GRAIN1 (BG1) is an auxin-regulated gene which functions in auxin pathway and positively regulates biomass, grain size and yield in rice. However, the evolutionary origin and divergence of these genes are still unknown. In this study, we found that BG genes are probably originated in seed plants. We also identified that seed plants evolved a class of BIG GRAIN LIKE (BGL) genes which share conserved middle and C-terminal motifs with BG. The BG genes were present in all monocot and eudicot species analyzed; however, the BGL genes were absent in few monocot lineages. Both BG and BGL were found to be serine-rich proteins; however, differences in expansion and rates of retention after whole genome duplication events were observed. Promoters of BG and BGL genes were found to be enriched with auxin-responsive elements and the Arabidopsis thaliana BG and BGL genes were found to be auxin-regulated. The auxin-induced expression of AthBG2 was found to be dependent on the conserved ARF17/19 module. Proteinprotein interaction analysis identified that AthBG2 interact with regulators of splicing, transcription and chromatin remodeling. Taken together, this study provides interesting insights about BG and BGL genes and incentivizes future work in this gene family which has the potential to be used for crop manipulation.
Physiologia Plantarum, 2021
Under the natural environment, nutrient signals interact with phytohormones to coordinate and rep... more Under the natural environment, nutrient signals interact with phytohormones to coordinate and reprogram plant growth and survival. Sugars are important molecules that control almost all morphological and physiological processes in plants, ranging from seed germination to senescence. In addition to their functions as energy resources, osmoregulation, storage molecules, and structural components, sugars function as signaling molecules and interact with various plant signaling pathways, such as hormones, stress and light to modulate growth and development according to fluctuating environmental conditions. Auxin, being an important phytohormone, is associated with almost all stages of the plant's life cycle and also plays a vital role in response to the dynamic environment for better growth and survival. In the previous years, substantial progress has been made that showed a range of common responses mediated by sugars and auxin signaling. This review discusses how sugar signaling affects auxin at various levels from its biosynthesis to perception and downstream gene activation. On the same note, the review also highlights the role of auxin signaling in fine-tuning sugar metabolism and carbon partitioning. Furthermore, we discussed the crosstalk between the two signaling machineries in the regulation of various biological processes, such as gene expression, cell cycle, development, root system architecture and shoot growth. In conclusion, the review emphasized the role of sugar and auxin crosstalk in the regulation of several agriculturally important traits. Thus, engineering of sugar and auxin signaling pathways could potentially provide new avenues to manipulate for agricultural purposes. This article is protected by copyright. All rights reserved.
Plant Molecular Biology Reporter, 1989
p lan t genes are usually designated byacronyms or by trivial names. The former are useful becaus... more p lan t genes are usually designated byacronyms or by trivial names. The former are useful because of their high information content, the latter because they were rooted in taxonomy. As the field expands, this free systematicsiscausing confusion: genesaregivennonspecific designations that are unrelated to names assigned to homologous genes in other species. This tendency, together with our expanding ability to probe biological systems by isolating genes and proteins of unknown function, could lead to more confusion if we are not careful. This brief review is intended to illustrate problems encountered in naming genes and proteins. Two examples are first recounted to familiarize the reader with difficulties that can arise when naming genes on the basis of known functions of encoded proteins or on sequence homologies. The third example describes the names given to genes which are responsive to the plant hormone abscisic acid (ABA). These genes are widely studied because ABA may mediate the onset or maintenance of seed dormancy and also the response of plants to osmotic and wound-Abbreviations. ABA, abscisic acid; LEA, late embryogenesis abundant; PAPI, putative amylase/protease inhibitor; PLTP, phospholipid transfer protein.
Polar Auxin Transport, 2013
![Research paper thumbnail of {"__content__"=>"Genome-Wide Identification and Expression, Protein-Protein Interaction and Evolutionary Analysis of the Seed Plant-Specific and Gene Family.", "i"=>[{"__content__"=>"BIG GRAIN"}, {"__content__"=>"BIG GRAIN LIKE"}]}](https://attachments.academia-assets.com/70924294/thumbnails/1.jpg)
](Frontiers in plant science, 2017
BIG GRAIN1 (BG1) is an auxin-regulated gene which functions in auxin pathway and positively regul... more BIG GRAIN1 (BG1) is an auxin-regulated gene which functions in auxin pathway and positively regulates biomass, grain size and yield in rice. However, the evolutionary origin and divergence of these genes are still unknown. In this study, we found that BG genes are probably originated in seed plants. We also identified that seed plants evolved a class of BIG GRAIN LIKE (BGL) genes which share conserved middle and C-terminal motifs with BG. The BG genes were present in all monocot and eudicot species analyzed; however, the BGL genes were absent in few monocot lineages. Both BG and BGL were found to be serine-rich proteins; however, differences in expansion and rates of retention after whole genome duplication events were observed. Promoters of BG and BGL genes were found to be enriched with auxin-responsive elements and the Arabidopsis thaliana BG and BGL genes were found to be auxin-regulated. The auxin-induced expression of AthBG2 was found to be dependent on the conserved ARF17/19 module. Proteinprotein interaction analysis identified that AthBG2 interact with regulators of splicing, transcription and chromatin remodeling. Taken together, this study provides interesting insights about BG and BGL genes and incentivizes future work in this gene family which has the potential to be used for crop manipulation.
Frontiers in Plant Science
Plant Signaling & Behavior, 2009
P lants have the ability to adjust its physiology and metabolism to the changes of nutrient avail... more P lants have the ability to adjust its physiology and metabolism to the changes of nutrient availability in the environment. Since a number of common responses are regulated by sugar and auxin, the obvious question arises is whether sugar and auxin act interdependently to bring about changes in plant morphology. In the February issue of the PLoS ONE, 1 we presented detailed investigation of glucose and auxin signaling interaction in controlling root growth and development in Arabidopsis thaliana seedlings. Further analysis of tissue specific regulation of glucose auxin signaling interaction may provide some insight as to how these two signaling molecules interact to control the morphogenic changes during seedling development.
PLOS One, 2009
Background: Plant root growth and development is highly plastic and can adapt to many environment... more Background: Plant root growth and development is highly plastic and can adapt to many environmental conditions. Sugar signaling has been shown to affect root growth and development by interacting with phytohormones such as gibberellins, cytokinin and abscisic acid. Auxin signaling and transport has been earlier shown to be controlling plant root length, number of lateral roots, root hair and root growth direction.
p lan t genes are usually designated byacronyms or by trivial names.
Sensory Biology of Plants, 2019
The survival of organisms is dependent on the perception of various external and internal cues an... more The survival of organisms is dependent on the perception of various external and internal cues and modulating growth according to the available conditions. This is achieved through highly coordinated and interconnected signalling pathways which are highly complex in eukaryotic systems. In order to circumvent the sessile nature, plants are evolved to have enhanced plasticity and robust environmental sensing mechanisms. Sugars produced by the plants are perceived by a dedicated set of receptors which leads to the modulation of the specific signalling pathway to ultimately fine-tune plant growth and defence responses according to the sugar and energy availability. Different phytohormone signalling pathways which originated at different facets of plant evolution play a pivotal role in controlling the growth, development and defence strategies. Research in the past two decades uncovered the extent of interaction of sugar and phytohormone signalling pathways in controlling and fine-tuning various plant growth and stress responses. The following chapter concisely summarizes the molecular and physiological interaction of different sugar signalling pathways with hormone signalling pathways which is ultimately important in the regulation of plant development and stress responses.
Physiologia Plantarum, 2021
The divergence of natural stress tolerance mechanisms between species is an intriguing phenomenon... more The divergence of natural stress tolerance mechanisms between species is an intriguing phenomenon. To study it in rice, a comparative transcriptome analysis was carried out in 'heading' stage tissue (flag leaf, panicles and roots) of Nagina 22 (N22; drought-tolerant) and IR64 (drought-sensitive) plants subjected to field drought. Interestingly, N22 showed almost double the number of differentially expressed genes (DEGs) than IR64. Many DEGs colocalized within drought-related QTLs responsible for grain yield and drought tolerance and also associated with drought tolerance and critical drought-related plant traits such as leaf rolling, trehalose content, sucrose and cellulose content. Besides, co-expression analysis of the DEGs revealed several 'hub' genes known to actively regulate drought stress response. Strikingly, 1366 DEGs, including 21 'hub' genes, showed a distinct opposite regulation in the two rice varieties under similar drought conditions. Annotation of these variety-specific DEGs (VS-DEGs) revealed that they are distributed in various biological pathways. Furthermore, 103 VS-DEGs were found to physically interact with over 1300 genes, including 32 that physically interact with other VS-DEGs as well. The promoter region of these genes have sequence variations among the two rice varieties, which might be in part responsible for their unique expression pattern. This article is protected by copyright. All rights reserved.
Frontiers in Plant Science, Oct 25, 2017
BIG GRAIN1 (BG1) is an auxin-regulated gene which functions in auxin pathway and positively regul... more BIG GRAIN1 (BG1) is an auxin-regulated gene which functions in auxin pathway and positively regulates biomass, grain size and yield in rice. However, the evolutionary origin and divergence of these genes are still unknown. In this study, we found that BG genes are probably originated in seed plants. We also identified that seed plants evolved a class of BIG GRAIN LIKE (BGL) genes which share conserved middle and C-terminal motifs with BG. The BG genes were present in all monocot and eudicot species analyzed; however, the BGL genes were absent in few monocot lineages. Both BG and BGL were found to be serine-rich proteins; however, differences in expansion and rates of retention after whole genome duplication events were observed. Promoters of BG and BGL genes were found to be enriched with auxin-responsive elements and the Arabidopsis thaliana BG and BGL genes were found to be auxin-regulated. The auxin-induced expression of AthBG2 was found to be dependent on the conserved ARF17/19 module. Proteinprotein interaction analysis identified that AthBG2 interact with regulators of splicing, transcription and chromatin remodeling. Taken together, this study provides interesting insights about BG and BGL genes and incentivizes future work in this gene family which has the potential to be used for crop manipulation.
Physiologia Plantarum, 2021
Under the natural environment, nutrient signals interact with phytohormones to coordinate and rep... more Under the natural environment, nutrient signals interact with phytohormones to coordinate and reprogram plant growth and survival. Sugars are important molecules that control almost all morphological and physiological processes in plants, ranging from seed germination to senescence. In addition to their functions as energy resources, osmoregulation, storage molecules, and structural components, sugars function as signaling molecules and interact with various plant signaling pathways, such as hormones, stress and light to modulate growth and development according to fluctuating environmental conditions. Auxin, being an important phytohormone, is associated with almost all stages of the plant's life cycle and also plays a vital role in response to the dynamic environment for better growth and survival. In the previous years, substantial progress has been made that showed a range of common responses mediated by sugars and auxin signaling. This review discusses how sugar signaling affects auxin at various levels from its biosynthesis to perception and downstream gene activation. On the same note, the review also highlights the role of auxin signaling in fine-tuning sugar metabolism and carbon partitioning. Furthermore, we discussed the crosstalk between the two signaling machineries in the regulation of various biological processes, such as gene expression, cell cycle, development, root system architecture and shoot growth. In conclusion, the review emphasized the role of sugar and auxin crosstalk in the regulation of several agriculturally important traits. Thus, engineering of sugar and auxin signaling pathways could potentially provide new avenues to manipulate for agricultural purposes. This article is protected by copyright. All rights reserved.
Plant Molecular Biology Reporter, 1989
p lan t genes are usually designated byacronyms or by trivial names. The former are useful becaus... more p lan t genes are usually designated byacronyms or by trivial names. The former are useful because of their high information content, the latter because they were rooted in taxonomy. As the field expands, this free systematicsiscausing confusion: genesaregivennonspecific designations that are unrelated to names assigned to homologous genes in other species. This tendency, together with our expanding ability to probe biological systems by isolating genes and proteins of unknown function, could lead to more confusion if we are not careful. This brief review is intended to illustrate problems encountered in naming genes and proteins. Two examples are first recounted to familiarize the reader with difficulties that can arise when naming genes on the basis of known functions of encoded proteins or on sequence homologies. The third example describes the names given to genes which are responsive to the plant hormone abscisic acid (ABA). These genes are widely studied because ABA may mediate the onset or maintenance of seed dormancy and also the response of plants to osmotic and wound-Abbreviations. ABA, abscisic acid; LEA, late embryogenesis abundant; PAPI, putative amylase/protease inhibitor; PLTP, phospholipid transfer protein.
Polar Auxin Transport, 2013
Frontiers in plant science, 2017
BIG GRAIN1 (BG1) is an auxin-regulated gene which functions in auxin pathway and positively regul... more BIG GRAIN1 (BG1) is an auxin-regulated gene which functions in auxin pathway and positively regulates biomass, grain size and yield in rice. However, the evolutionary origin and divergence of these genes are still unknown. In this study, we found that BG genes are probably originated in seed plants. We also identified that seed plants evolved a class of BIG GRAIN LIKE (BGL) genes which share conserved middle and C-terminal motifs with BG. The BG genes were present in all monocot and eudicot species analyzed; however, the BGL genes were absent in few monocot lineages. Both BG and BGL were found to be serine-rich proteins; however, differences in expansion and rates of retention after whole genome duplication events were observed. Promoters of BG and BGL genes were found to be enriched with auxin-responsive elements and the Arabidopsis thaliana BG and BGL genes were found to be auxin-regulated. The auxin-induced expression of AthBG2 was found to be dependent on the conserved ARF17/19 module. Proteinprotein interaction analysis identified that AthBG2 interact with regulators of splicing, transcription and chromatin remodeling. Taken together, this study provides interesting insights about BG and BGL genes and incentivizes future work in this gene family which has the potential to be used for crop manipulation.
Frontiers in Plant Science
Plant Signaling & Behavior, 2009
P lants have the ability to adjust its physiology and metabolism to the changes of nutrient avail... more P lants have the ability to adjust its physiology and metabolism to the changes of nutrient availability in the environment. Since a number of common responses are regulated by sugar and auxin, the obvious question arises is whether sugar and auxin act interdependently to bring about changes in plant morphology. In the February issue of the PLoS ONE, 1 we presented detailed investigation of glucose and auxin signaling interaction in controlling root growth and development in Arabidopsis thaliana seedlings. Further analysis of tissue specific regulation of glucose auxin signaling interaction may provide some insight as to how these two signaling molecules interact to control the morphogenic changes during seedling development.
PLOS One, 2009
Background: Plant root growth and development is highly plastic and can adapt to many environment... more Background: Plant root growth and development is highly plastic and can adapt to many environmental conditions. Sugar signaling has been shown to affect root growth and development by interacting with phytohormones such as gibberellins, cytokinin and abscisic acid. Auxin signaling and transport has been earlier shown to be controlling plant root length, number of lateral roots, root hair and root growth direction.
p lan t genes are usually designated byacronyms or by trivial names.