Jelena Janjic | Duquesne University (original) (raw)

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Papers by Jelena Janjic

Research paper thumbnail of Inflammation Driven by Overexpression of the Hypoglycosylated Abnormal Mucin 1 (MUC1) Links Inflammatory Bowel Disease and Pancreatitis

Research paper thumbnail of Biphenyl C-cyclopropylalkylamides: New scaffolds for targeting estrogen receptor

Bioorganic & Medicinal Chemistry Letters, 2009

Research paper thumbnail of Self-delivering nanoemulsions for dual fluorine-19 MRI and fluorescence detection

Journal of The American Chemical Society, 2008

We report the design, synthesis, and biological testing of highly stable, nontoxic perfluoropolye... more We report the design, synthesis, and biological testing of highly stable, nontoxic perfluoropolyether (PFPE) nanoemulsions for dual 19F MRI-fluorescence detection. A linear PFPE polymer was covalently conjugated to common fluorescent dyes (FITC, Alexa647 and BODIPy-TR), mixed with pluronic F68 and linear polyethyleneimine (PEI), and emulsified by microfluidization. Prepared nanoemulsions (<200 nm) were readily taken up by both phagocytic and non-phagocytic cells in vitro after a short (approximately 3 h) co-incubation. Following cell administration in vivo, 19F MRI selectively visualizes cell migration. Exemplary in vivo MRI images are presented of T cells labeled with a dual-mode nanoemulsion in a BALB/c mouse. Fluorescence detection enables fluorescent microscopy and FACS analysis of labeled cells, as demonstrated in several immune cell types including Jurkat cells, primary T cells and dendritic cells. The intracellular fluorescence signal is directly proportional to the 19F NMR signal and can be used to calibrate cell loading in vitro.

Research paper thumbnail of In vivo cytometry of antigen-specific t cells using 19F MRI

Magnetic Resonance in Medicine, 2009

Research paper thumbnail of New antiestrogens from a library screen of homoallylic amides, allylic amides, and C-cyclopropylalkylamides

Bioorganic & Medicinal Chemistry, 2005

A new structural scaffold for antiestrogens was identified from the cell-based screening of trans... more A new structural scaffold for antiestrogens was identified from the cell-based screening of transcriptional regulation properties of a 67-member library of homoallylic amides, allylic amides, and C-cyclopropylalkylamides. C-Cyclopropylalkylamide 3a (O-ethyl-N-{2-[(1S*,2R*)-2-{(R*)-[(diphenylphosphinoyl)amino](phenyl)methyl}cyclopropyl]ethyl}-N-[(4-methylphenyl)sulfonyl]carbamate) had antagonistic activity similar to that of tamoxifen and was further evaluated. Compound 3a inhibited estradiol-induced proliferation of the ER-positive MCF-7 cells but had no effect on ER-negative MDA-MB231 human breast cancer cells. Furthermore, high micromolar concentrations of 3a exhibited minimal cytotoxicity to the ER-negative line. The biological activities of the enantiomers of 3a did not differ from one another nor from that of racemic 3a.

Research paper thumbnail of Microwave-Assisted “Libraries from Libraries” Approach toward the Synthesis of Allyl and C -Cyclopropylalkylamides

Journal of Combinatorial Chemistry, 2005

Research paper thumbnail of Functional assessment of human dendritic cells labeled for in vivo (19)F magnetic resonance imaging cell tracking

Research paper thumbnail of In vivo cytometry of antigen-specific t cells using 19 F MRI

Magnetic Resonance in Medicine, 2009

Research paper thumbnail of Fluorine-containing nanoemulsions for MRI cell tracking

Wiley Interdisciplinary Reviews-nanomedicine and Nanobiotechnology, 2009

In this article we review the chemistry and nanoemulsion formulation of perfluorocarbons used for... more In this article we review the chemistry and nanoemulsion formulation of perfluorocarbons used for in vivo19F MRI cell tracking. In this application, cells of interest are labeled in culture using a perfluorocarbon nanoemulsion. Labeled cells are introduced into a subject and tracked using 19F MRI or NMR spectroscopy. In the same imaging session, a high-resolution, conventional (1H) image can be used to place the 19F-labeled cells into anatomical context. Perfluorocarbon-based 19F cell tracking is a useful technology because of the high specificity for labeled cells, ability to quantify cell accumulations, and biocompatibility. This technology can be widely applied to studies of inflammation, cellular regenerative medicine, and immunotherapy. Copyright © 2009 John Wiley & Sons, Inc.For further resources related to this article, please visit the WIREs website.

Research paper thumbnail of Microwave-Assisted Synthesis of Allylic Amines: Considerable Rate Acceleration in the Hydrozirconation—Transmetalation—Aldimine Addition Sequence

Cheminform, 2004

For Abstract see ChemInform Abstract in Full Text.

Research paper thumbnail of Microwave-assisted synthesis of allylic amines: considerable rate acceleration in the hydrozirconation?transmetalation?aldimine addition sequenceElectronic supplementary information (ESI) available: experimental procedures and 1H and 13C NMR spectra for all new compounds. See http://www.rsc.org/s...

Organic & Biomolecular Chemistry, 2004

Research paper thumbnail of Inflammation Driven by Overexpression of the Hypoglycosylated Abnormal Mucin 1 (MUC1) Links Inflammatory Bowel Disease and Pancreatitis

Research paper thumbnail of Biphenyl C-cyclopropylalkylamides: New scaffolds for targeting estrogen receptor

Bioorganic & Medicinal Chemistry Letters, 2009

Research paper thumbnail of Self-delivering nanoemulsions for dual fluorine-19 MRI and fluorescence detection

Journal of The American Chemical Society, 2008

We report the design, synthesis, and biological testing of highly stable, nontoxic perfluoropolye... more We report the design, synthesis, and biological testing of highly stable, nontoxic perfluoropolyether (PFPE) nanoemulsions for dual 19F MRI-fluorescence detection. A linear PFPE polymer was covalently conjugated to common fluorescent dyes (FITC, Alexa647 and BODIPy-TR), mixed with pluronic F68 and linear polyethyleneimine (PEI), and emulsified by microfluidization. Prepared nanoemulsions (<200 nm) were readily taken up by both phagocytic and non-phagocytic cells in vitro after a short (approximately 3 h) co-incubation. Following cell administration in vivo, 19F MRI selectively visualizes cell migration. Exemplary in vivo MRI images are presented of T cells labeled with a dual-mode nanoemulsion in a BALB/c mouse. Fluorescence detection enables fluorescent microscopy and FACS analysis of labeled cells, as demonstrated in several immune cell types including Jurkat cells, primary T cells and dendritic cells. The intracellular fluorescence signal is directly proportional to the 19F NMR signal and can be used to calibrate cell loading in vitro.

Research paper thumbnail of In vivo cytometry of antigen-specific t cells using 19F MRI

Magnetic Resonance in Medicine, 2009

Research paper thumbnail of New antiestrogens from a library screen of homoallylic amides, allylic amides, and C-cyclopropylalkylamides

Bioorganic & Medicinal Chemistry, 2005

A new structural scaffold for antiestrogens was identified from the cell-based screening of trans... more A new structural scaffold for antiestrogens was identified from the cell-based screening of transcriptional regulation properties of a 67-member library of homoallylic amides, allylic amides, and C-cyclopropylalkylamides. C-Cyclopropylalkylamide 3a (O-ethyl-N-{2-[(1S*,2R*)-2-{(R*)-[(diphenylphosphinoyl)amino](phenyl)methyl}cyclopropyl]ethyl}-N-[(4-methylphenyl)sulfonyl]carbamate) had antagonistic activity similar to that of tamoxifen and was further evaluated. Compound 3a inhibited estradiol-induced proliferation of the ER-positive MCF-7 cells but had no effect on ER-negative MDA-MB231 human breast cancer cells. Furthermore, high micromolar concentrations of 3a exhibited minimal cytotoxicity to the ER-negative line. The biological activities of the enantiomers of 3a did not differ from one another nor from that of racemic 3a.

Research paper thumbnail of Microwave-Assisted “Libraries from Libraries” Approach toward the Synthesis of Allyl and C -Cyclopropylalkylamides

Journal of Combinatorial Chemistry, 2005

Research paper thumbnail of Functional assessment of human dendritic cells labeled for in vivo (19)F magnetic resonance imaging cell tracking

Research paper thumbnail of In vivo cytometry of antigen-specific t cells using 19 F MRI

Magnetic Resonance in Medicine, 2009

Research paper thumbnail of Fluorine-containing nanoemulsions for MRI cell tracking

Wiley Interdisciplinary Reviews-nanomedicine and Nanobiotechnology, 2009

In this article we review the chemistry and nanoemulsion formulation of perfluorocarbons used for... more In this article we review the chemistry and nanoemulsion formulation of perfluorocarbons used for in vivo19F MRI cell tracking. In this application, cells of interest are labeled in culture using a perfluorocarbon nanoemulsion. Labeled cells are introduced into a subject and tracked using 19F MRI or NMR spectroscopy. In the same imaging session, a high-resolution, conventional (1H) image can be used to place the 19F-labeled cells into anatomical context. Perfluorocarbon-based 19F cell tracking is a useful technology because of the high specificity for labeled cells, ability to quantify cell accumulations, and biocompatibility. This technology can be widely applied to studies of inflammation, cellular regenerative medicine, and immunotherapy. Copyright © 2009 John Wiley & Sons, Inc.For further resources related to this article, please visit the WIREs website.

Research paper thumbnail of Microwave-Assisted Synthesis of Allylic Amines: Considerable Rate Acceleration in the Hydrozirconation—Transmetalation—Aldimine Addition Sequence

Cheminform, 2004

For Abstract see ChemInform Abstract in Full Text.

Research paper thumbnail of Microwave-assisted synthesis of allylic amines: considerable rate acceleration in the hydrozirconation?transmetalation?aldimine addition sequenceElectronic supplementary information (ESI) available: experimental procedures and 1H and 13C NMR spectra for all new compounds. See http://www.rsc.org/s...

Organic & Biomolecular Chemistry, 2004

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