Yongliang Fan | Duke University (original) (raw)
Papers by Yongliang Fan
Metabolites, 2021
Benzoxazinoids are important secondary metabolites in gramineae plants and have inhibitory and to... more Benzoxazinoids are important secondary metabolites in gramineae plants and have inhibitory and toxic effects against a wide range of herbivore pests. However, the relationship between benzoxazinoid level and plant resistance to aphids remains controversial. In this study, we investigated the relationship between benzoxazinoids composition and concentration in wheat leaves and the resistance to the grain aphid Sitobion avenae. Overall, six benzoxazinoids were detected and identified by mass spectrometry based metabolites profiling, including three lactams, two hydroxamic acids, and one methyl derivative. The constitutive levels of these benzoxazinoids were significantly different among the wheat varieties/lines. However, none of these benzoxazinoids exhibited considerable correlation with aphid resistance. S. avenae feeding elevated the level of 2-O-β-D-glucopyranosyloxy-4,7-dimethoxy-(2H)-1,4-benzoxazin-3(4H)-one (HDMBOA-Glc) and reduced the level of 2-O-β-D-glucopyranosyloxy-4-hydr...
Scientific reports, Jan 20, 2016
Low proportion of essential amino acids (EAAs) is one of the barriers for animals to use phloem a... more Low proportion of essential amino acids (EAAs) is one of the barriers for animals to use phloem as a diet. Endosymbionts with EAAs synthesis functions are considered crucial for ameliorating the lack of EAAs in insects' diets. In this study, we transferred the insects from a cabbage-reared Myzus persicae population onto 3 new plant species including eggplant, tobacco and spinach. The performance on these plants was evaluated and the dynamics of endosymbionts in relation to this host alternation were recorded. We found that the EAAs ratio in phloem was largely determined by the concentrations of non-essential amino acids and the higher proportion of EAAs seemed to favor the population establishment on new plant species and the growth of primary endosymbionts inside insects, which indicated that nitrogen quality was an important factor for aphids to infest and spread on new plant hosts.
International Journal of Molecular Sciences, 2021
Insect cuticular hydrocarbons (CHCs) are organic compounds of the surface lipid layer, which func... more Insect cuticular hydrocarbons (CHCs) are organic compounds of the surface lipid layer, which function as a barrier against water loss and xenobiotic penetration, while also serving as chemical signals. Plasticity of CHC profiles can vary depending upon numerous biological and environmental factors. Here, we investigated potential sources of variation in CHC profiles of Nilaparvata lugens, Laodelphax striatellus and Sogatella furcifera, which are considered to be the most important rice pests in Asia. CHC profiles were quantified by GC/MS, and factors associated with variations were explored by conducting principal component analysis (PCA). Transcriptomes were further compared under different environmental conditions. The results demonstrated that CHC profiles differ among three species and change with different developmental stages, sexes, temperature, humidity and host plants. Genes involved in cuticular lipid biosynthesis pathways are modulated, which might explain why CHC profile...
The Journal of experimental biology, 2002
Large amounts of hydrocarbons accumulate during vitellogenesis in the developing basal oocytes of... more Large amounts of hydrocarbons accumulate during vitellogenesis in the developing basal oocytes of the German cockroach Blattella germanica (L.), and all ovarian hydrocarbons are deposited into an egg case (ootheca) during oviposition. Hydrocarbons are not synthesized by the ovaries, but are delivered by hemolymph lipoproteins and accumulate within the basal oocytes. A native B. germanica hydrocarbon, [(3)H]3,11-dimethylnonacosane, injected into adult females of various ages, was taken up by the ovaries in relation to oocyte growth. Ovarian uptake of the hydrocarbon was low in day 0-1 females, increased dramatically between days 3 and 6 and declined sharply through oviposition on day 8-9; ovarian uptake of the hydrocarbon was low during a 21-day pregnancy that followed. [1-(14)C]Propionate, which becomes incorporated into methyl-branched hydrocarbons, was injected into 5-day-old vitellogenic females to monitor the de novo biosynthesis of hydrocarbons and the time course of hydrocarbo...
Journal of economic entomology, Jan 2, 2018
The green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), is an important pest of ma... more The green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), is an important pest of many crops in the world and a vector of more than 100 plant viruses. It is a major pest of Brassica vegetables such as Chinese cabbage in northern China. Chemical control is extensively used to manage this aphid around the world; however, development of insecticide resistance has been a major obstacle facing growers. Host plant resistance in Chinese cabbage against M. persicae has not been reported yet. In this study, we investigated the resistance categories in seven Chinese cabbage cultivars against M. persicae. The resistance categories of these cultivars included antixenosis, antibiosis, and tolerance related to leaf color and wax content. The cultivar 'Yuanbao' had antibiotic and tolerance effects on the aphid. The rate of intrinsic increase (rm) of M. persicae was lower on Yuanbao compared with the other six cultivars. Yuanbao also had the highest antibiosis against the aphid...
Nature communications, Mar 20, 2018
Many cockroach species have adapted to urban environments, and some have been serious pests of pu... more Many cockroach species have adapted to urban environments, and some have been serious pests of public health in the tropics and subtropics. Here, we present the 3.38-Gb genome and a consensus gene set of the American cockroach, Periplaneta americana. We report insights from both genomic and functional investigations into the underlying basis of its adaptation to urban environments and developmental plasticity. In comparison with other insects, expansions of gene families in P. americana exist for most core gene families likely associated with environmental adaptation, such as chemoreception and detoxification. Multiple pathways regulating metamorphic development are well conserved, and RNAi experiments inform on key roles of 20-hydroxyecdysone, juvenile hormone, insulin, and decapentaplegic signals in regulating plasticity. Our analyses reveal a high level of sequence identity in genes between the American cockroach and two termite species, advancing it as a valuable model to study ...
Journal of insect physiology, Nov 1, 2017
A rise in atmospheric carbon dioxide concentration ([CO2]) and a warming climate are two of the m... more A rise in atmospheric carbon dioxide concentration ([CO2]) and a warming climate are two of the most conspicuous characteristics of global climate change in this century. However, studies addressing the combined impact of rising [CO2] and temperature on herbivore insect physiology are still limited. In this study we investigated the combined effects of elevated [CO2] and temperature on major antioxidative enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidases (POD) and detoxification enzymes of glutathione-S-transferases (GST) and acetylcholinesterase (AChE) in three consecutive generations of Bemisia tabaci Middle East-Asia Minor 1 (MEAM1, commonly known as B biotype) adults. The results indicated that the antioxidant capabilities of B. tabaci differed significantly during different treatments across different generations. Elevated [CO2] markedly increased POD, GST and AChE activities in the first generation, and SOD, CAT and GST activities in the second generat...
Journal of Asia-Pacific Entomology
J Insect Physiol, 1997
The adrenergicagonistsoctopamine,tyramine and clonidineinhibitedthe normal pheromonotropic action... more The adrenergicagonistsoctopamine,tyramine and clonidineinhibitedthe normal pheromonotropic action due to PBAN (pheromone biosynthesisactivating neuropeptide) in incubations of intersegmentaltissues that are situated between the 8th and 9th abdominalsegments of the moth ovipositortip. This inhibitionwas reversedin the presence of the adrenergicantagonists phentolamine,yohimbine and chlorpromazine.Incubations of 8th segments alone, which do not produce pheromone, resulted in elevated levels of intracellularcAMP in the presence of octopamine.The physiologicalsignificanceof this phenomenonis unclear. However, clonidine (an a, selective agonist) did not duplicate octopamine stimulation of intracellular cAMP in 8th segment cultures. In intersegmentalmembrane cultures clonidine successfully duplicated the octopamineinhibitionof both pheromoneand intracellularcAMP production.The physiological significanceof octopaminergicreceptors mediatingthe inhibitory response of intersegments was investigated by experiments i v i When PBAN was injected into photophase females the normal pheromonotropicactivity due to the injected PBAN dropped after 2h. In the presence of clonidine, normal peak stimulator levels were never attained and a faster decline was observed. Clonidine also inhibited the pheromonotropicresponse of 24h-decapitated females to PBAN. Adrenergic antagonistssuccessfully reversed the inhibitory effect of clonidine in decapitated females, but did not reverse the effect of clonidine in photophase females. In addition, when clonidine was injected into female moths during the scotophase normal peak pheromone titers were reduced although no effect on calling behavior was observed.
Peptides, Nov 30, 2006
Hormones in the insulin superfamily initiate a wide range of physiological processes in higher eu... more Hormones in the insulin superfamily initiate a wide range of physiological processes in higher eukaryotic organisms. Although insulin-like peptides (ILPs) are likely present in all arthropods, they have been characterized for only a few insect species, including Locusta migratoria (Orthoptera [28]); three species of Lepidoptera [57]; and two species of Diptera, the fruit fly, Drosophila melanogaster [7] and the mosquito, Anopheles gambiae [26,41]. Most insect ILP genes encode a single prepropeptide with a signal peptide and contiguous B, C, and A peptides. The propeptide is processed into an active form by linkage of the A and B peptides by disulfide bridges followed by proteolytic cleavage of the C peptide, as with insulin and related peptides in vertebrates. An exception is the insulin growth factors (IGFs), which possess a short C peptide p e p t i d e s x x x (2 0 0 6) x x x-x x x a r t i c l e i n f o Article history:
Insect Biochemistry and Molecular Biology
Journal of Insect Physiology
The Journal of experimental biology, 2002
Large amounts of hydrocarbons accumulate during vitellogenesis in the developing basal oocytes of... more Large amounts of hydrocarbons accumulate during vitellogenesis in the developing basal oocytes of the German cockroach Blattella germanica (L.), and all ovarian hydrocarbons are deposited into an egg case (ootheca) during oviposition. Hydrocarbons are not synthesized by the ovaries, but are delivered by hemolymph lipoproteins and accumulate within the basal oocytes. A native B. germanica hydrocarbon, [(3)H]3,11-dimethylnonacosane, injected into adult females of various ages, was taken up by the ovaries in relation to oocyte growth. Ovarian uptake of the hydrocarbon was low in day 0-1 females, increased dramatically between days 3 and 6 and declined sharply through oviposition on day 8-9; ovarian uptake of the hydrocarbon was low during a 21-day pregnancy that followed. [1-(14)C]Propionate, which becomes incorporated into methyl-branched hydrocarbons, was injected into 5-day-old vitellogenic females to monitor the de novo biosynthesis of hydrocarbons and the time course of hydrocarbo...
Metabolites, 2021
Benzoxazinoids are important secondary metabolites in gramineae plants and have inhibitory and to... more Benzoxazinoids are important secondary metabolites in gramineae plants and have inhibitory and toxic effects against a wide range of herbivore pests. However, the relationship between benzoxazinoid level and plant resistance to aphids remains controversial. In this study, we investigated the relationship between benzoxazinoids composition and concentration in wheat leaves and the resistance to the grain aphid Sitobion avenae. Overall, six benzoxazinoids were detected and identified by mass spectrometry based metabolites profiling, including three lactams, two hydroxamic acids, and one methyl derivative. The constitutive levels of these benzoxazinoids were significantly different among the wheat varieties/lines. However, none of these benzoxazinoids exhibited considerable correlation with aphid resistance. S. avenae feeding elevated the level of 2-O-β-D-glucopyranosyloxy-4,7-dimethoxy-(2H)-1,4-benzoxazin-3(4H)-one (HDMBOA-Glc) and reduced the level of 2-O-β-D-glucopyranosyloxy-4-hydr...
Scientific reports, Jan 20, 2016
Low proportion of essential amino acids (EAAs) is one of the barriers for animals to use phloem a... more Low proportion of essential amino acids (EAAs) is one of the barriers for animals to use phloem as a diet. Endosymbionts with EAAs synthesis functions are considered crucial for ameliorating the lack of EAAs in insects' diets. In this study, we transferred the insects from a cabbage-reared Myzus persicae population onto 3 new plant species including eggplant, tobacco and spinach. The performance on these plants was evaluated and the dynamics of endosymbionts in relation to this host alternation were recorded. We found that the EAAs ratio in phloem was largely determined by the concentrations of non-essential amino acids and the higher proportion of EAAs seemed to favor the population establishment on new plant species and the growth of primary endosymbionts inside insects, which indicated that nitrogen quality was an important factor for aphids to infest and spread on new plant hosts.
International Journal of Molecular Sciences, 2021
Insect cuticular hydrocarbons (CHCs) are organic compounds of the surface lipid layer, which func... more Insect cuticular hydrocarbons (CHCs) are organic compounds of the surface lipid layer, which function as a barrier against water loss and xenobiotic penetration, while also serving as chemical signals. Plasticity of CHC profiles can vary depending upon numerous biological and environmental factors. Here, we investigated potential sources of variation in CHC profiles of Nilaparvata lugens, Laodelphax striatellus and Sogatella furcifera, which are considered to be the most important rice pests in Asia. CHC profiles were quantified by GC/MS, and factors associated with variations were explored by conducting principal component analysis (PCA). Transcriptomes were further compared under different environmental conditions. The results demonstrated that CHC profiles differ among three species and change with different developmental stages, sexes, temperature, humidity and host plants. Genes involved in cuticular lipid biosynthesis pathways are modulated, which might explain why CHC profile...
The Journal of experimental biology, 2002
Large amounts of hydrocarbons accumulate during vitellogenesis in the developing basal oocytes of... more Large amounts of hydrocarbons accumulate during vitellogenesis in the developing basal oocytes of the German cockroach Blattella germanica (L.), and all ovarian hydrocarbons are deposited into an egg case (ootheca) during oviposition. Hydrocarbons are not synthesized by the ovaries, but are delivered by hemolymph lipoproteins and accumulate within the basal oocytes. A native B. germanica hydrocarbon, [(3)H]3,11-dimethylnonacosane, injected into adult females of various ages, was taken up by the ovaries in relation to oocyte growth. Ovarian uptake of the hydrocarbon was low in day 0-1 females, increased dramatically between days 3 and 6 and declined sharply through oviposition on day 8-9; ovarian uptake of the hydrocarbon was low during a 21-day pregnancy that followed. [1-(14)C]Propionate, which becomes incorporated into methyl-branched hydrocarbons, was injected into 5-day-old vitellogenic females to monitor the de novo biosynthesis of hydrocarbons and the time course of hydrocarbo...
Journal of economic entomology, Jan 2, 2018
The green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), is an important pest of ma... more The green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae), is an important pest of many crops in the world and a vector of more than 100 plant viruses. It is a major pest of Brassica vegetables such as Chinese cabbage in northern China. Chemical control is extensively used to manage this aphid around the world; however, development of insecticide resistance has been a major obstacle facing growers. Host plant resistance in Chinese cabbage against M. persicae has not been reported yet. In this study, we investigated the resistance categories in seven Chinese cabbage cultivars against M. persicae. The resistance categories of these cultivars included antixenosis, antibiosis, and tolerance related to leaf color and wax content. The cultivar 'Yuanbao' had antibiotic and tolerance effects on the aphid. The rate of intrinsic increase (rm) of M. persicae was lower on Yuanbao compared with the other six cultivars. Yuanbao also had the highest antibiosis against the aphid...
Nature communications, Mar 20, 2018
Many cockroach species have adapted to urban environments, and some have been serious pests of pu... more Many cockroach species have adapted to urban environments, and some have been serious pests of public health in the tropics and subtropics. Here, we present the 3.38-Gb genome and a consensus gene set of the American cockroach, Periplaneta americana. We report insights from both genomic and functional investigations into the underlying basis of its adaptation to urban environments and developmental plasticity. In comparison with other insects, expansions of gene families in P. americana exist for most core gene families likely associated with environmental adaptation, such as chemoreception and detoxification. Multiple pathways regulating metamorphic development are well conserved, and RNAi experiments inform on key roles of 20-hydroxyecdysone, juvenile hormone, insulin, and decapentaplegic signals in regulating plasticity. Our analyses reveal a high level of sequence identity in genes between the American cockroach and two termite species, advancing it as a valuable model to study ...
Journal of insect physiology, Nov 1, 2017
A rise in atmospheric carbon dioxide concentration ([CO2]) and a warming climate are two of the m... more A rise in atmospheric carbon dioxide concentration ([CO2]) and a warming climate are two of the most conspicuous characteristics of global climate change in this century. However, studies addressing the combined impact of rising [CO2] and temperature on herbivore insect physiology are still limited. In this study we investigated the combined effects of elevated [CO2] and temperature on major antioxidative enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidases (POD) and detoxification enzymes of glutathione-S-transferases (GST) and acetylcholinesterase (AChE) in three consecutive generations of Bemisia tabaci Middle East-Asia Minor 1 (MEAM1, commonly known as B biotype) adults. The results indicated that the antioxidant capabilities of B. tabaci differed significantly during different treatments across different generations. Elevated [CO2] markedly increased POD, GST and AChE activities in the first generation, and SOD, CAT and GST activities in the second generat...
Journal of Asia-Pacific Entomology
J Insect Physiol, 1997
The adrenergicagonistsoctopamine,tyramine and clonidineinhibitedthe normal pheromonotropic action... more The adrenergicagonistsoctopamine,tyramine and clonidineinhibitedthe normal pheromonotropic action due to PBAN (pheromone biosynthesisactivating neuropeptide) in incubations of intersegmentaltissues that are situated between the 8th and 9th abdominalsegments of the moth ovipositortip. This inhibitionwas reversedin the presence of the adrenergicantagonists phentolamine,yohimbine and chlorpromazine.Incubations of 8th segments alone, which do not produce pheromone, resulted in elevated levels of intracellularcAMP in the presence of octopamine.The physiologicalsignificanceof this phenomenonis unclear. However, clonidine (an a, selective agonist) did not duplicate octopamine stimulation of intracellular cAMP in 8th segment cultures. In intersegmentalmembrane cultures clonidine successfully duplicated the octopamineinhibitionof both pheromoneand intracellularcAMP production.The physiological significanceof octopaminergicreceptors mediatingthe inhibitory response of intersegments was investigated by experiments i v i When PBAN was injected into photophase females the normal pheromonotropicactivity due to the injected PBAN dropped after 2h. In the presence of clonidine, normal peak stimulator levels were never attained and a faster decline was observed. Clonidine also inhibited the pheromonotropicresponse of 24h-decapitated females to PBAN. Adrenergic antagonistssuccessfully reversed the inhibitory effect of clonidine in decapitated females, but did not reverse the effect of clonidine in photophase females. In addition, when clonidine was injected into female moths during the scotophase normal peak pheromone titers were reduced although no effect on calling behavior was observed.
Peptides, Nov 30, 2006
Hormones in the insulin superfamily initiate a wide range of physiological processes in higher eu... more Hormones in the insulin superfamily initiate a wide range of physiological processes in higher eukaryotic organisms. Although insulin-like peptides (ILPs) are likely present in all arthropods, they have been characterized for only a few insect species, including Locusta migratoria (Orthoptera [28]); three species of Lepidoptera [57]; and two species of Diptera, the fruit fly, Drosophila melanogaster [7] and the mosquito, Anopheles gambiae [26,41]. Most insect ILP genes encode a single prepropeptide with a signal peptide and contiguous B, C, and A peptides. The propeptide is processed into an active form by linkage of the A and B peptides by disulfide bridges followed by proteolytic cleavage of the C peptide, as with insulin and related peptides in vertebrates. An exception is the insulin growth factors (IGFs), which possess a short C peptide p e p t i d e s x x x (2 0 0 6) x x x-x x x a r t i c l e i n f o Article history:
Insect Biochemistry and Molecular Biology
Journal of Insect Physiology
The Journal of experimental biology, 2002
Large amounts of hydrocarbons accumulate during vitellogenesis in the developing basal oocytes of... more Large amounts of hydrocarbons accumulate during vitellogenesis in the developing basal oocytes of the German cockroach Blattella germanica (L.), and all ovarian hydrocarbons are deposited into an egg case (ootheca) during oviposition. Hydrocarbons are not synthesized by the ovaries, but are delivered by hemolymph lipoproteins and accumulate within the basal oocytes. A native B. germanica hydrocarbon, [(3)H]3,11-dimethylnonacosane, injected into adult females of various ages, was taken up by the ovaries in relation to oocyte growth. Ovarian uptake of the hydrocarbon was low in day 0-1 females, increased dramatically between days 3 and 6 and declined sharply through oviposition on day 8-9; ovarian uptake of the hydrocarbon was low during a 21-day pregnancy that followed. [1-(14)C]Propionate, which becomes incorporated into methyl-branched hydrocarbons, was injected into 5-day-old vitellogenic females to monitor the de novo biosynthesis of hydrocarbons and the time course of hydrocarbo...